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miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1

A number of previous studies have reported that dysregulated miR-184 expression is associated with the development of cancer. The aim of the present study was to investigate the role of miR-184 in prostate cancer (PC) and the mechanism underlying its effects. Data from human tumor tissue samples wer...

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Autores principales: Tan, Gui-Geng, Xu, Chang, Zhong, Wei-Kang, Wang, Chuan-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393589/
https://www.ncbi.nlm.nih.gov/pubmed/34504608
http://dx.doi.org/10.3892/etm.2021.10597
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author Tan, Gui-Geng
Xu, Chang
Zhong, Wei-Kang
Wang, Chuan-Yun
author_facet Tan, Gui-Geng
Xu, Chang
Zhong, Wei-Kang
Wang, Chuan-Yun
author_sort Tan, Gui-Geng
collection PubMed
description A number of previous studies have reported that dysregulated miR-184 expression is associated with the development of cancer. The aim of the present study was to investigate the role of miR-184 in prostate cancer (PC) and the mechanism underlying its effects. Data from human tumor tissue samples were collected from The CEancer Genome Atlas to determine the expression levels of miR-184 and DLX1. The miR-184 mimic and pcDNA3.1-DLX1 plasmid were utilized to induce overexpression of miR-184 and DLX1 in Du145 cells, respectively. Cell Counting Kit-8, wound healing and Transwell assays were performed to examine the effects of miR-184 on the aggressiveness of PC cells. Dual-luciferase reporter gene assay was used to investigate the association between miR-184 and DLX1, and reverse transcription-quantitative PCR and western blot analyses were utilized to determine the mRNA and protein levels. miR-184 expression was found to be downregulated whereas DLX1 was upregulated in PC tissues compared with normal prostate tissues. Cell propagation, migration and invasion were all inhibited by miR-184 upregulation in Du145 cells. Dual luciferase reporter assay confirmed the association between miR-184 and DLX1. The inhibitory effect of miR-184 mimic on cell behaviors was reversed by upregulation of DLX1. These findings suggest that miR-184 plays a beneficial role in suppressing the tumorigenesis of PC by directly targeting DLX1, and it may represent a potential therapeutic strategy for PC.
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spelling pubmed-83935892021-09-08 miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1 Tan, Gui-Geng Xu, Chang Zhong, Wei-Kang Wang, Chuan-Yun Exp Ther Med Articles A number of previous studies have reported that dysregulated miR-184 expression is associated with the development of cancer. The aim of the present study was to investigate the role of miR-184 in prostate cancer (PC) and the mechanism underlying its effects. Data from human tumor tissue samples were collected from The CEancer Genome Atlas to determine the expression levels of miR-184 and DLX1. The miR-184 mimic and pcDNA3.1-DLX1 plasmid were utilized to induce overexpression of miR-184 and DLX1 in Du145 cells, respectively. Cell Counting Kit-8, wound healing and Transwell assays were performed to examine the effects of miR-184 on the aggressiveness of PC cells. Dual-luciferase reporter gene assay was used to investigate the association between miR-184 and DLX1, and reverse transcription-quantitative PCR and western blot analyses were utilized to determine the mRNA and protein levels. miR-184 expression was found to be downregulated whereas DLX1 was upregulated in PC tissues compared with normal prostate tissues. Cell propagation, migration and invasion were all inhibited by miR-184 upregulation in Du145 cells. Dual luciferase reporter assay confirmed the association between miR-184 and DLX1. The inhibitory effect of miR-184 mimic on cell behaviors was reversed by upregulation of DLX1. These findings suggest that miR-184 plays a beneficial role in suppressing the tumorigenesis of PC by directly targeting DLX1, and it may represent a potential therapeutic strategy for PC. D.A. Spandidos 2021-10 2021-08-11 /pmc/articles/PMC8393589/ /pubmed/34504608 http://dx.doi.org/10.3892/etm.2021.10597 Text en Copyright: © Tan et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Tan, Gui-Geng
Xu, Chang
Zhong, Wei-Kang
Wang, Chuan-Yun
miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1
title miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1
title_full miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1
title_fullStr miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1
title_full_unstemmed miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1
title_short miR-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1
title_sort mir-184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing dlx1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393589/
https://www.ncbi.nlm.nih.gov/pubmed/34504608
http://dx.doi.org/10.3892/etm.2021.10597
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