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Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body
Monitoring exhaled breath is a safe, noninvasive method for determining the health status of the human body. Most of the components in our exhaled breath can act as health biomarkers, and they help in providing information about various diseases. Nitric oxide (NO) is one such important biomarker in...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391997/ https://www.ncbi.nlm.nih.gov/pubmed/34442192 http://dx.doi.org/10.3390/healthcare9081055 |
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author | Maurya, Muni Raj Onthath, Haseena Morsy, Hagar Riyaz, Najam-US-Sahar Ibrahim, Muna Ahmed, Alaa Elsafi Abuznad, Raghad Alruwaili, Aeshah Alsaedi, Fatimatulzahraa Kasak, Peter Sadasivuni, Kishor Kumar |
author_facet | Maurya, Muni Raj Onthath, Haseena Morsy, Hagar Riyaz, Najam-US-Sahar Ibrahim, Muna Ahmed, Alaa Elsafi Abuznad, Raghad Alruwaili, Aeshah Alsaedi, Fatimatulzahraa Kasak, Peter Sadasivuni, Kishor Kumar |
author_sort | Maurya, Muni Raj |
collection | PubMed |
description | Monitoring exhaled breath is a safe, noninvasive method for determining the health status of the human body. Most of the components in our exhaled breath can act as health biomarkers, and they help in providing information about various diseases. Nitric oxide (NO) is one such important biomarker in exhaled breath that indicates oxidative stress in our body. This work presents a simple and noninvasive quantitative analysis approach for detecting NO from exhaled breath. The sensing is based on the colorimetric assisted detection of NO by m-Cresol Purple, Bromophenol Blue, and Alizaringelb dye. The sensing performance of the dye was analyzed by ultraviolet–visible (UV–Vis) spectroscopy. The study covers various sampling conditions like the pH effect, temperature effect, concentration effect, and selective nature of the dye. The m-Cresol Purple dye exhibited a high sensitivity towards NO with a detection limit of ~0.082 ppm in the linear range of 0.002–0.5 ppm. Moreover, the dye apprehended a high degree of selectivity towards other biocompounds present in the breath, and no possible interfering cross-reaction from these species was observed. The dye offered a high sensitivity, selectivity, fast response, and stability, which benchmark its potential for NO sensing. Further, m-Cresol Purple dye is suitable for NO sensing from the exhaled breath and can assist in quantifying oxidative stress levels in the body for the possible detection of COVID-19. |
format | Online Article Text |
id | pubmed-8391997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83919972021-08-28 Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body Maurya, Muni Raj Onthath, Haseena Morsy, Hagar Riyaz, Najam-US-Sahar Ibrahim, Muna Ahmed, Alaa Elsafi Abuznad, Raghad Alruwaili, Aeshah Alsaedi, Fatimatulzahraa Kasak, Peter Sadasivuni, Kishor Kumar Healthcare (Basel) Article Monitoring exhaled breath is a safe, noninvasive method for determining the health status of the human body. Most of the components in our exhaled breath can act as health biomarkers, and they help in providing information about various diseases. Nitric oxide (NO) is one such important biomarker in exhaled breath that indicates oxidative stress in our body. This work presents a simple and noninvasive quantitative analysis approach for detecting NO from exhaled breath. The sensing is based on the colorimetric assisted detection of NO by m-Cresol Purple, Bromophenol Blue, and Alizaringelb dye. The sensing performance of the dye was analyzed by ultraviolet–visible (UV–Vis) spectroscopy. The study covers various sampling conditions like the pH effect, temperature effect, concentration effect, and selective nature of the dye. The m-Cresol Purple dye exhibited a high sensitivity towards NO with a detection limit of ~0.082 ppm in the linear range of 0.002–0.5 ppm. Moreover, the dye apprehended a high degree of selectivity towards other biocompounds present in the breath, and no possible interfering cross-reaction from these species was observed. The dye offered a high sensitivity, selectivity, fast response, and stability, which benchmark its potential for NO sensing. Further, m-Cresol Purple dye is suitable for NO sensing from the exhaled breath and can assist in quantifying oxidative stress levels in the body for the possible detection of COVID-19. MDPI 2021-08-17 /pmc/articles/PMC8391997/ /pubmed/34442192 http://dx.doi.org/10.3390/healthcare9081055 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Maurya, Muni Raj Onthath, Haseena Morsy, Hagar Riyaz, Najam-US-Sahar Ibrahim, Muna Ahmed, Alaa Elsafi Abuznad, Raghad Alruwaili, Aeshah Alsaedi, Fatimatulzahraa Kasak, Peter Sadasivuni, Kishor Kumar Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body |
title | Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body |
title_full | Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body |
title_fullStr | Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body |
title_full_unstemmed | Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body |
title_short | Colorimetry-Based Detection of Nitric Oxide from Exhaled Breath for Quantification of Oxidative Stress in Human Body |
title_sort | colorimetry-based detection of nitric oxide from exhaled breath for quantification of oxidative stress in human body |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391997/ https://www.ncbi.nlm.nih.gov/pubmed/34442192 http://dx.doi.org/10.3390/healthcare9081055 |
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