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Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body

Monitoring exhaled breath is a safe, noninvasive method for determining the health status of the human body. Most of the components in our exhaled breath can act as health biomarkers, and they help in providing information about various diseases. Nitric oxide (NO) is one such important biomarker in...

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Autores principales: Maurya, Muni Raj, Onthath, Haseena, Morsy, Hagar, Riyaz, Najam-US-Sahar, Ibrahim, Muna, Ahmed, Alaa Elsafi, Abuznad, Raghad, Alruwaili, Aeshah, Alsaedi, Fatimatulzahraa, Kasak, Peter, Sadasivuni, Kishor Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391997/
https://www.ncbi.nlm.nih.gov/pubmed/34442192
http://dx.doi.org/10.3390/healthcare9081055
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author Maurya, Muni Raj
Onthath, Haseena
Morsy, Hagar
Riyaz, Najam-US-Sahar
Ibrahim, Muna
Ahmed, Alaa Elsafi
Abuznad, Raghad
Alruwaili, Aeshah
Alsaedi, Fatimatulzahraa
Kasak, Peter
Sadasivuni, Kishor Kumar
author_facet Maurya, Muni Raj
Onthath, Haseena
Morsy, Hagar
Riyaz, Najam-US-Sahar
Ibrahim, Muna
Ahmed, Alaa Elsafi
Abuznad, Raghad
Alruwaili, Aeshah
Alsaedi, Fatimatulzahraa
Kasak, Peter
Sadasivuni, Kishor Kumar
author_sort Maurya, Muni Raj
collection PubMed
description Monitoring exhaled breath is a safe, noninvasive method for determining the health status of the human body. Most of the components in our exhaled breath can act as health biomarkers, and they help in providing information about various diseases. Nitric oxide (NO) is one such important biomarker in exhaled breath that indicates oxidative stress in our body. This work presents a simple and noninvasive quantitative analysis approach for detecting NO from exhaled breath. The sensing is based on the colorimetric assisted detection of NO by m-Cresol Purple, Bromophenol Blue, and Alizaringelb dye. The sensing performance of the dye was analyzed by ultraviolet–visible (UV–Vis) spectroscopy. The study covers various sampling conditions like the pH effect, temperature effect, concentration effect, and selective nature of the dye. The m-Cresol Purple dye exhibited a high sensitivity towards NO with a detection limit of ~0.082 ppm in the linear range of 0.002–0.5 ppm. Moreover, the dye apprehended a high degree of selectivity towards other biocompounds present in the breath, and no possible interfering cross-reaction from these species was observed. The dye offered a high sensitivity, selectivity, fast response, and stability, which benchmark its potential for NO sensing. Further, m-Cresol Purple dye is suitable for NO sensing from the exhaled breath and can assist in quantifying oxidative stress levels in the body for the possible detection of COVID-19.
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spelling pubmed-83919972021-08-28 Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body Maurya, Muni Raj Onthath, Haseena Morsy, Hagar Riyaz, Najam-US-Sahar Ibrahim, Muna Ahmed, Alaa Elsafi Abuznad, Raghad Alruwaili, Aeshah Alsaedi, Fatimatulzahraa Kasak, Peter Sadasivuni, Kishor Kumar Healthcare (Basel) Article Monitoring exhaled breath is a safe, noninvasive method for determining the health status of the human body. Most of the components in our exhaled breath can act as health biomarkers, and they help in providing information about various diseases. Nitric oxide (NO) is one such important biomarker in exhaled breath that indicates oxidative stress in our body. This work presents a simple and noninvasive quantitative analysis approach for detecting NO from exhaled breath. The sensing is based on the colorimetric assisted detection of NO by m-Cresol Purple, Bromophenol Blue, and Alizaringelb dye. The sensing performance of the dye was analyzed by ultraviolet–visible (UV–Vis) spectroscopy. The study covers various sampling conditions like the pH effect, temperature effect, concentration effect, and selective nature of the dye. The m-Cresol Purple dye exhibited a high sensitivity towards NO with a detection limit of ~0.082 ppm in the linear range of 0.002–0.5 ppm. Moreover, the dye apprehended a high degree of selectivity towards other biocompounds present in the breath, and no possible interfering cross-reaction from these species was observed. The dye offered a high sensitivity, selectivity, fast response, and stability, which benchmark its potential for NO sensing. Further, m-Cresol Purple dye is suitable for NO sensing from the exhaled breath and can assist in quantifying oxidative stress levels in the body for the possible detection of COVID-19. MDPI 2021-08-17 /pmc/articles/PMC8391997/ /pubmed/34442192 http://dx.doi.org/10.3390/healthcare9081055 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maurya, Muni Raj
Onthath, Haseena
Morsy, Hagar
Riyaz, Najam-US-Sahar
Ibrahim, Muna
Ahmed, Alaa Elsafi
Abuznad, Raghad
Alruwaili, Aeshah
Alsaedi, Fatimatulzahraa
Kasak, Peter
Sadasivuni, Kishor Kumar
Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body
title Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body
title_full Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body
title_fullStr Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body
title_full_unstemmed Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body
title_short Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body
title_sort colorimetry-based detection of nitric oxide from exhaled breath for quantification of oxidative stress in human body
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391997/
https://www.ncbi.nlm.nih.gov/pubmed/34442192
http://dx.doi.org/10.3390/healthcare9081055
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