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Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended
SIMPLE SUMMARY: In population-based screens, tissue biopsy remains the standard practice for women with imaging that suggests breast cancer. We examined circulating microRNAs as minimally invasive diagnostic biomarkers to discriminate malignant from benign breast lesions. A retrospective cohort of p...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391908/ https://www.ncbi.nlm.nih.gov/pubmed/34439180 http://dx.doi.org/10.3390/cancers13164028 |
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author | Giussani, Marta Ciniselli, Chiara Maura De Cecco, Loris Lecchi, Mara Dugo, Matteo Gargiuli, Chiara Mariancini, Andrea Mancinelli, Elisa Cosentino, Giulia Veneroni, Silvia Paolini, Biagio Orlandi, Rosaria Gennaro, Massimiliano Iorio, Marilena Valeria Depretto, Catherine Ferranti, Claudio Sozzi, Gabriella Sensi, Marialuisa Colombo, Mario Paolo Scaperrotta, Gianfranco Tagliabue, Elda Verderio, Paolo |
author_facet | Giussani, Marta Ciniselli, Chiara Maura De Cecco, Loris Lecchi, Mara Dugo, Matteo Gargiuli, Chiara Mariancini, Andrea Mancinelli, Elisa Cosentino, Giulia Veneroni, Silvia Paolini, Biagio Orlandi, Rosaria Gennaro, Massimiliano Iorio, Marilena Valeria Depretto, Catherine Ferranti, Claudio Sozzi, Gabriella Sensi, Marialuisa Colombo, Mario Paolo Scaperrotta, Gianfranco Tagliabue, Elda Verderio, Paolo |
author_sort | Giussani, Marta |
collection | PubMed |
description | SIMPLE SUMMARY: In population-based screens, tissue biopsy remains the standard practice for women with imaging that suggests breast cancer. We examined circulating microRNAs as minimally invasive diagnostic biomarkers to discriminate malignant from benign breast lesions. A retrospective cohort of plasma samples divided into training and testing sets and a prospective cohort of women with suspicious imaging findings who underwent tissue biopsy were investigated through a global microRNA profile by OpenArray. Seven signatures, involving 5 specific miRNAs (miR-625, miR-423-5p, miR-370-3p, miR-181c, and miR-301b), were identified and validated in the testing set. Among the 7 signatures, the discriminatory performances of 5 of them were confirmed in the prospective cohort. ABSTRACT: In population-based screens, tissue biopsy remains the standard practice for women with imaging that suggests breast cancer. We examined circulating microRNAs as minimally invasive diagnostic biomarkers to discriminate malignant from benign breast lesions. miRNAs were analyzed by OpenArray in a retrospective cohort of plasma samples including 100 patients with malignant (T), 89 benign disease (B), and 99 healthy donors (HD) divided into training and testing sets and a prospective cohort (BABE) of 289 women with suspicious imaging findings who underwent tissue biopsy. miRNAs associated with disease status were identified by univariate analysis and then combined into signatures by multivariate logistic regression models. By combining 16 miRNAs differentially expressed in the T vs. HD comparison, 26 signatures were also able to significantly discriminate T from B disease. Seven of them, involving 5 specific miRNAs (miR-625, miR-423-5p, miR-370-3p, miR-181c, and miR-301b), were statistically validated in the testing set. Among the 7 signatures, the discriminatory performances of 5 were confirmed in the prospective BABE Cohort. This study identified 5 circulating miRNAs that, properly combined, distinguish malignant from benign breast disease in women with a high likelihood of malignancy. |
format | Online Article Text |
id | pubmed-8391908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83919082021-08-28 Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended Giussani, Marta Ciniselli, Chiara Maura De Cecco, Loris Lecchi, Mara Dugo, Matteo Gargiuli, Chiara Mariancini, Andrea Mancinelli, Elisa Cosentino, Giulia Veneroni, Silvia Paolini, Biagio Orlandi, Rosaria Gennaro, Massimiliano Iorio, Marilena Valeria Depretto, Catherine Ferranti, Claudio Sozzi, Gabriella Sensi, Marialuisa Colombo, Mario Paolo Scaperrotta, Gianfranco Tagliabue, Elda Verderio, Paolo Cancers (Basel) Article SIMPLE SUMMARY: In population-based screens, tissue biopsy remains the standard practice for women with imaging that suggests breast cancer. We examined circulating microRNAs as minimally invasive diagnostic biomarkers to discriminate malignant from benign breast lesions. A retrospective cohort of plasma samples divided into training and testing sets and a prospective cohort of women with suspicious imaging findings who underwent tissue biopsy were investigated through a global microRNA profile by OpenArray. Seven signatures, involving 5 specific miRNAs (miR-625, miR-423-5p, miR-370-3p, miR-181c, and miR-301b), were identified and validated in the testing set. Among the 7 signatures, the discriminatory performances of 5 of them were confirmed in the prospective cohort. ABSTRACT: In population-based screens, tissue biopsy remains the standard practice for women with imaging that suggests breast cancer. We examined circulating microRNAs as minimally invasive diagnostic biomarkers to discriminate malignant from benign breast lesions. miRNAs were analyzed by OpenArray in a retrospective cohort of plasma samples including 100 patients with malignant (T), 89 benign disease (B), and 99 healthy donors (HD) divided into training and testing sets and a prospective cohort (BABE) of 289 women with suspicious imaging findings who underwent tissue biopsy. miRNAs associated with disease status were identified by univariate analysis and then combined into signatures by multivariate logistic regression models. By combining 16 miRNAs differentially expressed in the T vs. HD comparison, 26 signatures were also able to significantly discriminate T from B disease. Seven of them, involving 5 specific miRNAs (miR-625, miR-423-5p, miR-370-3p, miR-181c, and miR-301b), were statistically validated in the testing set. Among the 7 signatures, the discriminatory performances of 5 were confirmed in the prospective BABE Cohort. This study identified 5 circulating miRNAs that, properly combined, distinguish malignant from benign breast disease in women with a high likelihood of malignancy. MDPI 2021-08-10 /pmc/articles/PMC8391908/ /pubmed/34439180 http://dx.doi.org/10.3390/cancers13164028 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Giussani, Marta Ciniselli, Chiara Maura De Cecco, Loris Lecchi, Mara Dugo, Matteo Gargiuli, Chiara Mariancini, Andrea Mancinelli, Elisa Cosentino, Giulia Veneroni, Silvia Paolini, Biagio Orlandi, Rosaria Gennaro, Massimiliano Iorio, Marilena Valeria Depretto, Catherine Ferranti, Claudio Sozzi, Gabriella Sensi, Marialuisa Colombo, Mario Paolo Scaperrotta, Gianfranco Tagliabue, Elda Verderio, Paolo Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended |
title | Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended |
title_full | Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended |
title_fullStr | Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended |
title_full_unstemmed | Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended |
title_short | Circulating miRNAs as Novel Non-Invasive Biomarkers to Aid the Early Diagnosis of Suspicious Breast Lesions for Which Biopsy Is Recommended |
title_sort | circulating mirnas as novel non-invasive biomarkers to aid the early diagnosis of suspicious breast lesions for which biopsy is recommended |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391908/ https://www.ncbi.nlm.nih.gov/pubmed/34439180 http://dx.doi.org/10.3390/cancers13164028 |
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