Cargando…

Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants

Leaf senescence, the last stage of leaf development, is a well-regulated and complex process for investigation. For simplification, dark-induced leaf senescence has frequently been used to mimic the natural senescence of leaves because many typical senescence symptoms, such as chlorophyll (Chl) and...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Xueyun, Zeng, Chu, Su, Jinling, Khan, Imran, Zada, Ahmad, Jia, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394709/
https://www.ncbi.nlm.nih.gov/pubmed/34439809
http://dx.doi.org/10.3390/biom11081143
_version_ 1783744009698541568
author Hu, Xueyun
Zeng, Chu
Su, Jinling
Khan, Imran
Zada, Ahmad
Jia, Ting
author_facet Hu, Xueyun
Zeng, Chu
Su, Jinling
Khan, Imran
Zada, Ahmad
Jia, Ting
author_sort Hu, Xueyun
collection PubMed
description Leaf senescence, the last stage of leaf development, is a well-regulated and complex process for investigation. For simplification, dark-induced leaf senescence has frequently been used to mimic the natural senescence of leaves because many typical senescence symptoms, such as chlorophyll (Chl) and protein degradation, also occur under darkness. In this study, we compared the phenotypes of leaf senescence that occurred when detached leaves or intact plants were incubated in darkness to induce senescence. We found that the symptoms of non-programmed cell death (non-PCD) with remaining green coloration occurred more heavily in the senescent leaves of whole plants than in the detached leaves. The pheophorbide a (Pheide a) content was also shown to be much higher in senescent leaves when whole plants were incubated in darkness by analyses of leaf Chl and its metabolic intermediates. In addition, more serious non-PCD occurred and more Pheide a accumulated in senescent leaves during dark incubation if the soil used for plant growth contained more water. Under similar conditions, the non-PCD phenotype was alleviated and the accumulation of Pheide a was reduced by overexpressing 7-hydroxymethyl Chl a (HMChl a) reductase (HCAR). Taken together, we conclude that a high soil water content induced non-PCD by decreasing HCAR activity when whole plants were incubated in darkness to induce senescence; thus, the investigation of the fundamental aspects of biochemistry and the regulation of leaf senescence are affected by using dark-induced leaf senescence.
format Online
Article
Text
id pubmed-8394709
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83947092021-08-28 Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants Hu, Xueyun Zeng, Chu Su, Jinling Khan, Imran Zada, Ahmad Jia, Ting Biomolecules Article Leaf senescence, the last stage of leaf development, is a well-regulated and complex process for investigation. For simplification, dark-induced leaf senescence has frequently been used to mimic the natural senescence of leaves because many typical senescence symptoms, such as chlorophyll (Chl) and protein degradation, also occur under darkness. In this study, we compared the phenotypes of leaf senescence that occurred when detached leaves or intact plants were incubated in darkness to induce senescence. We found that the symptoms of non-programmed cell death (non-PCD) with remaining green coloration occurred more heavily in the senescent leaves of whole plants than in the detached leaves. The pheophorbide a (Pheide a) content was also shown to be much higher in senescent leaves when whole plants were incubated in darkness by analyses of leaf Chl and its metabolic intermediates. In addition, more serious non-PCD occurred and more Pheide a accumulated in senescent leaves during dark incubation if the soil used for plant growth contained more water. Under similar conditions, the non-PCD phenotype was alleviated and the accumulation of Pheide a was reduced by overexpressing 7-hydroxymethyl Chl a (HMChl a) reductase (HCAR). Taken together, we conclude that a high soil water content induced non-PCD by decreasing HCAR activity when whole plants were incubated in darkness to induce senescence; thus, the investigation of the fundamental aspects of biochemistry and the regulation of leaf senescence are affected by using dark-induced leaf senescence. MDPI 2021-08-03 /pmc/articles/PMC8394709/ /pubmed/34439809 http://dx.doi.org/10.3390/biom11081143 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Xueyun
Zeng, Chu
Su, Jinling
Khan, Imran
Zada, Ahmad
Jia, Ting
Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants
title Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants
title_full Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants
title_fullStr Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants
title_full_unstemmed Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants
title_short Overexpressing 7-Hydroxymethyl Chlorophyll a Reductase Alleviates Non-Programmed Cell Death during Dark-Induced Senescence in Intact Arabidopsis Plants
title_sort overexpressing 7-hydroxymethyl chlorophyll a reductase alleviates non-programmed cell death during dark-induced senescence in intact arabidopsis plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394709/
https://www.ncbi.nlm.nih.gov/pubmed/34439809
http://dx.doi.org/10.3390/biom11081143
work_keys_str_mv AT huxueyun overexpressing7hydroxymethylchlorophyllareductasealleviatesnonprogrammedcelldeathduringdarkinducedsenescenceinintactarabidopsisplants
AT zengchu overexpressing7hydroxymethylchlorophyllareductasealleviatesnonprogrammedcelldeathduringdarkinducedsenescenceinintactarabidopsisplants
AT sujinling overexpressing7hydroxymethylchlorophyllareductasealleviatesnonprogrammedcelldeathduringdarkinducedsenescenceinintactarabidopsisplants
AT khanimran overexpressing7hydroxymethylchlorophyllareductasealleviatesnonprogrammedcelldeathduringdarkinducedsenescenceinintactarabidopsisplants
AT zadaahmad overexpressing7hydroxymethylchlorophyllareductasealleviatesnonprogrammedcelldeathduringdarkinducedsenescenceinintactarabidopsisplants
AT jiating overexpressing7hydroxymethylchlorophyllareductasealleviatesnonprogrammedcelldeathduringdarkinducedsenescenceinintactarabidopsisplants