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Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer

Identification of markers predicting disease outcome is a major clinical issue for non-muscle invasive bladder cancer (NMIBC). The present study aimed to determine the role of the mitochondrial proteins Mitofusin-2 (Mfn2) and caseinolytic protease P (ClpP) in predicting the outcome of NMIBC. The stu...

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Autores principales: Cormio, Antonella, Busetto, Gian Maria, Musicco, Clara, Sanguedolce, Francesca, Calò, Beppe, Chirico, Marco, Falagario, Ugo Giovanni, Carrieri, Giuseppe, Piccoli, Claudia, Cormio, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394056/
https://www.ncbi.nlm.nih.gov/pubmed/34441434
http://dx.doi.org/10.3390/diagnostics11081500
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author Cormio, Antonella
Busetto, Gian Maria
Musicco, Clara
Sanguedolce, Francesca
Calò, Beppe
Chirico, Marco
Falagario, Ugo Giovanni
Carrieri, Giuseppe
Piccoli, Claudia
Cormio, Luigi
author_facet Cormio, Antonella
Busetto, Gian Maria
Musicco, Clara
Sanguedolce, Francesca
Calò, Beppe
Chirico, Marco
Falagario, Ugo Giovanni
Carrieri, Giuseppe
Piccoli, Claudia
Cormio, Luigi
author_sort Cormio, Antonella
collection PubMed
description Identification of markers predicting disease outcome is a major clinical issue for non-muscle invasive bladder cancer (NMIBC). The present study aimed to determine the role of the mitochondrial proteins Mitofusin-2 (Mfn2) and caseinolytic protease P (ClpP) in predicting the outcome of NMIBC. The study population consisted of patients scheduled for transurethral resection of bladder tumor upon the clinical diagnosis of bladder cancer (BC). Samples of the main bladder tumor and healthy-looking bladder wall from patients classified as NMIBC were tested for Mfn2 and ClpP. The expression levels of these proteins were correlated to disease recurrence, progression. Mfn2 and ClpP expression levels were significantly higher in lesional than in non-lesional tissue. Low-risk NMIBC had significantly higher Mfn2 expression levels and significantly lower ClpP expression levels than high-risk NMIBC; there were no differences in non-lesional levels of the two proteins. Lesional Mfn2 expression levels were significantly lower in patients who progressed whereas ClpP levels had no impact on any survival outcome. Multivariable analysis adjusting for the EORTC scores showed that Mfn2 downregulation was significantly associated with disease progression. In conclusion, Mfn2 and ClpP proteins were found to be overexpressed in BC as compared to non-lesional bladder tissue and Mfn2 expression predicted disease progression.
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spelling pubmed-83940562021-08-28 Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer Cormio, Antonella Busetto, Gian Maria Musicco, Clara Sanguedolce, Francesca Calò, Beppe Chirico, Marco Falagario, Ugo Giovanni Carrieri, Giuseppe Piccoli, Claudia Cormio, Luigi Diagnostics (Basel) Article Identification of markers predicting disease outcome is a major clinical issue for non-muscle invasive bladder cancer (NMIBC). The present study aimed to determine the role of the mitochondrial proteins Mitofusin-2 (Mfn2) and caseinolytic protease P (ClpP) in predicting the outcome of NMIBC. The study population consisted of patients scheduled for transurethral resection of bladder tumor upon the clinical diagnosis of bladder cancer (BC). Samples of the main bladder tumor and healthy-looking bladder wall from patients classified as NMIBC were tested for Mfn2 and ClpP. The expression levels of these proteins were correlated to disease recurrence, progression. Mfn2 and ClpP expression levels were significantly higher in lesional than in non-lesional tissue. Low-risk NMIBC had significantly higher Mfn2 expression levels and significantly lower ClpP expression levels than high-risk NMIBC; there were no differences in non-lesional levels of the two proteins. Lesional Mfn2 expression levels were significantly lower in patients who progressed whereas ClpP levels had no impact on any survival outcome. Multivariable analysis adjusting for the EORTC scores showed that Mfn2 downregulation was significantly associated with disease progression. In conclusion, Mfn2 and ClpP proteins were found to be overexpressed in BC as compared to non-lesional bladder tissue and Mfn2 expression predicted disease progression. MDPI 2021-08-20 /pmc/articles/PMC8394056/ /pubmed/34441434 http://dx.doi.org/10.3390/diagnostics11081500 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cormio, Antonella
Busetto, Gian Maria
Musicco, Clara
Sanguedolce, Francesca
Calò, Beppe
Chirico, Marco
Falagario, Ugo Giovanni
Carrieri, Giuseppe
Piccoli, Claudia
Cormio, Luigi
Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
title Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
title_full Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
title_fullStr Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
title_full_unstemmed Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
title_short Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
title_sort mitofusin-2 down-regulation predicts progression of non-muscle invasive bladder cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394056/
https://www.ncbi.nlm.nih.gov/pubmed/34441434
http://dx.doi.org/10.3390/diagnostics11081500
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