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Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis

In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8...

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Detalles Bibliográficos
Autores principales: Wang, Chunxue, Yi, Pengfei, Li, Jiang, Dong, Haibing, Chen, Changming, Zhang, Daming, Shen, Haiqing, Fu, Bendong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394289/
https://www.ncbi.nlm.nih.gov/pubmed/34436066
http://dx.doi.org/10.3390/bios11080264
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author Wang, Chunxue
Yi, Pengfei
Li, Jiang
Dong, Haibing
Chen, Changming
Zhang, Daming
Shen, Haiqing
Fu, Bendong
author_facet Wang, Chunxue
Yi, Pengfei
Li, Jiang
Dong, Haibing
Chen, Changming
Zhang, Daming
Shen, Haiqing
Fu, Bendong
author_sort Wang, Chunxue
collection PubMed
description In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8 (FSU-8) as the sensing core layer and polymethyl methacrylate (PMMA) as the sensing window cladding were synthesized. The effective drug concentration range (5–10 µg/mL) of ginkgolide A for inhibition of PMVEC apoptosis was analyzed and obtained by pharmacological studies. The structure of the device was optimized to be designed and fabricated by direct UV writing technology. The properties of the biosensor were simulated with various refractive indices of different drug concentrations. The actual sensitivity of the biosensor was measured as 1606.2 nm/RIU. The resolution and detection limit were characterized as 0.05 nm and 3 × 10(−5) RIU, respectively. The technique is suitable for safe and accurate detection of effective organic drug dosages of Chinese herbal ingredients.
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spelling pubmed-83942892021-08-28 Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis Wang, Chunxue Yi, Pengfei Li, Jiang Dong, Haibing Chen, Changming Zhang, Daming Shen, Haiqing Fu, Bendong Biosensors (Basel) Communication In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8 (FSU-8) as the sensing core layer and polymethyl methacrylate (PMMA) as the sensing window cladding were synthesized. The effective drug concentration range (5–10 µg/mL) of ginkgolide A for inhibition of PMVEC apoptosis was analyzed and obtained by pharmacological studies. The structure of the device was optimized to be designed and fabricated by direct UV writing technology. The properties of the biosensor were simulated with various refractive indices of different drug concentrations. The actual sensitivity of the biosensor was measured as 1606.2 nm/RIU. The resolution and detection limit were characterized as 0.05 nm and 3 × 10(−5) RIU, respectively. The technique is suitable for safe and accurate detection of effective organic drug dosages of Chinese herbal ingredients. MDPI 2021-08-06 /pmc/articles/PMC8394289/ /pubmed/34436066 http://dx.doi.org/10.3390/bios11080264 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wang, Chunxue
Yi, Pengfei
Li, Jiang
Dong, Haibing
Chen, Changming
Zhang, Daming
Shen, Haiqing
Fu, Bendong
Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
title Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
title_full Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
title_fullStr Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
title_full_unstemmed Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
title_short Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
title_sort polymer optical waveguide grating-based biosensor to detect effective drug concentrations of ginkgolide a for inhibition of pmvec apoptosis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394289/
https://www.ncbi.nlm.nih.gov/pubmed/34436066
http://dx.doi.org/10.3390/bios11080264
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