Cargando…
Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering
Cyanobacterial bloom is one of the most urgent global environmental issues, which eventually could threaten human health and safety. Sonication treatment (ST) is a potential effective method to control cyanobacteria blooms in the field. Currently, the bottleneck of extensive application of ST is the...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393532/ https://www.ncbi.nlm.nih.gov/pubmed/34436081 http://dx.doi.org/10.3390/bios11080279 |
_version_ | 1783743747439198208 |
---|---|
author | Li, Jiajin Zou, Chujun Liao, Ran Peng, Liang Wang, Hongjian Guo, Zhiming Ma, Hui |
author_facet | Li, Jiajin Zou, Chujun Liao, Ran Peng, Liang Wang, Hongjian Guo, Zhiming Ma, Hui |
author_sort | Li, Jiajin |
collection | PubMed |
description | Cyanobacterial bloom is one of the most urgent global environmental issues, which eventually could threaten human health and safety. Sonication treatment (ST) is a potential effective method to control cyanobacteria blooms in the field. Currently, the bottleneck of extensive application of ST is the difficulty to estimate the ST effect on the cyanobacterial cells and then determine suitable ST times in the field. In this study, cyanobacterial Microcystis samples sonicated at different times were first measured by a spectrophotometer to calculate the removal efficiency of Microcystis cells. Additionally, they were observed by TEM to reveal the intracellular structure changes of the cells. Then the samples were measured by an experimental setup based on polarized light scattering to measure the polarization parameters. Experimental results indicated that the polarization parameters can effectively characterize the intracellular structural changes of Microcystis cells with different ST times, which is quite consistent with the results for removal efficiency and TEM images. Further, the optimal ST time can be inferred by the polarization parameters. These results demonstrate that polarized light scattering can be a potentially powerful tool to explore suitable times for sonication treatment of cyanobacteria blooms. |
format | Online Article Text |
id | pubmed-8393532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83935322021-08-28 Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering Li, Jiajin Zou, Chujun Liao, Ran Peng, Liang Wang, Hongjian Guo, Zhiming Ma, Hui Biosensors (Basel) Article Cyanobacterial bloom is one of the most urgent global environmental issues, which eventually could threaten human health and safety. Sonication treatment (ST) is a potential effective method to control cyanobacteria blooms in the field. Currently, the bottleneck of extensive application of ST is the difficulty to estimate the ST effect on the cyanobacterial cells and then determine suitable ST times in the field. In this study, cyanobacterial Microcystis samples sonicated at different times were first measured by a spectrophotometer to calculate the removal efficiency of Microcystis cells. Additionally, they were observed by TEM to reveal the intracellular structure changes of the cells. Then the samples were measured by an experimental setup based on polarized light scattering to measure the polarization parameters. Experimental results indicated that the polarization parameters can effectively characterize the intracellular structural changes of Microcystis cells with different ST times, which is quite consistent with the results for removal efficiency and TEM images. Further, the optimal ST time can be inferred by the polarization parameters. These results demonstrate that polarized light scattering can be a potentially powerful tool to explore suitable times for sonication treatment of cyanobacteria blooms. MDPI 2021-08-17 /pmc/articles/PMC8393532/ /pubmed/34436081 http://dx.doi.org/10.3390/bios11080279 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Jiajin Zou, Chujun Liao, Ran Peng, Liang Wang, Hongjian Guo, Zhiming Ma, Hui Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering |
title | Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering |
title_full | Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering |
title_fullStr | Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering |
title_full_unstemmed | Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering |
title_short | Characterization of Intracellular Structure Changes of Microcystis under Sonication Treatment by Polarized Light Scattering |
title_sort | characterization of intracellular structure changes of microcystis under sonication treatment by polarized light scattering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393532/ https://www.ncbi.nlm.nih.gov/pubmed/34436081 http://dx.doi.org/10.3390/bios11080279 |
work_keys_str_mv | AT lijiajin characterizationofintracellularstructurechangesofmicrocystisundersonicationtreatmentbypolarizedlightscattering AT zouchujun characterizationofintracellularstructurechangesofmicrocystisundersonicationtreatmentbypolarizedlightscattering AT liaoran characterizationofintracellularstructurechangesofmicrocystisundersonicationtreatmentbypolarizedlightscattering AT pengliang characterizationofintracellularstructurechangesofmicrocystisundersonicationtreatmentbypolarizedlightscattering AT wanghongjian characterizationofintracellularstructurechangesofmicrocystisundersonicationtreatmentbypolarizedlightscattering AT guozhiming characterizationofintracellularstructurechangesofmicrocystisundersonicationtreatmentbypolarizedlightscattering AT mahui characterizationofintracellularstructurechangesofmicrocystisundersonicationtreatmentbypolarizedlightscattering |