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Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins
Zea mays L. is one of the most produced crops, and there are still parts of the world where maize is the basic staple food. To improve agriculture, mankind always looks for new, better methods of growing crops, especially in the current changing climatic conditions. Cold atmospheric pressure plasma...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395187/ https://www.ncbi.nlm.nih.gov/pubmed/34445215 http://dx.doi.org/10.3390/ijms22168509 |
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author | Holubová, Ľudmila Švubová, Renáta Slováková, Ľudmila Bokor, Boris Chobotová Kročková, Valéria Renčko, Ján Uhrin, Filip Medvecká, Veronika Zahoranová, Anna Gálová, Eliška |
author_facet | Holubová, Ľudmila Švubová, Renáta Slováková, Ľudmila Bokor, Boris Chobotová Kročková, Valéria Renčko, Ján Uhrin, Filip Medvecká, Veronika Zahoranová, Anna Gálová, Eliška |
author_sort | Holubová, Ľudmila |
collection | PubMed |
description | Zea mays L. is one of the most produced crops, and there are still parts of the world where maize is the basic staple food. To improve agriculture, mankind always looks for new, better methods of growing crops, especially in the current changing climatic conditions. Cold atmospheric pressure plasma (CAPP) has already showed its potential to enhance the culturing of crops, but it still needs more research for safe implementation into agriculture. In this work, it was shown that short CAPP treatment of maize grains had a positive effect on the vitality of grains and young seedlings, which may be connected to stimulation of antioxidant and lytic enzyme activities by short CAPP treatment. However, the prolonged treatment had a negative impact on the germination, growth, and production indexes. CAPP treatment caused the increased expression of genes for heat shock proteins HSP101 and HSP70 in the first two days after sowing. Using comet assay it was observed that shorter treatment times (30–120 s) did not cause DNA damage. Surface diagnostics of plasma-treated grains showed that plasma increases the hydrophilicity of the surface but does not damage the chemical bonds on the surface. |
format | Online Article Text |
id | pubmed-8395187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83951872021-08-28 Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins Holubová, Ľudmila Švubová, Renáta Slováková, Ľudmila Bokor, Boris Chobotová Kročková, Valéria Renčko, Ján Uhrin, Filip Medvecká, Veronika Zahoranová, Anna Gálová, Eliška Int J Mol Sci Article Zea mays L. is one of the most produced crops, and there are still parts of the world where maize is the basic staple food. To improve agriculture, mankind always looks for new, better methods of growing crops, especially in the current changing climatic conditions. Cold atmospheric pressure plasma (CAPP) has already showed its potential to enhance the culturing of crops, but it still needs more research for safe implementation into agriculture. In this work, it was shown that short CAPP treatment of maize grains had a positive effect on the vitality of grains and young seedlings, which may be connected to stimulation of antioxidant and lytic enzyme activities by short CAPP treatment. However, the prolonged treatment had a negative impact on the germination, growth, and production indexes. CAPP treatment caused the increased expression of genes for heat shock proteins HSP101 and HSP70 in the first two days after sowing. Using comet assay it was observed that shorter treatment times (30–120 s) did not cause DNA damage. Surface diagnostics of plasma-treated grains showed that plasma increases the hydrophilicity of the surface but does not damage the chemical bonds on the surface. MDPI 2021-08-07 /pmc/articles/PMC8395187/ /pubmed/34445215 http://dx.doi.org/10.3390/ijms22168509 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Holubová, Ľudmila Švubová, Renáta Slováková, Ľudmila Bokor, Boris Chobotová Kročková, Valéria Renčko, Ján Uhrin, Filip Medvecká, Veronika Zahoranová, Anna Gálová, Eliška Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins |
title | Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins |
title_full | Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins |
title_fullStr | Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins |
title_full_unstemmed | Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins |
title_short | Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins |
title_sort | cold atmospheric pressure plasma treatment of maize grains—induction of growth, enzyme activities and heat shock proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395187/ https://www.ncbi.nlm.nih.gov/pubmed/34445215 http://dx.doi.org/10.3390/ijms22168509 |
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