Encapsulation state of messenger RNA inside lipid nanoparticles

Understanding the structure of messenger RNA (mRNA) lipid nanoparticles, and specifically the microenvironment of the mRNA molecules within these entities, is fundamental to advancing their biomedical potential. Here, we show that a permeating cationic dye, thionine, can serve as a cryogenic electro...

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Detalles Bibliográficos
Autores principales: Brader, Mark L., Williams, Sean J., Banks, Jessica M., Hui, Wong H., Zhou, Z. Hong, Jin, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2021
Materias:
Biophysical Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390897/
https://www.ncbi.nlm.nih.gov/pubmed/33773963
http://dx.doi.org/10.1016/j.bpj.2021.03.012
Descripción
Sumario:Understanding the structure of messenger RNA (mRNA) lipid nanoparticles, and specifically the microenvironment of the mRNA molecules within these entities, is fundamental to advancing their biomedical potential. Here, we show that a permeating cationic dye, thionine, can serve as a cryogenic electron microscopy contrasting agent by binding selectively to encapsulated mRNA without disturbing lipid nanoparticle morphology. Cryo-electron microscopy images identify the mRNA location, revealing that mRNA may exist within solvent-filled cavities or may be substantially lipid associated.

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