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Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer

Apolipoprotein A1 (APOA1) is a potential biomarker because of its variable concentration in different types of cancers. The current study is the first of its kind to evaluate the association between the APOA1 genotypes of −75 G/A and +83 C/T in tandem with the APOA1 protein expression in urine sampl...

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Autores principales: Magray, Javid A., Pandith, Arshad A., Qasim, Iqbal, Khateeb, Muzzain, Hamid, Arif, Koul, Aabid, Shah, Zafar A., Baba, Shahid M., Mansoor, Sheikh, Charifi, Wafa, Ahmad, Ajaz, Wani, Mohammad S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392831/
https://www.ncbi.nlm.nih.gov/pubmed/34440141
http://dx.doi.org/10.3390/biomedicines9080938
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author Magray, Javid A.
Pandith, Arshad A.
Qasim, Iqbal
Khateeb, Muzzain
Hamid, Arif
Koul, Aabid
Shah, Zafar A.
Baba, Shahid M.
Mansoor, Sheikh
Charifi, Wafa
Ahmad, Ajaz
Wani, Mohammad S.
author_facet Magray, Javid A.
Pandith, Arshad A.
Qasim, Iqbal
Khateeb, Muzzain
Hamid, Arif
Koul, Aabid
Shah, Zafar A.
Baba, Shahid M.
Mansoor, Sheikh
Charifi, Wafa
Ahmad, Ajaz
Wani, Mohammad S.
author_sort Magray, Javid A.
collection PubMed
description Apolipoprotein A1 (APOA1) is a potential biomarker because of its variable concentration in different types of cancers. The current study is the first of its kind to evaluate the association between the APOA1 genotypes of −75 G/A and +83 C/T in tandem with the APOA1 protein expression in urine samples to find out the risk and potential relationship for differentially expressed urinary proteins and APOA1 genotypes. The study included 108 cases of bladder tumors and 150 healthy controls that were frequency matched to cases with respect to age, sex, and smoking status. Genotyping was performed using PCR-RFLP and the urinary expression of the APOA1 protein was done using ELISA. Bladder tumor cases were significantly associated with the APOA1 −75 AA genotype (p < 0.05), while the APOA1 +83 C/T heterozygotes showed an association with cases (p < 0.05). The overall distribution of the different haplotypes showed a marked difference between the cases and controls in GT when compared with the wild type GC (p < 0.03). Bladder tumor cases that carried the variant genotype APOA1 −75AA were found more (70.0%) with a higher expression (≥20 ng/mL)of the APOA1 urinary protein and differed significantly against wild type GG (p = 0.03). Again, in low grade bladder tumors, urinary APOA1 protein was exhibited significantly more (52.4% vs. 15.4% high grade) with a higher expression (≥20 ng), while high grade tumor cases (84.6% vs. 47.5% low grade) showed a lower APOA1 expression (<20 ng/mL) (O.R = 6.08, p = 0.002). A strong association was observed between APOA1 −75G/A and risk for bladder tumor and its relation to urinary protein expression, which substantiates its possible role as a marker for the risk assessment of the disease and as a promising diagnostic marker for different grades of malignant bladder tumors.
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spelling pubmed-83928312021-08-28 Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer Magray, Javid A. Pandith, Arshad A. Qasim, Iqbal Khateeb, Muzzain Hamid, Arif Koul, Aabid Shah, Zafar A. Baba, Shahid M. Mansoor, Sheikh Charifi, Wafa Ahmad, Ajaz Wani, Mohammad S. Biomedicines Article Apolipoprotein A1 (APOA1) is a potential biomarker because of its variable concentration in different types of cancers. The current study is the first of its kind to evaluate the association between the APOA1 genotypes of −75 G/A and +83 C/T in tandem with the APOA1 protein expression in urine samples to find out the risk and potential relationship for differentially expressed urinary proteins and APOA1 genotypes. The study included 108 cases of bladder tumors and 150 healthy controls that were frequency matched to cases with respect to age, sex, and smoking status. Genotyping was performed using PCR-RFLP and the urinary expression of the APOA1 protein was done using ELISA. Bladder tumor cases were significantly associated with the APOA1 −75 AA genotype (p < 0.05), while the APOA1 +83 C/T heterozygotes showed an association with cases (p < 0.05). The overall distribution of the different haplotypes showed a marked difference between the cases and controls in GT when compared with the wild type GC (p < 0.03). Bladder tumor cases that carried the variant genotype APOA1 −75AA were found more (70.0%) with a higher expression (≥20 ng/mL)of the APOA1 urinary protein and differed significantly against wild type GG (p = 0.03). Again, in low grade bladder tumors, urinary APOA1 protein was exhibited significantly more (52.4% vs. 15.4% high grade) with a higher expression (≥20 ng), while high grade tumor cases (84.6% vs. 47.5% low grade) showed a lower APOA1 expression (<20 ng/mL) (O.R = 6.08, p = 0.002). A strong association was observed between APOA1 −75G/A and risk for bladder tumor and its relation to urinary protein expression, which substantiates its possible role as a marker for the risk assessment of the disease and as a promising diagnostic marker for different grades of malignant bladder tumors. MDPI 2021-08-02 /pmc/articles/PMC8392831/ /pubmed/34440141 http://dx.doi.org/10.3390/biomedicines9080938 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Magray, Javid A.
Pandith, Arshad A.
Qasim, Iqbal
Khateeb, Muzzain
Hamid, Arif
Koul, Aabid
Shah, Zafar A.
Baba, Shahid M.
Mansoor, Sheikh
Charifi, Wafa
Ahmad, Ajaz
Wani, Mohammad S.
Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer
title Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer
title_full Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer
title_fullStr Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer
title_full_unstemmed Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer
title_short Significant Implications of APOA1 Gene Sequence Variations and Its Protein Expression in Bladder Cancer
title_sort significant implications of apoa1 gene sequence variations and its protein expression in bladder cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392831/
https://www.ncbi.nlm.nih.gov/pubmed/34440141
http://dx.doi.org/10.3390/biomedicines9080938
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