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Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM

The CRISPR/Cas9 system has been widely used for gene editing in zebrafish. However, the required NGG protospacer adjacent motif (PAM) of Streptococcus pyogenes Cas9 (SpCas9) notably restricts the editable range of the zebrafish genome. Recently, Cas9 from S. canis (ScCas9), which has a more relaxed...

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Detalles Bibliográficos
Autores principales:	Liu, Yunxing, Liang, Fang, Dong, Zijiong, Li, Song, Ye, Jianmin, Qin, Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392876/ https://www.ncbi.nlm.nih.gov/pubmed/34440868 http://dx.doi.org/10.3390/cells10082099

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