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Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population

Heat shock protein 70 (Hsp70) engages Toll-like receptors (TLR) 2 and 4 when found in the extracellular compartment and contributes to inflammation in chronic obstructive pulmonary disease (COPD). Since there is growing evidence for the genetic risk factors for COPD, the gene expression of HSP70, TL...

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Autores principales: Hlapčić, Iva, Grdić Rajković, Marija, Čeri, Andrea, Dabelić, Sanja, Popović-Grle, Sanja, Radić Antolic, Margareta, Knežević, Jelena, Försti, Asta, Rumora, Lada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394658/
https://www.ncbi.nlm.nih.gov/pubmed/34441346
http://dx.doi.org/10.3390/diagnostics11081412
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author Hlapčić, Iva
Grdić Rajković, Marija
Čeri, Andrea
Dabelić, Sanja
Popović-Grle, Sanja
Radić Antolic, Margareta
Knežević, Jelena
Försti, Asta
Rumora, Lada
author_facet Hlapčić, Iva
Grdić Rajković, Marija
Čeri, Andrea
Dabelić, Sanja
Popović-Grle, Sanja
Radić Antolic, Margareta
Knežević, Jelena
Försti, Asta
Rumora, Lada
author_sort Hlapčić, Iva
collection PubMed
description Heat shock protein 70 (Hsp70) engages Toll-like receptors (TLR) 2 and 4 when found in the extracellular compartment and contributes to inflammation in chronic obstructive pulmonary disease (COPD). Since there is growing evidence for the genetic risk factors for COPD, the gene expression of HSP70, TLR2 and TLR4 was determined, as well as the association between HSP70, TLR2 and TLR4 single nucleotide polymorphisms, (SNPs) and COPD. The gene expression was assessed in peripheral blood cells of 137 COPD patients and 95 controls by a quantitative polymerase chain reaction (qPCR), while a total of nine SNPs were genotyped by TaqMan allelic discrimination real-time PCR. HSP70 and TLR2 gene expression was increased in COPD patients compared to the controls, regardless of the disease severity and smoking status of participants. The rs6457452 SNP of HSP70 was associated with COPD, indicating the protective role of the T allele (OR = 0.46, 95% CI = 0.24–0.89, p = 0.022). Furthermore, COPD C/T heterozygotes showed a decreased HSP70 mRNA level compared to COPD C/C homozygotes. In conclusion, HSP70 and TLR2 may have a role in the pathogenesis of COPD, and the HSP70 rs6457452 variant might influence the genetic susceptibility to COPD in the Croatian population.
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spelling pubmed-83946582021-08-28 Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population Hlapčić, Iva Grdić Rajković, Marija Čeri, Andrea Dabelić, Sanja Popović-Grle, Sanja Radić Antolic, Margareta Knežević, Jelena Försti, Asta Rumora, Lada Diagnostics (Basel) Article Heat shock protein 70 (Hsp70) engages Toll-like receptors (TLR) 2 and 4 when found in the extracellular compartment and contributes to inflammation in chronic obstructive pulmonary disease (COPD). Since there is growing evidence for the genetic risk factors for COPD, the gene expression of HSP70, TLR2 and TLR4 was determined, as well as the association between HSP70, TLR2 and TLR4 single nucleotide polymorphisms, (SNPs) and COPD. The gene expression was assessed in peripheral blood cells of 137 COPD patients and 95 controls by a quantitative polymerase chain reaction (qPCR), while a total of nine SNPs were genotyped by TaqMan allelic discrimination real-time PCR. HSP70 and TLR2 gene expression was increased in COPD patients compared to the controls, regardless of the disease severity and smoking status of participants. The rs6457452 SNP of HSP70 was associated with COPD, indicating the protective role of the T allele (OR = 0.46, 95% CI = 0.24–0.89, p = 0.022). Furthermore, COPD C/T heterozygotes showed a decreased HSP70 mRNA level compared to COPD C/C homozygotes. In conclusion, HSP70 and TLR2 may have a role in the pathogenesis of COPD, and the HSP70 rs6457452 variant might influence the genetic susceptibility to COPD in the Croatian population. MDPI 2021-08-04 /pmc/articles/PMC8394658/ /pubmed/34441346 http://dx.doi.org/10.3390/diagnostics11081412 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hlapčić, Iva
Grdić Rajković, Marija
Čeri, Andrea
Dabelić, Sanja
Popović-Grle, Sanja
Radić Antolic, Margareta
Knežević, Jelena
Försti, Asta
Rumora, Lada
Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population
title Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population
title_full Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population
title_fullStr Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population
title_full_unstemmed Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population
title_short Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population
title_sort increased hsp70 and tlr2 gene expression and association of hsp70 rs6457452 single nucleotide polymorphism with the risk of chronic obstructive pulmonary disease in the croatian population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394658/
https://www.ncbi.nlm.nih.gov/pubmed/34441346
http://dx.doi.org/10.3390/diagnostics11081412
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