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Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering

The purpose of this study was to investigate, in vitro and in vivo, the suitability of chitosan (CHS) scaffolds produced by the net-shape-nonwoven (NSN) technology, for use as bone graft substitutes in a critical-size femoral bone defect in rats. For in vitro investigations, scaffolds made of CHS, m...

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Autores principales: Zwingenberger, Bruno, Vater, Corina, Bell, Roland L., Bolte, Julia, Mehnert, Elisabeth, Brünler, Ronny, Aibibu, Dilbar, Zwingenberger, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393398/
https://www.ncbi.nlm.nih.gov/pubmed/34440219
http://dx.doi.org/10.3390/biomedicines9081015
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author Zwingenberger, Bruno
Vater, Corina
Bell, Roland L.
Bolte, Julia
Mehnert, Elisabeth
Brünler, Ronny
Aibibu, Dilbar
Zwingenberger, Stefan
author_facet Zwingenberger, Bruno
Vater, Corina
Bell, Roland L.
Bolte, Julia
Mehnert, Elisabeth
Brünler, Ronny
Aibibu, Dilbar
Zwingenberger, Stefan
author_sort Zwingenberger, Bruno
collection PubMed
description The purpose of this study was to investigate, in vitro and in vivo, the suitability of chitosan (CHS) scaffolds produced by the net-shape-nonwoven (NSN) technology, for use as bone graft substitutes in a critical-size femoral bone defect in rats. For in vitro investigations, scaffolds made of CHS, mineralized collagen (MCM), or human cancellous bone allograft (CBA) were seeded with human telomerase-immortalized mesenchymal stromal cells (hTERT-MSC), incubated for 14 days, and thereafter evaluated for proliferation and osteogenic differentiation. In vivo, CHS, MCM and CBA scaffolds were implanted into 5 mm critical-size femoral bone defects in rats. After 12 weeks, the volume of newly formed bone was determined by microcomputed tomography (µCT), while the degree of defect healing, as well as vascularization and the number of osteoblasts and osteoclasts, was evaluated histologically. In vitro, CHS scaffolds showed significantly higher osteogenic properties, whereas treatment with CHS, in vivo, led to a lower grade of bone-healing compared to CBA and MCM. While chitosan offers a completely new field of scaffold production by fibers, these scaffolds will have to be improved in the future, regarding mechanical stability and osteoconductivity.
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spelling pubmed-83933982021-08-28 Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering Zwingenberger, Bruno Vater, Corina Bell, Roland L. Bolte, Julia Mehnert, Elisabeth Brünler, Ronny Aibibu, Dilbar Zwingenberger, Stefan Biomedicines Article The purpose of this study was to investigate, in vitro and in vivo, the suitability of chitosan (CHS) scaffolds produced by the net-shape-nonwoven (NSN) technology, for use as bone graft substitutes in a critical-size femoral bone defect in rats. For in vitro investigations, scaffolds made of CHS, mineralized collagen (MCM), or human cancellous bone allograft (CBA) were seeded with human telomerase-immortalized mesenchymal stromal cells (hTERT-MSC), incubated for 14 days, and thereafter evaluated for proliferation and osteogenic differentiation. In vivo, CHS, MCM and CBA scaffolds were implanted into 5 mm critical-size femoral bone defects in rats. After 12 weeks, the volume of newly formed bone was determined by microcomputed tomography (µCT), while the degree of defect healing, as well as vascularization and the number of osteoblasts and osteoclasts, was evaluated histologically. In vitro, CHS scaffolds showed significantly higher osteogenic properties, whereas treatment with CHS, in vivo, led to a lower grade of bone-healing compared to CBA and MCM. While chitosan offers a completely new field of scaffold production by fibers, these scaffolds will have to be improved in the future, regarding mechanical stability and osteoconductivity. MDPI 2021-08-14 /pmc/articles/PMC8393398/ /pubmed/34440219 http://dx.doi.org/10.3390/biomedicines9081015 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zwingenberger, Bruno
Vater, Corina
Bell, Roland L.
Bolte, Julia
Mehnert, Elisabeth
Brünler, Ronny
Aibibu, Dilbar
Zwingenberger, Stefan
Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering
title Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering
title_full Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering
title_fullStr Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering
title_full_unstemmed Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering
title_short Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering
title_sort treatment of critical-size femoral bone defects with chitosan scaffolds produced by a novel process from textile engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393398/
https://www.ncbi.nlm.nih.gov/pubmed/34440219
http://dx.doi.org/10.3390/biomedicines9081015
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