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Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins

Zea mays L. is one of the most produced crops, and there are still parts of the world where maize is the basic staple food. To improve agriculture, mankind always looks for new, better methods of growing crops, especially in the current changing climatic conditions. Cold atmospheric pressure plasma...

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Autores principales: Holubová, Ľudmila, Švubová, Renáta, Slováková, Ľudmila, Bokor, Boris, Chobotová Kročková, Valéria, Renčko, Ján, Uhrin, Filip, Medvecká, Veronika, Zahoranová, Anna, Gálová, Eliška
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395187/
https://www.ncbi.nlm.nih.gov/pubmed/34445215
http://dx.doi.org/10.3390/ijms22168509
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author Holubová, Ľudmila
Švubová, Renáta
Slováková, Ľudmila
Bokor, Boris
Chobotová Kročková, Valéria
Renčko, Ján
Uhrin, Filip
Medvecká, Veronika
Zahoranová, Anna
Gálová, Eliška
author_facet Holubová, Ľudmila
Švubová, Renáta
Slováková, Ľudmila
Bokor, Boris
Chobotová Kročková, Valéria
Renčko, Ján
Uhrin, Filip
Medvecká, Veronika
Zahoranová, Anna
Gálová, Eliška
author_sort Holubová, Ľudmila
collection PubMed
description Zea mays L. is one of the most produced crops, and there are still parts of the world where maize is the basic staple food. To improve agriculture, mankind always looks for new, better methods of growing crops, especially in the current changing climatic conditions. Cold atmospheric pressure plasma (CAPP) has already showed its potential to enhance the culturing of crops, but it still needs more research for safe implementation into agriculture. In this work, it was shown that short CAPP treatment of maize grains had a positive effect on the vitality of grains and young seedlings, which may be connected to stimulation of antioxidant and lytic enzyme activities by short CAPP treatment. However, the prolonged treatment had a negative impact on the germination, growth, and production indexes. CAPP treatment caused the increased expression of genes for heat shock proteins HSP101 and HSP70 in the first two days after sowing. Using comet assay it was observed that shorter treatment times (30–120 s) did not cause DNA damage. Surface diagnostics of plasma-treated grains showed that plasma increases the hydrophilicity of the surface but does not damage the chemical bonds on the surface.
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spelling pubmed-83951872021-08-28 Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins Holubová, Ľudmila Švubová, Renáta Slováková, Ľudmila Bokor, Boris Chobotová Kročková, Valéria Renčko, Ján Uhrin, Filip Medvecká, Veronika Zahoranová, Anna Gálová, Eliška Int J Mol Sci Article Zea mays L. is one of the most produced crops, and there are still parts of the world where maize is the basic staple food. To improve agriculture, mankind always looks for new, better methods of growing crops, especially in the current changing climatic conditions. Cold atmospheric pressure plasma (CAPP) has already showed its potential to enhance the culturing of crops, but it still needs more research for safe implementation into agriculture. In this work, it was shown that short CAPP treatment of maize grains had a positive effect on the vitality of grains and young seedlings, which may be connected to stimulation of antioxidant and lytic enzyme activities by short CAPP treatment. However, the prolonged treatment had a negative impact on the germination, growth, and production indexes. CAPP treatment caused the increased expression of genes for heat shock proteins HSP101 and HSP70 in the first two days after sowing. Using comet assay it was observed that shorter treatment times (30–120 s) did not cause DNA damage. Surface diagnostics of plasma-treated grains showed that plasma increases the hydrophilicity of the surface but does not damage the chemical bonds on the surface. MDPI 2021-08-07 /pmc/articles/PMC8395187/ /pubmed/34445215 http://dx.doi.org/10.3390/ijms22168509 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Holubová, Ľudmila
Švubová, Renáta
Slováková, Ľudmila
Bokor, Boris
Chobotová Kročková, Valéria
Renčko, Ján
Uhrin, Filip
Medvecká, Veronika
Zahoranová, Anna
Gálová, Eliška
Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins
title Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins
title_full Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins
title_fullStr Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins
title_full_unstemmed Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins
title_short Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins
title_sort cold atmospheric pressure plasma treatment of maize grains—induction of growth, enzyme activities and heat shock proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395187/
https://www.ncbi.nlm.nih.gov/pubmed/34445215
http://dx.doi.org/10.3390/ijms22168509
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