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Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3

Cardiac fibrosis is a core process in the development of heart failure. However, the underlying mechanism of cardiac fibrosis remains unclear. Recently, a study found that in an isoproterenol (ISO)-induced cardiac fibrosis animal model, there is high expression of latent-transforming growth factor β...

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Autores principales: Shi, Chunlai, Li, Xuan, Hong, Fei, Wang, Xueyan, Jiang, Tingting, Sun, Bingbing, Li, Shuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393931/
https://www.ncbi.nlm.nih.gov/pubmed/34504591
http://dx.doi.org/10.3892/etm.2021.10580
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author Shi, Chunlai
Li, Xuan
Hong, Fei
Wang, Xueyan
Jiang, Tingting
Sun, Bingbing
Li, Shuang
author_facet Shi, Chunlai
Li, Xuan
Hong, Fei
Wang, Xueyan
Jiang, Tingting
Sun, Bingbing
Li, Shuang
author_sort Shi, Chunlai
collection PubMed
description Cardiac fibrosis is a core process in the development of heart failure. However, the underlying mechanism of cardiac fibrosis remains unclear. Recently, a study found that in an isoproterenol (ISO)-induced cardiac fibrosis animal model, there is high expression of latent-transforming growth factor β-binding protein 2 (LTBP2) in cardiac fibroblasts. Whether LTBP2 serves a role in cardiac fibrosis is currently unknown. In the present study, mouse cardiac fibroblasts (MCFs) were treated with 100 µM/l ISO for 24, 48, or 72 h, and small interfering RNAs (siRNAs) were used to knockdown LTBP2. Reverse transcription-quantitative PCR and western blotting were used to determine gene and protein expression levels, respectively. Caspase-3 serves a key role in cell apoptosis and is related to cardiac fibrosis-induced heart failure. Caspase-3 activity was therefore determined using a caspase-3 assay kit, CCK8 was used to determine the rate of cell proliferation and apoptosis rates were quantified using a cell death detection ELISA kit. The present study demonstrated that cell apoptosis and LTBP2 expression increased in MCFs treated with 100 µM/l ISO in a time-dependent manner. Expression and activity of caspase-3 also increased in MCFs treated with 100 µM/l ISO for 48 h compared with the control group. In addition, ISO stimulation-induced MCF apoptosis, along with the increased expression of caspase-3 were partly abolished when LTBP2 was knocked down. In conclusion, LTBP2 expression increased in ISO-treated MCFs and accelerated mouse cardiac fibroblast apoptosis by enhancing the expression and activity of caspase-3. LTBP2 may therefore be a potential therapeutic target for treating patients with cardiac fibrosis.
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spelling pubmed-83939312021-09-08 Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3 Shi, Chunlai Li, Xuan Hong, Fei Wang, Xueyan Jiang, Tingting Sun, Bingbing Li, Shuang Exp Ther Med Articles Cardiac fibrosis is a core process in the development of heart failure. However, the underlying mechanism of cardiac fibrosis remains unclear. Recently, a study found that in an isoproterenol (ISO)-induced cardiac fibrosis animal model, there is high expression of latent-transforming growth factor β-binding protein 2 (LTBP2) in cardiac fibroblasts. Whether LTBP2 serves a role in cardiac fibrosis is currently unknown. In the present study, mouse cardiac fibroblasts (MCFs) were treated with 100 µM/l ISO for 24, 48, or 72 h, and small interfering RNAs (siRNAs) were used to knockdown LTBP2. Reverse transcription-quantitative PCR and western blotting were used to determine gene and protein expression levels, respectively. Caspase-3 serves a key role in cell apoptosis and is related to cardiac fibrosis-induced heart failure. Caspase-3 activity was therefore determined using a caspase-3 assay kit, CCK8 was used to determine the rate of cell proliferation and apoptosis rates were quantified using a cell death detection ELISA kit. The present study demonstrated that cell apoptosis and LTBP2 expression increased in MCFs treated with 100 µM/l ISO in a time-dependent manner. Expression and activity of caspase-3 also increased in MCFs treated with 100 µM/l ISO for 48 h compared with the control group. In addition, ISO stimulation-induced MCF apoptosis, along with the increased expression of caspase-3 were partly abolished when LTBP2 was knocked down. In conclusion, LTBP2 expression increased in ISO-treated MCFs and accelerated mouse cardiac fibroblast apoptosis by enhancing the expression and activity of caspase-3. LTBP2 may therefore be a potential therapeutic target for treating patients with cardiac fibrosis. D.A. Spandidos 2021-10 2021-08-09 /pmc/articles/PMC8393931/ /pubmed/34504591 http://dx.doi.org/10.3892/etm.2021.10580 Text en Copyright: © Shi et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Shi, Chunlai
Li, Xuan
Hong, Fei
Wang, Xueyan
Jiang, Tingting
Sun, Bingbing
Li, Shuang
Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3
title Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3
title_full Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3
title_fullStr Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3
title_full_unstemmed Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3
title_short Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3
title_sort latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393931/
https://www.ncbi.nlm.nih.gov/pubmed/34504591
http://dx.doi.org/10.3892/etm.2021.10580
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