Cargando…
Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy
Photodynamic therapy (PDT) is a clinically approved, minimally invasive treatment for malignant tumors. Protoporphyrin IX (PpIX), derived from 5-aminolevulinic acid (5-ALA) as the prodrug, is one of the photosensitizers used in PDT. Recently, we reported a significant difference in response to 5-ALA...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391456/ https://www.ncbi.nlm.nih.gov/pubmed/34439326 http://dx.doi.org/10.3390/cancers13164174 |
_version_ | 1783743278894546944 |
---|---|
author | Osaki, Tomohiro Kunisue, Narumi Ota, Urara Imazato, Hideo Ishii, Takuya Takahashi, Kiwamu Ishizuka, Masahiro Tanaka, Tohru Okamoto, Yoshiharu |
author_facet | Osaki, Tomohiro Kunisue, Narumi Ota, Urara Imazato, Hideo Ishii, Takuya Takahashi, Kiwamu Ishizuka, Masahiro Tanaka, Tohru Okamoto, Yoshiharu |
author_sort | Osaki, Tomohiro |
collection | PubMed |
description | Photodynamic therapy (PDT) is a clinically approved, minimally invasive treatment for malignant tumors. Protoporphyrin IX (PpIX), derived from 5-aminolevulinic acid (5-ALA) as the prodrug, is one of the photosensitizers used in PDT. Recently, we reported a significant difference in response to 5-ALA-mediated PDT treatment in two canine primary lung adenocarcinoma cell lines (sensitive to PDT: HDC cells, resistant to PDT: LuBi cells). This study aimed to examine the difference in cytotoxicity of 5-ALA-mediated PDT in these cells. Although intracellular PpIX levels before irradiation were similar between HDC and LuBi cells, the percentage of ROS-positive cells and apoptotic cells in LuBi cells treated with 5-ALA-mediated PDT was significantly lower than that in HDC cells treated with 5-ALA-mediated PDT. A high dosage of the NO donor, DETA NONOate, significantly increased the cytotoxicity of 5-ALA-mediated PDT against LuBi cells. These results suggest that the sensitivity of 5-ALA-mediated PDT might be correlated with NO. |
format | Online Article Text |
id | pubmed-8391456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83914562021-08-28 Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy Osaki, Tomohiro Kunisue, Narumi Ota, Urara Imazato, Hideo Ishii, Takuya Takahashi, Kiwamu Ishizuka, Masahiro Tanaka, Tohru Okamoto, Yoshiharu Cancers (Basel) Article Photodynamic therapy (PDT) is a clinically approved, minimally invasive treatment for malignant tumors. Protoporphyrin IX (PpIX), derived from 5-aminolevulinic acid (5-ALA) as the prodrug, is one of the photosensitizers used in PDT. Recently, we reported a significant difference in response to 5-ALA-mediated PDT treatment in two canine primary lung adenocarcinoma cell lines (sensitive to PDT: HDC cells, resistant to PDT: LuBi cells). This study aimed to examine the difference in cytotoxicity of 5-ALA-mediated PDT in these cells. Although intracellular PpIX levels before irradiation were similar between HDC and LuBi cells, the percentage of ROS-positive cells and apoptotic cells in LuBi cells treated with 5-ALA-mediated PDT was significantly lower than that in HDC cells treated with 5-ALA-mediated PDT. A high dosage of the NO donor, DETA NONOate, significantly increased the cytotoxicity of 5-ALA-mediated PDT against LuBi cells. These results suggest that the sensitivity of 5-ALA-mediated PDT might be correlated with NO. MDPI 2021-08-19 /pmc/articles/PMC8391456/ /pubmed/34439326 http://dx.doi.org/10.3390/cancers13164174 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Osaki, Tomohiro Kunisue, Narumi Ota, Urara Imazato, Hideo Ishii, Takuya Takahashi, Kiwamu Ishizuka, Masahiro Tanaka, Tohru Okamoto, Yoshiharu Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy |
title | Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy |
title_full | Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy |
title_fullStr | Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy |
title_full_unstemmed | Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy |
title_short | Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy |
title_sort | mechanism of differential susceptibility of two (canine lung adenocarcinoma) cell lines to 5-aminolevulinic acid-mediated photodynamic therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391456/ https://www.ncbi.nlm.nih.gov/pubmed/34439326 http://dx.doi.org/10.3390/cancers13164174 |
work_keys_str_mv | AT osakitomohiro mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT kunisuenarumi mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT otaurara mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT imazatohideo mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT ishiitakuya mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT takahashikiwamu mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT ishizukamasahiro mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT tanakatohru mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy AT okamotoyoshiharu mechanismofdifferentialsusceptibilityoftwocaninelungadenocarcinomacelllinesto5aminolevulinicacidmediatedphotodynamictherapy |