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Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka

Emerging renal biomarkers (e.g., kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)) are thought to be highly sensitive in diagnosing renal injury. However, global data on reference intervals for emerging biomarkers in younger populations are lacking. Here, we aim...

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Autores principales: De Silva, P. Mangala C. S., Gunasekara, T. D. K. S. C., Gunarathna, S. D., Sandamini, P. M. M. A., Pinipa, R. A. I., Ekanayake, E. M. D. V., Thakshila, W. A. K. G., Jayasinghe, S. S., Chandana, E. P. S., Jayasundara, Nishad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391325/
https://www.ncbi.nlm.nih.gov/pubmed/34438575
http://dx.doi.org/10.3390/children8080684
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author De Silva, P. Mangala C. S.
Gunasekara, T. D. K. S. C.
Gunarathna, S. D.
Sandamini, P. M. M. A.
Pinipa, R. A. I.
Ekanayake, E. M. D. V.
Thakshila, W. A. K. G.
Jayasinghe, S. S.
Chandana, E. P. S.
Jayasundara, Nishad
author_facet De Silva, P. Mangala C. S.
Gunasekara, T. D. K. S. C.
Gunarathna, S. D.
Sandamini, P. M. M. A.
Pinipa, R. A. I.
Ekanayake, E. M. D. V.
Thakshila, W. A. K. G.
Jayasinghe, S. S.
Chandana, E. P. S.
Jayasundara, Nishad
author_sort De Silva, P. Mangala C. S.
collection PubMed
description Emerging renal biomarkers (e.g., kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)) are thought to be highly sensitive in diagnosing renal injury. However, global data on reference intervals for emerging biomarkers in younger populations are lacking. Here, we aimed to determine reference intervals for KIM-1 and NGAL across a pediatric population in Sri Lanka; a country significantly impacted by the emergence of chronic kidney disease of unexplained etiology (CKDu). Urine samples were collected from children (10–18 years) with no prior record of renal diseases from the dry climatic zone of Sri Lanka (N = 909). Urinary KIM-1 and NGAL concentrations were determined using the enzyme-linked immunosorbent assay (ELISA) and adjusted to urinary creatinine. Biomarker levels were stratified by age and gender, and reference intervals derived with quantile regression (2.5th, 50th, and 97.5th quantiles) were expressed at 95% CI. The range of median reference intervals for urinary KIM-1 and NGAL in children were 0.081–0.426 ng/mg Cr, 2.966–4.850 ng/mg Cr for males, and 0.0780–0.5076 ng/mg Cr, 2.0850–3.4960 ng/mg Cr for females, respectively. Renal biomarkers showed weak correlations with age, gender, ACR, and BMI. Our findings provide reference intervals to facilitate screening to detect early renal damage, especially in rural communities that are impacted by CKDu.
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spelling pubmed-83913252021-08-28 Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka De Silva, P. Mangala C. S. Gunasekara, T. D. K. S. C. Gunarathna, S. D. Sandamini, P. M. M. A. Pinipa, R. A. I. Ekanayake, E. M. D. V. Thakshila, W. A. K. G. Jayasinghe, S. S. Chandana, E. P. S. Jayasundara, Nishad Children (Basel) Article Emerging renal biomarkers (e.g., kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)) are thought to be highly sensitive in diagnosing renal injury. However, global data on reference intervals for emerging biomarkers in younger populations are lacking. Here, we aimed to determine reference intervals for KIM-1 and NGAL across a pediatric population in Sri Lanka; a country significantly impacted by the emergence of chronic kidney disease of unexplained etiology (CKDu). Urine samples were collected from children (10–18 years) with no prior record of renal diseases from the dry climatic zone of Sri Lanka (N = 909). Urinary KIM-1 and NGAL concentrations were determined using the enzyme-linked immunosorbent assay (ELISA) and adjusted to urinary creatinine. Biomarker levels were stratified by age and gender, and reference intervals derived with quantile regression (2.5th, 50th, and 97.5th quantiles) were expressed at 95% CI. The range of median reference intervals for urinary KIM-1 and NGAL in children were 0.081–0.426 ng/mg Cr, 2.966–4.850 ng/mg Cr for males, and 0.0780–0.5076 ng/mg Cr, 2.0850–3.4960 ng/mg Cr for females, respectively. Renal biomarkers showed weak correlations with age, gender, ACR, and BMI. Our findings provide reference intervals to facilitate screening to detect early renal damage, especially in rural communities that are impacted by CKDu. MDPI 2021-08-09 /pmc/articles/PMC8391325/ /pubmed/34438575 http://dx.doi.org/10.3390/children8080684 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Silva, P. Mangala C. S.
Gunasekara, T. D. K. S. C.
Gunarathna, S. D.
Sandamini, P. M. M. A.
Pinipa, R. A. I.
Ekanayake, E. M. D. V.
Thakshila, W. A. K. G.
Jayasinghe, S. S.
Chandana, E. P. S.
Jayasundara, Nishad
Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka
title Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka
title_full Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka
title_fullStr Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka
title_full_unstemmed Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka
title_short Urinary Biomarkers of Renal Injury KIM-1 and NGAL: Reference Intervals for Healthy Pediatric Population in Sri Lanka
title_sort urinary biomarkers of renal injury kim-1 and ngal: reference intervals for healthy pediatric population in sri lanka
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391325/
https://www.ncbi.nlm.nih.gov/pubmed/34438575
http://dx.doi.org/10.3390/children8080684
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