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cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor

Changes of circulating free plasma DNA (cfDNA) are associated with different types of tissue injury, including those induced by intensive aerobic and anaerobic exercises. Observed changes are dependent from induced inflammation, and thus it may be a potential marker for athletic overtraining. We aim...

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Autores principales: Humińska-Lisowska, Kinga, Mieszkowski, Jan, Kochanowicz, Andrzej, Stankiewicz, Błażej, Niespodziński, Bartłomiej, Brzezińska, Paulina, Ficek, Krzysztof, Kemerytė-Ivanauskienė, Eglė, Cięszczyk, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392318/
https://www.ncbi.nlm.nih.gov/pubmed/34440412
http://dx.doi.org/10.3390/genes12081238
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author Humińska-Lisowska, Kinga
Mieszkowski, Jan
Kochanowicz, Andrzej
Stankiewicz, Błażej
Niespodziński, Bartłomiej
Brzezińska, Paulina
Ficek, Krzysztof
Kemerytė-Ivanauskienė, Eglė
Cięszczyk, Paweł
author_facet Humińska-Lisowska, Kinga
Mieszkowski, Jan
Kochanowicz, Andrzej
Stankiewicz, Błażej
Niespodziński, Bartłomiej
Brzezińska, Paulina
Ficek, Krzysztof
Kemerytė-Ivanauskienė, Eglė
Cięszczyk, Paweł
author_sort Humińska-Lisowska, Kinga
collection PubMed
description Changes of circulating free plasma DNA (cfDNA) are associated with different types of tissue injury, including those induced by intensive aerobic and anaerobic exercises. Observed changes are dependent from induced inflammation, and thus it may be a potential marker for athletic overtraining. We aimed to identify the response of cfDNA to different types of exercise, with association to exercise intensity as a potential marker of exercise load. Fifty volunteers (25 athletes and 25 physically active men) were assigned to the study and performed maximal aerobic (Bruce test) and anaerobic (Wingate Anaerobic Test) test. Blood samples for cfDNA analysis were collected at four time-points: before, 2–5 min after, 30 min after and 60 min after each type of maximal physical activity. The two-way ANOVA revealed a significant effect of group factor on serum cfDNA concentrations (32.15% higher concentration of cfDNA in the athletes). In turn the results of the post hoc test for the interaction of the repeated measures factor and the group showed that while the concentration of cfDNA decreased by 40.10% in the period from 30 min to 60 min after exercise in the control group, the concentration of cfDNA in the group of athletes remained at a similar level. Our analysis presents different responses depending on the intensity and duration of exercise. Our observations imply that formation of cfDNA is associated with response to physical activity but only during maximal effort.
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spelling pubmed-83923182021-08-28 cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor Humińska-Lisowska, Kinga Mieszkowski, Jan Kochanowicz, Andrzej Stankiewicz, Błażej Niespodziński, Bartłomiej Brzezińska, Paulina Ficek, Krzysztof Kemerytė-Ivanauskienė, Eglė Cięszczyk, Paweł Genes (Basel) Article Changes of circulating free plasma DNA (cfDNA) are associated with different types of tissue injury, including those induced by intensive aerobic and anaerobic exercises. Observed changes are dependent from induced inflammation, and thus it may be a potential marker for athletic overtraining. We aimed to identify the response of cfDNA to different types of exercise, with association to exercise intensity as a potential marker of exercise load. Fifty volunteers (25 athletes and 25 physically active men) were assigned to the study and performed maximal aerobic (Bruce test) and anaerobic (Wingate Anaerobic Test) test. Blood samples for cfDNA analysis were collected at four time-points: before, 2–5 min after, 30 min after and 60 min after each type of maximal physical activity. The two-way ANOVA revealed a significant effect of group factor on serum cfDNA concentrations (32.15% higher concentration of cfDNA in the athletes). In turn the results of the post hoc test for the interaction of the repeated measures factor and the group showed that while the concentration of cfDNA decreased by 40.10% in the period from 30 min to 60 min after exercise in the control group, the concentration of cfDNA in the group of athletes remained at a similar level. Our analysis presents different responses depending on the intensity and duration of exercise. Our observations imply that formation of cfDNA is associated with response to physical activity but only during maximal effort. MDPI 2021-08-12 /pmc/articles/PMC8392318/ /pubmed/34440412 http://dx.doi.org/10.3390/genes12081238 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Humińska-Lisowska, Kinga
Mieszkowski, Jan
Kochanowicz, Andrzej
Stankiewicz, Błażej
Niespodziński, Bartłomiej
Brzezińska, Paulina
Ficek, Krzysztof
Kemerytė-Ivanauskienė, Eglė
Cięszczyk, Paweł
cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor
title cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor
title_full cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor
title_fullStr cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor
title_full_unstemmed cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor
title_short cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor
title_sort cfdna changes in maximal exercises as a sport adaptation predictor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392318/
https://www.ncbi.nlm.nih.gov/pubmed/34440412
http://dx.doi.org/10.3390/genes12081238
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