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Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes

Trajectories of endosomes inside living eukaryotic cells are highly heterogeneous in space and time and diffuse anomalously due to a combination of viscoelasticity, caging, aggregation and active transport. Some of the trajectories display switching between persistent and anti-persistent motion, whi...

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Detalles Bibliográficos
Autores principales: Korabel, Nickolay, Han, Daniel, Taloni, Alessandro, Pagnini, Gianni, Fedotov, Sergei, Allan, Viki, Waigh, Thomas Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394768/
https://www.ncbi.nlm.nih.gov/pubmed/34441098
http://dx.doi.org/10.3390/e23080958
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author Korabel, Nickolay
Han, Daniel
Taloni, Alessandro
Pagnini, Gianni
Fedotov, Sergei
Allan, Viki
Waigh, Thomas Andrew
author_facet Korabel, Nickolay
Han, Daniel
Taloni, Alessandro
Pagnini, Gianni
Fedotov, Sergei
Allan, Viki
Waigh, Thomas Andrew
author_sort Korabel, Nickolay
collection PubMed
description Trajectories of endosomes inside living eukaryotic cells are highly heterogeneous in space and time and diffuse anomalously due to a combination of viscoelasticity, caging, aggregation and active transport. Some of the trajectories display switching between persistent and anti-persistent motion, while others jiggle around in one position for the whole measurement time. By splitting the ensemble of endosome trajectories into slow moving subdiffusive and fast moving superdiffusive endosomes, we analyzed them separately. The mean squared displacements and velocity auto-correlation functions confirm the effectiveness of the splitting methods. Applying the local analysis, we show that both ensembles are characterized by a spectrum of local anomalous exponents and local generalized diffusion coefficients. Slow and fast endosomes have exponential distributions of local anomalous exponents and power law distributions of generalized diffusion coefficients. This suggests that heterogeneous fractional Brownian motion is an appropriate model for both fast and slow moving endosomes. This article is part of a Special Issue entitled: “Recent Advances In Single-Particle Tracking: Experiment and Analysis” edited by Janusz Szwabiński and Aleksander Weron.
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spelling pubmed-83947682021-08-28 Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes Korabel, Nickolay Han, Daniel Taloni, Alessandro Pagnini, Gianni Fedotov, Sergei Allan, Viki Waigh, Thomas Andrew Entropy (Basel) Article Trajectories of endosomes inside living eukaryotic cells are highly heterogeneous in space and time and diffuse anomalously due to a combination of viscoelasticity, caging, aggregation and active transport. Some of the trajectories display switching between persistent and anti-persistent motion, while others jiggle around in one position for the whole measurement time. By splitting the ensemble of endosome trajectories into slow moving subdiffusive and fast moving superdiffusive endosomes, we analyzed them separately. The mean squared displacements and velocity auto-correlation functions confirm the effectiveness of the splitting methods. Applying the local analysis, we show that both ensembles are characterized by a spectrum of local anomalous exponents and local generalized diffusion coefficients. Slow and fast endosomes have exponential distributions of local anomalous exponents and power law distributions of generalized diffusion coefficients. This suggests that heterogeneous fractional Brownian motion is an appropriate model for both fast and slow moving endosomes. This article is part of a Special Issue entitled: “Recent Advances In Single-Particle Tracking: Experiment and Analysis” edited by Janusz Szwabiński and Aleksander Weron. MDPI 2021-07-27 /pmc/articles/PMC8394768/ /pubmed/34441098 http://dx.doi.org/10.3390/e23080958 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Korabel, Nickolay
Han, Daniel
Taloni, Alessandro
Pagnini, Gianni
Fedotov, Sergei
Allan, Viki
Waigh, Thomas Andrew
Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes
title Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes
title_full Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes
title_fullStr Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes
title_full_unstemmed Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes
title_short Local Analysis of Heterogeneous Intracellular Transport: Slow and Fast Moving Endosomes
title_sort local analysis of heterogeneous intracellular transport: slow and fast moving endosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394768/
https://www.ncbi.nlm.nih.gov/pubmed/34441098
http://dx.doi.org/10.3390/e23080958
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