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Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection
Massive platelet activation and thrombotic events characterize severe COVID-19, highlighting their critical role in SARS-CoV-2-induced immunopathology. Since there is a well-described expansion of myeloid-derived suppressor cells (MDSC) in severe COVID-19, we evaluated their possible role in platele...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391818/ https://www.ncbi.nlm.nih.gov/pubmed/34440879 http://dx.doi.org/10.3390/cells10082111 |
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author | Sacchi, Alessandra Grassi, Germana Notari, Stefania Gili, Simona Bordoni, Veronica Tartaglia, Eleonora Casetti, Rita Cimini, Eleonora Mariotti, Davide Garotto, Gabriele Beccacece, Alessia Marchioni, Luisa Bibas, Michele Nicastri, Emanuele Ippolito, Giuseppe Agrati, Chiara |
author_facet | Sacchi, Alessandra Grassi, Germana Notari, Stefania Gili, Simona Bordoni, Veronica Tartaglia, Eleonora Casetti, Rita Cimini, Eleonora Mariotti, Davide Garotto, Gabriele Beccacece, Alessia Marchioni, Luisa Bibas, Michele Nicastri, Emanuele Ippolito, Giuseppe Agrati, Chiara |
author_sort | Sacchi, Alessandra |
collection | PubMed |
description | Massive platelet activation and thrombotic events characterize severe COVID-19, highlighting their critical role in SARS-CoV-2-induced immunopathology. Since there is a well-described expansion of myeloid-derived suppressor cells (MDSC) in severe COVID-19, we evaluated their possible role in platelet activation during SARS-CoV-2 infection. During COVID-19, a lower plasmatic L-arginine level was observed compared to healthy donors, which correlated with MDSC frequency. Additionally, activated GPIIb/IIIa complex (PAC-1) expression was higher on platelets from severe COVID-19 patients compared to healthy controls and inversely correlated with L-arginine plasmatic concentration. Notably, MDSC were able to induce PAC-1 expression in vitro by reducing L-arginine concentration, indicating a direct role of PMN-MDSC in platelet activation. Accordingly, we found a positive correlation between ex vivo platelet PAC-1 expression and PMN-MDSC frequency. Overall, our data demonstrate the involvement of PMN-MDSC in triggering platelet activation during COVID-19, highlighting a novel role of MDSC in driving COVID-19 pathogenesis. |
format | Online Article Text |
id | pubmed-8391818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83918182021-08-28 Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection Sacchi, Alessandra Grassi, Germana Notari, Stefania Gili, Simona Bordoni, Veronica Tartaglia, Eleonora Casetti, Rita Cimini, Eleonora Mariotti, Davide Garotto, Gabriele Beccacece, Alessia Marchioni, Luisa Bibas, Michele Nicastri, Emanuele Ippolito, Giuseppe Agrati, Chiara Cells Brief Report Massive platelet activation and thrombotic events characterize severe COVID-19, highlighting their critical role in SARS-CoV-2-induced immunopathology. Since there is a well-described expansion of myeloid-derived suppressor cells (MDSC) in severe COVID-19, we evaluated their possible role in platelet activation during SARS-CoV-2 infection. During COVID-19, a lower plasmatic L-arginine level was observed compared to healthy donors, which correlated with MDSC frequency. Additionally, activated GPIIb/IIIa complex (PAC-1) expression was higher on platelets from severe COVID-19 patients compared to healthy controls and inversely correlated with L-arginine plasmatic concentration. Notably, MDSC were able to induce PAC-1 expression in vitro by reducing L-arginine concentration, indicating a direct role of PMN-MDSC in platelet activation. Accordingly, we found a positive correlation between ex vivo platelet PAC-1 expression and PMN-MDSC frequency. Overall, our data demonstrate the involvement of PMN-MDSC in triggering platelet activation during COVID-19, highlighting a novel role of MDSC in driving COVID-19 pathogenesis. MDPI 2021-08-17 /pmc/articles/PMC8391818/ /pubmed/34440879 http://dx.doi.org/10.3390/cells10082111 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Sacchi, Alessandra Grassi, Germana Notari, Stefania Gili, Simona Bordoni, Veronica Tartaglia, Eleonora Casetti, Rita Cimini, Eleonora Mariotti, Davide Garotto, Gabriele Beccacece, Alessia Marchioni, Luisa Bibas, Michele Nicastri, Emanuele Ippolito, Giuseppe Agrati, Chiara Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
title | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
title_full | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
title_fullStr | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
title_full_unstemmed | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
title_short | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
title_sort | expansion of myeloid derived suppressor cells contributes to platelet activation by l-arginine deprivation during sars-cov-2 infection |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391818/ https://www.ncbi.nlm.nih.gov/pubmed/34440879 http://dx.doi.org/10.3390/cells10082111 |
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