Cargando…

Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases

Perinuclear anti-neutrophilic cytoplasmic antibodies (P-ANCA) recognize heterogeneous antigens, including myeloperoxidase (MPO), lactoferrin, elastase, cathepsin-G and bactericidal/permeability-increasing protein. Although P-ANCA have diagnostic utility in vasculitides, they may also be found in pat...

Descripción completa

Detalles Bibliográficos
Autores principales: Argyropoulou, Ourania D., Goules, Andreas V., Boutzios, Georgios, Tsirogianni, Alexandra, Sfontouris, Charalampos, Manoussakis, Menelaos N., Vlachoyiannopoulos, Panayiotis G., Tzioufas, Athanasios G., Kapsogeorgou, Efstathia K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393570/
https://www.ncbi.nlm.nih.gov/pubmed/34440897
http://dx.doi.org/10.3390/cells10082128
_version_ 1783743755333926912
author Argyropoulou, Ourania D.
Goules, Andreas V.
Boutzios, Georgios
Tsirogianni, Alexandra
Sfontouris, Charalampos
Manoussakis, Menelaos N.
Vlachoyiannopoulos, Panayiotis G.
Tzioufas, Athanasios G.
Kapsogeorgou, Efstathia K.
author_facet Argyropoulou, Ourania D.
Goules, Andreas V.
Boutzios, Georgios
Tsirogianni, Alexandra
Sfontouris, Charalampos
Manoussakis, Menelaos N.
Vlachoyiannopoulos, Panayiotis G.
Tzioufas, Athanasios G.
Kapsogeorgou, Efstathia K.
author_sort Argyropoulou, Ourania D.
collection PubMed
description Perinuclear anti-neutrophilic cytoplasmic antibodies (P-ANCA) recognize heterogeneous antigens, including myeloperoxidase (MPO), lactoferrin, elastase, cathepsin-G and bactericidal/permeability-increasing protein. Although P-ANCA have diagnostic utility in vasculitides, they may also be found in patients with various other systemic autoimmune rheumatic diseases (SARDs). Nevertheless, the clinical significance and the targets recognized by P-ANCA in such patients remain unclear. For this purpose, herein we investigated the occurrence of ANCA-related antigenic specificities in 82 P-ANCA-positive sera by multiplex ELISA, as well as their association with other autoantibodies. The P-ANCA-positive sera corresponded to patients with vasculitides (n = 24), systemic lupus erythematosus (n = 28), antiphospholipid syndrome (n = 5), Sjögren’s syndrome (n = 7), rheumatoid arthritis (n = 3), systemic scleroderma (n = 1), sarcoidosis (n = 1) and Hashimoto′s thyroiditis (n = 13). In most P-ANCA-positive patients studied (51/82, 62.3%), these autoantibodies occurred in high titers (>1:160). The analysis of P-ANCA-positive sera revealed reactivity to MPO in only 50% of patients with vasculitides, whereas it was infrequent in the other disease groups studied. Reactivity to other P-ANCA-related autoantigens was also rarely detected. Our findings support that high P-ANCA titers occur in SARD. The P-ANCA-positive staining pattern is associated with MPO specificity in vasculitides, while in other autoimmune diseases, it mostly involves unknown autoantigens.
format Online
Article
Text
id pubmed-8393570
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83935702021-08-28 Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases Argyropoulou, Ourania D. Goules, Andreas V. Boutzios, Georgios Tsirogianni, Alexandra Sfontouris, Charalampos Manoussakis, Menelaos N. Vlachoyiannopoulos, Panayiotis G. Tzioufas, Athanasios G. Kapsogeorgou, Efstathia K. Cells Article Perinuclear anti-neutrophilic cytoplasmic antibodies (P-ANCA) recognize heterogeneous antigens, including myeloperoxidase (MPO), lactoferrin, elastase, cathepsin-G and bactericidal/permeability-increasing protein. Although P-ANCA have diagnostic utility in vasculitides, they may also be found in patients with various other systemic autoimmune rheumatic diseases (SARDs). Nevertheless, the clinical significance and the targets recognized by P-ANCA in such patients remain unclear. For this purpose, herein we investigated the occurrence of ANCA-related antigenic specificities in 82 P-ANCA-positive sera by multiplex ELISA, as well as their association with other autoantibodies. The P-ANCA-positive sera corresponded to patients with vasculitides (n = 24), systemic lupus erythematosus (n = 28), antiphospholipid syndrome (n = 5), Sjögren’s syndrome (n = 7), rheumatoid arthritis (n = 3), systemic scleroderma (n = 1), sarcoidosis (n = 1) and Hashimoto′s thyroiditis (n = 13). In most P-ANCA-positive patients studied (51/82, 62.3%), these autoantibodies occurred in high titers (>1:160). The analysis of P-ANCA-positive sera revealed reactivity to MPO in only 50% of patients with vasculitides, whereas it was infrequent in the other disease groups studied. Reactivity to other P-ANCA-related autoantigens was also rarely detected. Our findings support that high P-ANCA titers occur in SARD. The P-ANCA-positive staining pattern is associated with MPO specificity in vasculitides, while in other autoimmune diseases, it mostly involves unknown autoantigens. MDPI 2021-08-19 /pmc/articles/PMC8393570/ /pubmed/34440897 http://dx.doi.org/10.3390/cells10082128 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Argyropoulou, Ourania D.
Goules, Andreas V.
Boutzios, Georgios
Tsirogianni, Alexandra
Sfontouris, Charalampos
Manoussakis, Menelaos N.
Vlachoyiannopoulos, Panayiotis G.
Tzioufas, Athanasios G.
Kapsogeorgou, Efstathia K.
Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases
title Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases
title_full Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases
title_fullStr Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases
title_full_unstemmed Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases
title_short Occurrence and Antigenic Specificity of Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA) in Systemic Autoimmune Diseases
title_sort occurrence and antigenic specificity of perinuclear anti-neutrophil cytoplasmic antibodies (p-anca) in systemic autoimmune diseases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393570/
https://www.ncbi.nlm.nih.gov/pubmed/34440897
http://dx.doi.org/10.3390/cells10082128
work_keys_str_mv AT argyropoulououraniad occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT goulesandreasv occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT boutziosgeorgios occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT tsirogiannialexandra occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT sfontourischaralampos occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT manoussakismenelaosn occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT vlachoyiannopoulospanayiotisg occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT tzioufasathanasiosg occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases
AT kapsogeorgouefstathiak occurrenceandantigenicspecificityofperinuclearantineutrophilcytoplasmicantibodiespancainsystemicautoimmunediseases