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Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale

Chinese kale (Brassica oleracea var. alboglabra) is rich in carotenoids, and neoxanthin is one of the most important carotenoids in Chinese kale. In this study, the function of the neoxanthin synthase gene (BoaNXS) in Chinese kale was investigated. BoaNXS, which had a 699-bp coding sequence, was clo...

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Autores principales: Jian, Yue, Zhang, Chenlu, Wang, Yating, Li, Zhiqing, Chen, Jing, Zhou, Wenting, Huang, Wenli, Jiang, Min, Zheng, Hao, Li, Mengyao, Miao, Huiying, Zhang, Fen, Li, Huanxiu, Wang, Qiaomei, Sun, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393425/
https://www.ncbi.nlm.nih.gov/pubmed/34440295
http://dx.doi.org/10.3390/genes12081122
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author Jian, Yue
Zhang, Chenlu
Wang, Yating
Li, Zhiqing
Chen, Jing
Zhou, Wenting
Huang, Wenli
Jiang, Min
Zheng, Hao
Li, Mengyao
Miao, Huiying
Zhang, Fen
Li, Huanxiu
Wang, Qiaomei
Sun, Bo
author_facet Jian, Yue
Zhang, Chenlu
Wang, Yating
Li, Zhiqing
Chen, Jing
Zhou, Wenting
Huang, Wenli
Jiang, Min
Zheng, Hao
Li, Mengyao
Miao, Huiying
Zhang, Fen
Li, Huanxiu
Wang, Qiaomei
Sun, Bo
author_sort Jian, Yue
collection PubMed
description Chinese kale (Brassica oleracea var. alboglabra) is rich in carotenoids, and neoxanthin is one of the most important carotenoids in Chinese kale. In this study, the function of the neoxanthin synthase gene (BoaNXS) in Chinese kale was investigated. BoaNXS, which had a 699-bp coding sequence, was cloned from the white flower cultivar of Chinese kale and was expressed in all developmental stages and organs of Chinese kale; its expression was highest in young seeds. The subcellular localization indicated that BoaNXS was localized in the chloroplast. BoaNXS-overexpressed plants were obtained via Agrobacterium-mediated transient overexpression methodology, and the gene overexpression efficiencies ranged from 2.10- to 4.24-fold. The color in the leaves of BoaNXS-overexpressed plants changed from green to yellow-green; the content of total and individual carotenoids, such as neoxanthin, violaxanthin, and lutein, was significantly increased, and the expression levels of most carotenoid biosynthetic genes were notably increased. These findings indicated that BoaNXS is of vital importance in carotenoid biosynthesis in Chinese kale and could be used as a candidate gene for enriching the carotenoid accumulation and color of Chinese kale and other Brassica vegetables.
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spelling pubmed-83934252021-08-28 Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale Jian, Yue Zhang, Chenlu Wang, Yating Li, Zhiqing Chen, Jing Zhou, Wenting Huang, Wenli Jiang, Min Zheng, Hao Li, Mengyao Miao, Huiying Zhang, Fen Li, Huanxiu Wang, Qiaomei Sun, Bo Genes (Basel) Article Chinese kale (Brassica oleracea var. alboglabra) is rich in carotenoids, and neoxanthin is one of the most important carotenoids in Chinese kale. In this study, the function of the neoxanthin synthase gene (BoaNXS) in Chinese kale was investigated. BoaNXS, which had a 699-bp coding sequence, was cloned from the white flower cultivar of Chinese kale and was expressed in all developmental stages and organs of Chinese kale; its expression was highest in young seeds. The subcellular localization indicated that BoaNXS was localized in the chloroplast. BoaNXS-overexpressed plants were obtained via Agrobacterium-mediated transient overexpression methodology, and the gene overexpression efficiencies ranged from 2.10- to 4.24-fold. The color in the leaves of BoaNXS-overexpressed plants changed from green to yellow-green; the content of total and individual carotenoids, such as neoxanthin, violaxanthin, and lutein, was significantly increased, and the expression levels of most carotenoid biosynthetic genes were notably increased. These findings indicated that BoaNXS is of vital importance in carotenoid biosynthesis in Chinese kale and could be used as a candidate gene for enriching the carotenoid accumulation and color of Chinese kale and other Brassica vegetables. MDPI 2021-07-24 /pmc/articles/PMC8393425/ /pubmed/34440295 http://dx.doi.org/10.3390/genes12081122 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jian, Yue
Zhang, Chenlu
Wang, Yating
Li, Zhiqing
Chen, Jing
Zhou, Wenting
Huang, Wenli
Jiang, Min
Zheng, Hao
Li, Mengyao
Miao, Huiying
Zhang, Fen
Li, Huanxiu
Wang, Qiaomei
Sun, Bo
Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale
title Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale
title_full Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale
title_fullStr Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale
title_full_unstemmed Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale
title_short Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale
title_sort characterization of the role of the neoxanthin synthase gene boanxs in carotenoid biosynthesis in chinese kale
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393425/
https://www.ncbi.nlm.nih.gov/pubmed/34440295
http://dx.doi.org/10.3390/genes12081122
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