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Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine

Cultured fibroblast progenitor cells (FPC) have been studied in Swiss translational regenerative medicine for over two decades, wherein clinical experience was gathered for safely managing burns and refractory cutaneous ulcers. Inherent FPC advantages include high robustness, optimal adaptability to...

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Autores principales: Laurent, Alexis, Scaletta, Corinne, Abdel-Sayed, Philippe, Michetti, Murielle, Flahaut, Marjorie, Simon, Jeanne-Pascale, Roessingh, Anthony de Buys, Raffoul, Wassim, Hirt-Burri, Nathalie, Applegate, Lee Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394413/
https://www.ncbi.nlm.nih.gov/pubmed/34440276
http://dx.doi.org/10.3390/biomedicines9081072
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author Laurent, Alexis
Scaletta, Corinne
Abdel-Sayed, Philippe
Michetti, Murielle
Flahaut, Marjorie
Simon, Jeanne-Pascale
Roessingh, Anthony de Buys
Raffoul, Wassim
Hirt-Burri, Nathalie
Applegate, Lee Ann
author_facet Laurent, Alexis
Scaletta, Corinne
Abdel-Sayed, Philippe
Michetti, Murielle
Flahaut, Marjorie
Simon, Jeanne-Pascale
Roessingh, Anthony de Buys
Raffoul, Wassim
Hirt-Burri, Nathalie
Applegate, Lee Ann
author_sort Laurent, Alexis
collection PubMed
description Cultured fibroblast progenitor cells (FPC) have been studied in Swiss translational regenerative medicine for over two decades, wherein clinical experience was gathered for safely managing burns and refractory cutaneous ulcers. Inherent FPC advantages include high robustness, optimal adaptability to industrial manufacture, and potential for effective repair stimulation of wounded tissues. Major technical bottlenecks in cell therapy development comprise sustainability, stability, and logistics of biological material sources. Herein, we report stringently optimized and up-scaled processing (i.e., cell biobanking and stabilization by lyophilization) of dermal FPCs, with the objective of addressing potential cell source sustainability and stability issues with regard to active substance manufacturing in cutaneous regenerative medicine. Firstly, multi-tiered FPC banking was optimized in terms of overall quality and efficiency by benchmarking key reagents (e.g., medium supplement source, dissociation reagent), consumables (e.g., culture vessels), and technical specifications. Therein, fetal bovine serum batch identity and culture vessel surface were confirmed, among other parameters, to largely impact harvest cell yields. Secondly, FPC stabilization by lyophilization was undertaken and shown to maintain critical functions for devitalized cells in vitro, potentially enabling high logistical gains. Overall, this study provides the technical basis for the elaboration of next-generation off-the-shelf topical regenerative medicine therapeutic products for wound healing and post-burn care.
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spelling pubmed-83944132021-08-28 Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine Laurent, Alexis Scaletta, Corinne Abdel-Sayed, Philippe Michetti, Murielle Flahaut, Marjorie Simon, Jeanne-Pascale Roessingh, Anthony de Buys Raffoul, Wassim Hirt-Burri, Nathalie Applegate, Lee Ann Biomedicines Article Cultured fibroblast progenitor cells (FPC) have been studied in Swiss translational regenerative medicine for over two decades, wherein clinical experience was gathered for safely managing burns and refractory cutaneous ulcers. Inherent FPC advantages include high robustness, optimal adaptability to industrial manufacture, and potential for effective repair stimulation of wounded tissues. Major technical bottlenecks in cell therapy development comprise sustainability, stability, and logistics of biological material sources. Herein, we report stringently optimized and up-scaled processing (i.e., cell biobanking and stabilization by lyophilization) of dermal FPCs, with the objective of addressing potential cell source sustainability and stability issues with regard to active substance manufacturing in cutaneous regenerative medicine. Firstly, multi-tiered FPC banking was optimized in terms of overall quality and efficiency by benchmarking key reagents (e.g., medium supplement source, dissociation reagent), consumables (e.g., culture vessels), and technical specifications. Therein, fetal bovine serum batch identity and culture vessel surface were confirmed, among other parameters, to largely impact harvest cell yields. Secondly, FPC stabilization by lyophilization was undertaken and shown to maintain critical functions for devitalized cells in vitro, potentially enabling high logistical gains. Overall, this study provides the technical basis for the elaboration of next-generation off-the-shelf topical regenerative medicine therapeutic products for wound healing and post-burn care. MDPI 2021-08-23 /pmc/articles/PMC8394413/ /pubmed/34440276 http://dx.doi.org/10.3390/biomedicines9081072 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Laurent, Alexis
Scaletta, Corinne
Abdel-Sayed, Philippe
Michetti, Murielle
Flahaut, Marjorie
Simon, Jeanne-Pascale
Roessingh, Anthony de Buys
Raffoul, Wassim
Hirt-Burri, Nathalie
Applegate, Lee Ann
Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine
title Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine
title_full Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine
title_fullStr Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine
title_full_unstemmed Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine
title_short Optimized Manufacture of Lyophilized Dermal Fibroblasts for Next-Generation Off-the-Shelf Progenitor Biological Bandages in Topical Post-Burn Regenerative Medicine
title_sort optimized manufacture of lyophilized dermal fibroblasts for next-generation off-the-shelf progenitor biological bandages in topical post-burn regenerative medicine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394413/
https://www.ncbi.nlm.nih.gov/pubmed/34440276
http://dx.doi.org/10.3390/biomedicines9081072
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