Cargando…

Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment

Macrophage polarization and infiltration to the tumor microenvironment (TME) is a critical determining factor for tumor progression. Macrophages are polarized into two states—M1 (pro-inflammatory, anti-tumorigenic and stimulated by LPS or IFN-γ) and M2 (anti-inflammatory pro-tumorigenic and stimulat...

Descripción completa

Detalles Bibliográficos
Autores principales: Arora, Shweta, Singh, Prithvi, Ahmad, Shaniya, Ahmad, Tanveer, Dohare, Ravins, Almatroodi, Saleh A., Alrumaihi, Faris, Rahmani, Arshad Husain, Syed, Mansoor Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392240/
https://www.ncbi.nlm.nih.gov/pubmed/34440860
http://dx.doi.org/10.3390/cells10082091
_version_ 1783743454909562880
author Arora, Shweta
Singh, Prithvi
Ahmad, Shaniya
Ahmad, Tanveer
Dohare, Ravins
Almatroodi, Saleh A.
Alrumaihi, Faris
Rahmani, Arshad Husain
Syed, Mansoor Ali
author_facet Arora, Shweta
Singh, Prithvi
Ahmad, Shaniya
Ahmad, Tanveer
Dohare, Ravins
Almatroodi, Saleh A.
Alrumaihi, Faris
Rahmani, Arshad Husain
Syed, Mansoor Ali
author_sort Arora, Shweta
collection PubMed
description Macrophage polarization and infiltration to the tumor microenvironment (TME) is a critical determining factor for tumor progression. Macrophages are polarized into two states—M1 (pro-inflammatory, anti-tumorigenic and stimulated by LPS or IFN-γ) and M2 (anti-inflammatory pro-tumorigenic and stimulated by IL-4) phenotypes. Specifically, M2 macrophages enhance tumor cell growth and survival. Recent evidences suggest the pivotal role of microRNAs in macrophage polarization during the development of Non-small cell lung cancer (NSCLC), thus proposing a new therapeutic option to target lung cancer. In silico analysis determined cogent upregulation of KLF4, downregulation of IL-1β and miR-34a-5p in NSCLC tissues, consequently worsening the overall survival of NSCLC patients. We observed a significant association of KLF4 with macrophage infiltration and polarization in NSCLC. We found that KLF4 is critically implicated in M2 polarization of macrophages, which, in turn, promotes tumorigenesis. KLF4 expression correlated with miR-34a-5p and IL-1β in a feed-forward loop (FFL), both of which are implicated in immune regulation. Mechanistic overexpression of miR-34a-5p in macrophages (IL-4 stimulated) inhibits KLF4, along with downregulation of ARG1, REL-1MB (M2 macrophage specific markers), and upregulation of IL-1β, IL-6, (M1 macrophage specific markers), demonstrating macrophage polarization switch from M2 to M1 phenotype. Moreover, co-culture of these macrophages with NSCLC cells reduces their proliferation, wound healing, clonogenic capacity and enhanced NO-mediated apoptosis. Further, transfection of miR-34a-5p in NSCLC cells, also degrades KLF4, but enhances the expression of KLF4 regulated genes—IL-1β, IL-6 (pro-inflammatory mediators), which is further enhanced upon co-culture with IL-4 stimulated macrophages. Additionally, we observed a significant increase in i-NOS/NO content upon co-culture, suggesting polarization reversion of macrophages from M2 to M1, and eventually leading to anti-tumor effects. Our findings thus show a significant role of KLF4 in tumorigenesis and TAM polarization of NSCLC. However, miR-34a-5p mediated targeting of these molecular networks will provide a better therapeutic intervention for NSCLC.
format Online
Article
Text
id pubmed-8392240
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83922402021-08-28 Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment Arora, Shweta Singh, Prithvi Ahmad, Shaniya Ahmad, Tanveer Dohare, Ravins Almatroodi, Saleh A. Alrumaihi, Faris Rahmani, Arshad Husain Syed, Mansoor Ali Cells Article Macrophage polarization and infiltration to the tumor microenvironment (TME) is a critical determining factor for tumor progression. Macrophages are polarized into two states—M1 (pro-inflammatory, anti-tumorigenic and stimulated by LPS or IFN-γ) and M2 (anti-inflammatory pro-tumorigenic and stimulated by IL-4) phenotypes. Specifically, M2 macrophages enhance tumor cell growth and survival. Recent evidences suggest the pivotal role of microRNAs in macrophage polarization during the development of Non-small cell lung cancer (NSCLC), thus proposing a new therapeutic option to target lung cancer. In silico analysis determined cogent upregulation of KLF4, downregulation of IL-1β and miR-34a-5p in NSCLC tissues, consequently worsening the overall survival of NSCLC patients. We observed a significant association of KLF4 with macrophage infiltration and polarization in NSCLC. We found that KLF4 is critically implicated in M2 polarization of macrophages, which, in turn, promotes tumorigenesis. KLF4 expression correlated with miR-34a-5p and IL-1β in a feed-forward loop (FFL), both of which are implicated in immune regulation. Mechanistic overexpression of miR-34a-5p in macrophages (IL-4 stimulated) inhibits KLF4, along with downregulation of ARG1, REL-1MB (M2 macrophage specific markers), and upregulation of IL-1β, IL-6, (M1 macrophage specific markers), demonstrating macrophage polarization switch from M2 to M1 phenotype. Moreover, co-culture of these macrophages with NSCLC cells reduces their proliferation, wound healing, clonogenic capacity and enhanced NO-mediated apoptosis. Further, transfection of miR-34a-5p in NSCLC cells, also degrades KLF4, but enhances the expression of KLF4 regulated genes—IL-1β, IL-6 (pro-inflammatory mediators), which is further enhanced upon co-culture with IL-4 stimulated macrophages. Additionally, we observed a significant increase in i-NOS/NO content upon co-culture, suggesting polarization reversion of macrophages from M2 to M1, and eventually leading to anti-tumor effects. Our findings thus show a significant role of KLF4 in tumorigenesis and TAM polarization of NSCLC. However, miR-34a-5p mediated targeting of these molecular networks will provide a better therapeutic intervention for NSCLC. MDPI 2021-08-14 /pmc/articles/PMC8392240/ /pubmed/34440860 http://dx.doi.org/10.3390/cells10082091 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arora, Shweta
Singh, Prithvi
Ahmad, Shaniya
Ahmad, Tanveer
Dohare, Ravins
Almatroodi, Saleh A.
Alrumaihi, Faris
Rahmani, Arshad Husain
Syed, Mansoor Ali
Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment
title Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment
title_full Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment
title_fullStr Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment
title_full_unstemmed Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment
title_short Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment
title_sort comprehensive integrative analysis reveals the association of klf4 with macrophage infiltration and polarization in lung cancer microenvironment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392240/
https://www.ncbi.nlm.nih.gov/pubmed/34440860
http://dx.doi.org/10.3390/cells10082091
work_keys_str_mv AT arorashweta comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT singhprithvi comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT ahmadshaniya comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT ahmadtanveer comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT dohareravins comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT almatroodisaleha comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT alrumaihifaris comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT rahmaniarshadhusain comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment
AT syedmansoorali comprehensiveintegrativeanalysisrevealstheassociationofklf4withmacrophageinfiltrationandpolarizationinlungcancermicroenvironment