A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization

Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dyn...

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Autores principales: Nimse, Satish Balasaheb, Song, Keum-Soo, Warkad, Shrikant Dashrath, Kim, Taisun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391390/
https://www.ncbi.nlm.nih.gov/pubmed/34436067
http://dx.doi.org/10.3390/bios11080265
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author Nimse, Satish Balasaheb
Song, Keum-Soo
Warkad, Shrikant Dashrath
Kim, Taisun
author_facet Nimse, Satish Balasaheb
Song, Keum-Soo
Warkad, Shrikant Dashrath
Kim, Taisun
author_sort Nimse, Satish Balasaheb
collection PubMed
description Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dynamic range of 10(1) to 10(4) copies per test. The newly developed SWAT method allows achieving highly sensitive and highly specific DNA hybridizations. The detection and discrimination of the MTB and NTM strain in the clinical samples with the SBR and SNP discrimination ratios higher than 160:1 indicate the high clinical applicability of the SWAT.
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spelling pubmed-83913902021-08-28 A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization Nimse, Satish Balasaheb Song, Keum-Soo Warkad, Shrikant Dashrath Kim, Taisun Biosensors (Basel) Article Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dynamic range of 10(1) to 10(4) copies per test. The newly developed SWAT method allows achieving highly sensitive and highly specific DNA hybridizations. The detection and discrimination of the MTB and NTM strain in the clinical samples with the SBR and SNP discrimination ratios higher than 160:1 indicate the high clinical applicability of the SWAT. MDPI 2021-08-06 /pmc/articles/PMC8391390/ /pubmed/34436067 http://dx.doi.org/10.3390/bios11080265 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nimse, Satish Balasaheb
Song, Keum-Soo
Warkad, Shrikant Dashrath
Kim, Taisun
A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
title A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
title_full A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
title_fullStr A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
title_full_unstemmed A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
title_short A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
title_sort novel method that allows snp discrimination with 160:1 ratio for biosensors based on dna-dna hybridization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391390/
https://www.ncbi.nlm.nih.gov/pubmed/34436067
http://dx.doi.org/10.3390/bios11080265
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