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Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells
Filtration barrier injury induced by high glucose (HG) levels leads to the development of diabetic nephropathy. The endothelial glycocalyx plays a critical role in glomerular barrier function. In the present study, the effects of piperazine ferulate (PF) on HG-induced filtration barrier injury of gl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393711/ https://www.ncbi.nlm.nih.gov/pubmed/34504620 http://dx.doi.org/10.3892/etm.2021.10607 |
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author | Yang, Yong-Yu Chen, Zhuo Yang, Xi-Ding Deng, Rong-Rong Shi, Ling-Xing Yao, Liang-Yuan Xiang, Da-Xiong |
author_facet | Yang, Yong-Yu Chen, Zhuo Yang, Xi-Ding Deng, Rong-Rong Shi, Ling-Xing Yao, Liang-Yuan Xiang, Da-Xiong |
author_sort | Yang, Yong-Yu |
collection | PubMed |
description | Filtration barrier injury induced by high glucose (HG) levels leads to the development of diabetic nephropathy. The endothelial glycocalyx plays a critical role in glomerular barrier function. In the present study, the effects of piperazine ferulate (PF) on HG-induced filtration barrier injury of glomerular endothelial cells (GEnCs) were investigated and the underlying mechanism was assessed. Immunofluorescence was used to observe the distribution of the glycocalyx as well as the expression levels of syndecan-1 and Zonula occludens-1 (ZO-1). Endothelial permeability assays were performed to assess the effects of PF on the integrity of the filtration barrier. Protein and mRNA expression levels were measured by western blotting and reverse transcription-quantitative PCR analyses, respectively. In vitro experiments revealed that adenosine monophosphate-activated protein kinase (AMPK) mediated HG-induced glycocalyx degradation and endothelial barrier injury. PF inhibited the HG-induced endothelial barrier injury and restored the expression levels of heparanase-1 (Hpa-1), ZO-1 and occludin-1 by AMPK. In vivo assays demonstrated that PF reduced the expression levels of Hpa-1, increased the expression levels of ZO-1 and attenuated glycocalyx degradation in the glomerulus. These data suggested that PF attenuated HG-induced filtration barrier injury of GEnC by regulating AMPK expression. |
format | Online Article Text |
id | pubmed-8393711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-83937112021-09-08 Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells Yang, Yong-Yu Chen, Zhuo Yang, Xi-Ding Deng, Rong-Rong Shi, Ling-Xing Yao, Liang-Yuan Xiang, Da-Xiong Exp Ther Med Articles Filtration barrier injury induced by high glucose (HG) levels leads to the development of diabetic nephropathy. The endothelial glycocalyx plays a critical role in glomerular barrier function. In the present study, the effects of piperazine ferulate (PF) on HG-induced filtration barrier injury of glomerular endothelial cells (GEnCs) were investigated and the underlying mechanism was assessed. Immunofluorescence was used to observe the distribution of the glycocalyx as well as the expression levels of syndecan-1 and Zonula occludens-1 (ZO-1). Endothelial permeability assays were performed to assess the effects of PF on the integrity of the filtration barrier. Protein and mRNA expression levels were measured by western blotting and reverse transcription-quantitative PCR analyses, respectively. In vitro experiments revealed that adenosine monophosphate-activated protein kinase (AMPK) mediated HG-induced glycocalyx degradation and endothelial barrier injury. PF inhibited the HG-induced endothelial barrier injury and restored the expression levels of heparanase-1 (Hpa-1), ZO-1 and occludin-1 by AMPK. In vivo assays demonstrated that PF reduced the expression levels of Hpa-1, increased the expression levels of ZO-1 and attenuated glycocalyx degradation in the glomerulus. These data suggested that PF attenuated HG-induced filtration barrier injury of GEnC by regulating AMPK expression. D.A. Spandidos 2021-10 2021-08-13 /pmc/articles/PMC8393711/ /pubmed/34504620 http://dx.doi.org/10.3892/etm.2021.10607 Text en Copyright: © Yang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yang, Yong-Yu Chen, Zhuo Yang, Xi-Ding Deng, Rong-Rong Shi, Ling-Xing Yao, Liang-Yuan Xiang, Da-Xiong Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells |
title | Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells |
title_full | Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells |
title_fullStr | Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells |
title_full_unstemmed | Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells |
title_short | Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells |
title_sort | piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393711/ https://www.ncbi.nlm.nih.gov/pubmed/34504620 http://dx.doi.org/10.3892/etm.2021.10607 |
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