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Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes
Trajectories of endosomes inside living eukaryotic cells are highly heterogeneous in space and time and diffuse anomalously due to a combination of viscoelasticity, caging, aggregation and active transport. Some of the trajectories display switching between persistent and anti-persistent motion, whi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394768/ https://www.ncbi.nlm.nih.gov/pubmed/34441098 http://dx.doi.org/10.3390/e23080958 |
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author | Korabel, Nickolay Han, Daniel Taloni, Alessandro Pagnini, Gianni Fedotov, Sergei Allan, Viki Waigh, Thomas Andrew |
author_facet | Korabel, Nickolay Han, Daniel Taloni, Alessandro Pagnini, Gianni Fedotov, Sergei Allan, Viki Waigh, Thomas Andrew |
author_sort | Korabel, Nickolay |
collection | PubMed |
description | Trajectories of endosomes inside living eukaryotic cells are highly heterogeneous in space and time and diffuse anomalously due to a combination of viscoelasticity, caging, aggregation and active transport. Some of the trajectories display switching between persistent and anti-persistent motion, while others jiggle around in one position for the whole measurement time. By splitting the ensemble of endosome trajectories into slow moving subdiffusive and fast moving superdiffusive endosomes, we analyzed them separately. The mean squared displacements and velocity auto-correlation functions confirm the effectiveness of the splitting methods. Applying the local analysis, we show that both ensembles are characterized by a spectrum of local anomalous exponents and local generalized diffusion coefficients. Slow and fast endosomes have exponential distributions of local anomalous exponents and power law distributions of generalized diffusion coefficients. This suggests that heterogeneous fractional Brownian motion is an appropriate model for both fast and slow moving endosomes. This article is part of a Special Issue entitled: “Recent Advances In Single-Particle Tracking: Experiment and Analysis” edited by Janusz Szwabiński and Aleksander Weron. |
format | Online Article Text |
id | pubmed-8394768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83947682021-08-28 Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes Korabel, Nickolay Han, Daniel Taloni, Alessandro Pagnini, Gianni Fedotov, Sergei Allan, Viki Waigh, Thomas Andrew Entropy (Basel) Article Trajectories of endosomes inside living eukaryotic cells are highly heterogeneous in space and time and diffuse anomalously due to a combination of viscoelasticity, caging, aggregation and active transport. Some of the trajectories display switching between persistent and anti-persistent motion, while others jiggle around in one position for the whole measurement time. By splitting the ensemble of endosome trajectories into slow moving subdiffusive and fast moving superdiffusive endosomes, we analyzed them separately. The mean squared displacements and velocity auto-correlation functions confirm the effectiveness of the splitting methods. Applying the local analysis, we show that both ensembles are characterized by a spectrum of local anomalous exponents and local generalized diffusion coefficients. Slow and fast endosomes have exponential distributions of local anomalous exponents and power law distributions of generalized diffusion coefficients. This suggests that heterogeneous fractional Brownian motion is an appropriate model for both fast and slow moving endosomes. This article is part of a Special Issue entitled: “Recent Advances In Single-Particle Tracking: Experiment and Analysis” edited by Janusz Szwabiński and Aleksander Weron. MDPI 2021-07-27 /pmc/articles/PMC8394768/ /pubmed/34441098 http://dx.doi.org/10.3390/e23080958 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Korabel, Nickolay Han, Daniel Taloni, Alessandro Pagnini, Gianni Fedotov, Sergei Allan, Viki Waigh, Thomas Andrew Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes |
title | Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes |
title_full | Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes |
title_fullStr | Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes |
title_full_unstemmed | Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes |
title_short | Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes |
title_sort | local analysis of heterogeneous intracellular transport: slow and fast moving endosomes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394768/ https://www.ncbi.nlm.nih.gov/pubmed/34441098 http://dx.doi.org/10.3390/e23080958 |
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