Cargando…

Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells

Neuronal culture was used to investigate neuronal function in physiological and pathological conditions. Despite its inevitability, primary neuronal culture remained a gold standard method that requires laborious preparation, intensive training, and animal resources. To circumvent the shortfalls of...

Descripción completa

Detalles Bibliográficos
Autores principales: Soni, Abhinav, Klütsch, Diana, Hu, Xin, Houtman, Judith, Rund, Nicole, McCloskey, Asako, Mertens, Jerome, Schafer, Simon T., Amin, Hayder, Toda, Tomohisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392300/
https://www.ncbi.nlm.nih.gov/pubmed/34440662
http://dx.doi.org/10.3390/cells10081894
_version_ 1783743471137325056
author Soni, Abhinav
Klütsch, Diana
Hu, Xin
Houtman, Judith
Rund, Nicole
McCloskey, Asako
Mertens, Jerome
Schafer, Simon T.
Amin, Hayder
Toda, Tomohisa
author_facet Soni, Abhinav
Klütsch, Diana
Hu, Xin
Houtman, Judith
Rund, Nicole
McCloskey, Asako
Mertens, Jerome
Schafer, Simon T.
Amin, Hayder
Toda, Tomohisa
author_sort Soni, Abhinav
collection PubMed
description Neuronal culture was used to investigate neuronal function in physiological and pathological conditions. Despite its inevitability, primary neuronal culture remained a gold standard method that requires laborious preparation, intensive training, and animal resources. To circumvent the shortfalls of primary neuronal preparations and efficiently give rise to functional neurons, we combine a neural stem cell culture method with a direct cell type-conversion approach. The lucidity of this method enables the efficient preparation of functional neurons from mouse neural progenitor cells on demand. We demonstrate that induced neurons (NPC-iNs) by this method make synaptic connections, elicit neuronal activity-dependent cellular responses, and develop functional neuronal networks. This method will provide a concise platform for functional neuronal assessments. This indeed offers a perspective for using these characterized neuronal networks for investigating plasticity mechanisms, drug screening assays, and probing the molecular and biophysical basis of neurodevelopmental and neurodegenerative diseases.
format Online
Article
Text
id pubmed-8392300
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83923002021-08-28 Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells Soni, Abhinav Klütsch, Diana Hu, Xin Houtman, Judith Rund, Nicole McCloskey, Asako Mertens, Jerome Schafer, Simon T. Amin, Hayder Toda, Tomohisa Cells Article Neuronal culture was used to investigate neuronal function in physiological and pathological conditions. Despite its inevitability, primary neuronal culture remained a gold standard method that requires laborious preparation, intensive training, and animal resources. To circumvent the shortfalls of primary neuronal preparations and efficiently give rise to functional neurons, we combine a neural stem cell culture method with a direct cell type-conversion approach. The lucidity of this method enables the efficient preparation of functional neurons from mouse neural progenitor cells on demand. We demonstrate that induced neurons (NPC-iNs) by this method make synaptic connections, elicit neuronal activity-dependent cellular responses, and develop functional neuronal networks. This method will provide a concise platform for functional neuronal assessments. This indeed offers a perspective for using these characterized neuronal networks for investigating plasticity mechanisms, drug screening assays, and probing the molecular and biophysical basis of neurodevelopmental and neurodegenerative diseases. MDPI 2021-07-26 /pmc/articles/PMC8392300/ /pubmed/34440662 http://dx.doi.org/10.3390/cells10081894 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soni, Abhinav
Klütsch, Diana
Hu, Xin
Houtman, Judith
Rund, Nicole
McCloskey, Asako
Mertens, Jerome
Schafer, Simon T.
Amin, Hayder
Toda, Tomohisa
Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells
title Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells
title_full Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells
title_fullStr Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells
title_full_unstemmed Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells
title_short Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells
title_sort improved method for efficient generation of functional neurons from murine neural progenitor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392300/
https://www.ncbi.nlm.nih.gov/pubmed/34440662
http://dx.doi.org/10.3390/cells10081894
work_keys_str_mv AT soniabhinav improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT klutschdiana improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT huxin improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT houtmanjudith improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT rundnicole improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT mccloskeyasako improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT mertensjerome improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT schafersimont improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT aminhayder improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells
AT todatomohisa improvedmethodforefficientgenerationoffunctionalneuronsfrommurineneuralprogenitorcells