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Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations

How dentists cure a resin-based material has deleterious effects on the material’s properties and its interaction with surrounding dental tissues. Biofilm accumulation has been implicated in the pathogenesis of carious lesions around dental restorations, with its composition manifesting expressed dy...

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Autores principales: Maktabi, Haifa, Ibrahim, Maria Salem, Balhaddad, Abdulrahman A., Alkhubaizi, Qoot, Garcia, Isadora Martini, Collares, Fabrício Mezzomo, Strassler, Howard, Fugolin, Ana Paula P., Pfeifer, Carmem S., Melo, Mary Anne S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394739/
https://www.ncbi.nlm.nih.gov/pubmed/34435995
http://dx.doi.org/10.3390/dj9080083
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author Maktabi, Haifa
Ibrahim, Maria Salem
Balhaddad, Abdulrahman A.
Alkhubaizi, Qoot
Garcia, Isadora Martini
Collares, Fabrício Mezzomo
Strassler, Howard
Fugolin, Ana Paula P.
Pfeifer, Carmem S.
Melo, Mary Anne S.
author_facet Maktabi, Haifa
Ibrahim, Maria Salem
Balhaddad, Abdulrahman A.
Alkhubaizi, Qoot
Garcia, Isadora Martini
Collares, Fabrício Mezzomo
Strassler, Howard
Fugolin, Ana Paula P.
Pfeifer, Carmem S.
Melo, Mary Anne S.
author_sort Maktabi, Haifa
collection PubMed
description How dentists cure a resin-based material has deleterious effects on the material’s properties and its interaction with surrounding dental tissues. Biofilm accumulation has been implicated in the pathogenesis of carious lesions around dental restorations, with its composition manifesting expressed dysbiosis in patients suffering from dental caries. To evaluate the influence of varying radiant exposure on the degree of conversion (DC%), Streptococcus mutans biofilm growth, and surface roughness of bulk-fill composites under different light-curing conditions. Two light-curing units (LCU) at 600 and 1000 mW/cm(2) were used to simulate curing conditions with different angulations (∢20° and ∢35°) or 2 mm-distance displacements of the LCU tip. The radiant exposure (RE) was assessed, and the composites were analyzed for DC%. Biofilm formation was induced over the bulk-fill composites and analyzed via colony-forming units counting and scanning electron microscopy (SEM). The surface roughness was analyzed via a profilometer and SEM after biofilm formation. Curing conditions with different angulation or displacement decreased RE compared to the “optimal condition”. The moderately (∢35°) angulated LCU tip and low (600 mW/cm(2)) radiant emittance significantly reduced the DC% (p < 0.05). The difference in DC% between the top and bottom of the composites ranged from 8 to 11% for 600 mW/cm(2) and 10 to 20% for 1000 mW/cm(2). Greater S. mutans biofilm and surface changes were found in composites with non-optimal RE delivery (e.g., tip displacement and angulation) (p < 0.05). Inadequate polymerization of bulk-fill composites was associated with more biofilm accumulation and surface topography changes. Overall, non-optimally performed curing procedures reduced the amount of delivered RE, which led to low DC%, more biofilm formation, and higher surface roughness. The improper light-curing of bulk-fill composites compromises their physicochemical and biological properties, which could lead to inferior clinical performance and reduced restorative treatments’ longevity.
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spelling pubmed-83947392021-08-28 Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations Maktabi, Haifa Ibrahim, Maria Salem Balhaddad, Abdulrahman A. Alkhubaizi, Qoot Garcia, Isadora Martini Collares, Fabrício Mezzomo Strassler, Howard Fugolin, Ana Paula P. Pfeifer, Carmem S. Melo, Mary Anne S. Dent J (Basel) Article How dentists cure a resin-based material has deleterious effects on the material’s properties and its interaction with surrounding dental tissues. Biofilm accumulation has been implicated in the pathogenesis of carious lesions around dental restorations, with its composition manifesting expressed dysbiosis in patients suffering from dental caries. To evaluate the influence of varying radiant exposure on the degree of conversion (DC%), Streptococcus mutans biofilm growth, and surface roughness of bulk-fill composites under different light-curing conditions. Two light-curing units (LCU) at 600 and 1000 mW/cm(2) were used to simulate curing conditions with different angulations (∢20° and ∢35°) or 2 mm-distance displacements of the LCU tip. The radiant exposure (RE) was assessed, and the composites were analyzed for DC%. Biofilm formation was induced over the bulk-fill composites and analyzed via colony-forming units counting and scanning electron microscopy (SEM). The surface roughness was analyzed via a profilometer and SEM after biofilm formation. Curing conditions with different angulation or displacement decreased RE compared to the “optimal condition”. The moderately (∢35°) angulated LCU tip and low (600 mW/cm(2)) radiant emittance significantly reduced the DC% (p < 0.05). The difference in DC% between the top and bottom of the composites ranged from 8 to 11% for 600 mW/cm(2) and 10 to 20% for 1000 mW/cm(2). Greater S. mutans biofilm and surface changes were found in composites with non-optimal RE delivery (e.g., tip displacement and angulation) (p < 0.05). Inadequate polymerization of bulk-fill composites was associated with more biofilm accumulation and surface topography changes. Overall, non-optimally performed curing procedures reduced the amount of delivered RE, which led to low DC%, more biofilm formation, and higher surface roughness. The improper light-curing of bulk-fill composites compromises their physicochemical and biological properties, which could lead to inferior clinical performance and reduced restorative treatments’ longevity. MDPI 2021-07-30 /pmc/articles/PMC8394739/ /pubmed/34435995 http://dx.doi.org/10.3390/dj9080083 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maktabi, Haifa
Ibrahim, Maria Salem
Balhaddad, Abdulrahman A.
Alkhubaizi, Qoot
Garcia, Isadora Martini
Collares, Fabrício Mezzomo
Strassler, Howard
Fugolin, Ana Paula P.
Pfeifer, Carmem S.
Melo, Mary Anne S.
Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations
title Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations
title_full Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations
title_fullStr Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations
title_full_unstemmed Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations
title_short Improper Light Curing of Bulkfill Composite Drives Surface Changes and Increases S. mutans Biofilm Growth as a Pathway for Higher Risk of Recurrent Caries around Restorations
title_sort improper light curing of bulkfill composite drives surface changes and increases s. mutans biofilm growth as a pathway for higher risk of recurrent caries around restorations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394739/
https://www.ncbi.nlm.nih.gov/pubmed/34435995
http://dx.doi.org/10.3390/dj9080083
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