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SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response

UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved in these cellular mechanisms have been described, the entire repair cascade has remained unexplored. To...

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Autores principales: Majoros, Hajnalka, Borsos, Barbara N., Ujfaludi, Zsuzsanna, Páhi, Zoltán G., Mórocz, Mónika, Haracska, Lajos, Boros, Imre Miklós, Pankotai, Tibor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395218/
https://www.ncbi.nlm.nih.gov/pubmed/34445206
http://dx.doi.org/10.3390/ijms22168500
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author Majoros, Hajnalka
Borsos, Barbara N.
Ujfaludi, Zsuzsanna
Páhi, Zoltán G.
Mórocz, Mónika
Haracska, Lajos
Boros, Imre Miklós
Pankotai, Tibor
author_facet Majoros, Hajnalka
Borsos, Barbara N.
Ujfaludi, Zsuzsanna
Páhi, Zoltán G.
Mórocz, Mónika
Haracska, Lajos
Boros, Imre Miklós
Pankotai, Tibor
author_sort Majoros, Hajnalka
collection PubMed
description UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved in these cellular mechanisms have been described, the entire repair cascade has remained unexplored. To identify new players in UV-induced repair, we performed a microarray screen, in which we found SerpinB10 (SPB10, Bomapin) as one of the most dramatically upregulated genes following UV irradiation. Here, we demonstrated that an increased mRNA level of SPB10 is a general cellular response following UV irradiation regardless of the cell type. We showed that although SPB10 is implicated in the UV-induced cellular response, it has no indispensable function in cell survival upon UV irradiation. Nonetheless, we revealed that SPB10 might be involved in delaying the duration of DNA repair in interphase and also in S-phase cells. Additionally, we also highlighted the interaction between SPB10 and H3. Based on our results, it seems that SPB10 protein is implicated in UV-induced stress as a “quality control protein”, presumably by slowing down the repair process.
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spelling pubmed-83952182021-08-28 SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response Majoros, Hajnalka Borsos, Barbara N. Ujfaludi, Zsuzsanna Páhi, Zoltán G. Mórocz, Mónika Haracska, Lajos Boros, Imre Miklós Pankotai, Tibor Int J Mol Sci Article UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved in these cellular mechanisms have been described, the entire repair cascade has remained unexplored. To identify new players in UV-induced repair, we performed a microarray screen, in which we found SerpinB10 (SPB10, Bomapin) as one of the most dramatically upregulated genes following UV irradiation. Here, we demonstrated that an increased mRNA level of SPB10 is a general cellular response following UV irradiation regardless of the cell type. We showed that although SPB10 is implicated in the UV-induced cellular response, it has no indispensable function in cell survival upon UV irradiation. Nonetheless, we revealed that SPB10 might be involved in delaying the duration of DNA repair in interphase and also in S-phase cells. Additionally, we also highlighted the interaction between SPB10 and H3. Based on our results, it seems that SPB10 protein is implicated in UV-induced stress as a “quality control protein”, presumably by slowing down the repair process. MDPI 2021-08-07 /pmc/articles/PMC8395218/ /pubmed/34445206 http://dx.doi.org/10.3390/ijms22168500 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Majoros, Hajnalka
Borsos, Barbara N.
Ujfaludi, Zsuzsanna
Páhi, Zoltán G.
Mórocz, Mónika
Haracska, Lajos
Boros, Imre Miklós
Pankotai, Tibor
SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
title SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
title_full SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
title_fullStr SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
title_full_unstemmed SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
title_short SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response
title_sort serpinb10, a serine protease inhibitor, is implicated in uv-induced cellular response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395218/
https://www.ncbi.nlm.nih.gov/pubmed/34445206
http://dx.doi.org/10.3390/ijms22168500
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