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Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems

Antiretroviral drugs for the treatment of human immunodeficiency virus (HIV) and other viral infections are among the emerging contaminants considered for ecological risk assessment. These compounds have been reported to be widely distributed in water bodies and other aquatic environments, while dat...

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Autores principales: Omotola, Elizabeth Oyinkansola, Genthe, Bettina, Ndlela, Luyanda, Olatunji, Olatunde Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391970/
https://www.ncbi.nlm.nih.gov/pubmed/34444108
http://dx.doi.org/10.3390/ijerph18168358
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author Omotola, Elizabeth Oyinkansola
Genthe, Bettina
Ndlela, Luyanda
Olatunji, Olatunde Stephen
author_facet Omotola, Elizabeth Oyinkansola
Genthe, Bettina
Ndlela, Luyanda
Olatunji, Olatunde Stephen
author_sort Omotola, Elizabeth Oyinkansola
collection PubMed
description Antiretroviral drugs for the treatment of human immunodeficiency virus (HIV) and other viral infections are among the emerging contaminants considered for ecological risk assessment. These compounds have been reported to be widely distributed in water bodies and other aquatic environments, while data concerning the risk they may pose to unintended non-target species in a different ecosystem (environment) is scanty. In South Africa and other developing countries, lamivudine is one of the common antiretrovirals applied. Despite this, little is known about its environmental impacts as an emerging contaminant. The present study employed a battery of ecotoxicity bioassays to assess the environmental threat lamivudine poses to aquatic fauna and flora. Daphnia magna (filter feeders), the Ames bacterial mutagenicity test, Lactuca sativa (lettuce) germination test, and the Allium cepa root tip assay were conducted, testing lamivudine at two concentrations (10 and 100 µg/L), with environmental relevance. The Daphnia magna toxicity test revealed a statistically significant response (p << 0.05) with a mortality rate of 85% on exposure to 100 µg/L lamivudine in freshwater, which increased to 100% at 48-h exposure. At lower concentrations of 10 µg/L lamivudine, 90% and 55% survival rates were observed at 24 h and 48 h, respectively. No potential mutagenic effects were observed from the Ames test at both concentrations of lamivudine. Allium cepa bioassays revealed a noticeable adverse impact on the root lengths on exposure to 100 µg/L lamivudine. This impact was further investigated through microscopic examination, revealing some chromosomal aberration in the exposed Allium cepa root tips. The Lactuca sativa bioassay showed a slight adverse impact on both the germination rate of the seeds and their respective hypocotyl lengths compared to the control. Overall, this indicates that lamivudine poses an ecological health risk at different trophic levels, to both flora and fauna, at concentrations previously found in the environment.
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spelling pubmed-83919702021-08-28 Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems Omotola, Elizabeth Oyinkansola Genthe, Bettina Ndlela, Luyanda Olatunji, Olatunde Stephen Int J Environ Res Public Health Article Antiretroviral drugs for the treatment of human immunodeficiency virus (HIV) and other viral infections are among the emerging contaminants considered for ecological risk assessment. These compounds have been reported to be widely distributed in water bodies and other aquatic environments, while data concerning the risk they may pose to unintended non-target species in a different ecosystem (environment) is scanty. In South Africa and other developing countries, lamivudine is one of the common antiretrovirals applied. Despite this, little is known about its environmental impacts as an emerging contaminant. The present study employed a battery of ecotoxicity bioassays to assess the environmental threat lamivudine poses to aquatic fauna and flora. Daphnia magna (filter feeders), the Ames bacterial mutagenicity test, Lactuca sativa (lettuce) germination test, and the Allium cepa root tip assay were conducted, testing lamivudine at two concentrations (10 and 100 µg/L), with environmental relevance. The Daphnia magna toxicity test revealed a statistically significant response (p << 0.05) with a mortality rate of 85% on exposure to 100 µg/L lamivudine in freshwater, which increased to 100% at 48-h exposure. At lower concentrations of 10 µg/L lamivudine, 90% and 55% survival rates were observed at 24 h and 48 h, respectively. No potential mutagenic effects were observed from the Ames test at both concentrations of lamivudine. Allium cepa bioassays revealed a noticeable adverse impact on the root lengths on exposure to 100 µg/L lamivudine. This impact was further investigated through microscopic examination, revealing some chromosomal aberration in the exposed Allium cepa root tips. The Lactuca sativa bioassay showed a slight adverse impact on both the germination rate of the seeds and their respective hypocotyl lengths compared to the control. Overall, this indicates that lamivudine poses an ecological health risk at different trophic levels, to both flora and fauna, at concentrations previously found in the environment. MDPI 2021-08-06 /pmc/articles/PMC8391970/ /pubmed/34444108 http://dx.doi.org/10.3390/ijerph18168358 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Omotola, Elizabeth Oyinkansola
Genthe, Bettina
Ndlela, Luyanda
Olatunji, Olatunde Stephen
Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems
title Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems
title_full Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems
title_fullStr Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems
title_full_unstemmed Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems
title_short Environmental Risk Characterization of an Antiretroviral (ARV) Lamivudine in Ecosystems
title_sort environmental risk characterization of an antiretroviral (arv) lamivudine in ecosystems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391970/
https://www.ncbi.nlm.nih.gov/pubmed/34444108
http://dx.doi.org/10.3390/ijerph18168358
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