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Specific and Global RNA Regulators in Pseudomonas aeruginosa
Pseudomonas aeruginosa (Pae) is an opportunistic pathogen showing a high intrinsic resistance to a wide variety of antibiotics. It causes nosocomial infections that are particularly detrimental to immunocompromised individuals and to patients suffering from cystic fibrosis. We provide a snapshot on...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395346/ https://www.ncbi.nlm.nih.gov/pubmed/34445336 http://dx.doi.org/10.3390/ijms22168632 |
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author | Pusic, Petra Sonnleitner, Elisabeth Bläsi, Udo |
author_facet | Pusic, Petra Sonnleitner, Elisabeth Bläsi, Udo |
author_sort | Pusic, Petra |
collection | PubMed |
description | Pseudomonas aeruginosa (Pae) is an opportunistic pathogen showing a high intrinsic resistance to a wide variety of antibiotics. It causes nosocomial infections that are particularly detrimental to immunocompromised individuals and to patients suffering from cystic fibrosis. We provide a snapshot on regulatory RNAs of Pae that impact on metabolism, pathogenicity and antibiotic susceptibility. Different experimental approaches such as in silico predictions, co-purification with the RNA chaperone Hfq as well as high-throughput RNA sequencing identified several hundreds of regulatory RNA candidates in Pae. Notwithstanding, using in vitro and in vivo assays, the function of only a few has been revealed. Here, we focus on well-characterized small base-pairing RNAs, regulating specific target genes as well as on larger protein-binding RNAs that sequester and thereby modulate the activity of translational repressors. As the latter impact large gene networks governing metabolism, acute or chronic infections, these protein-binding RNAs in conjunction with their cognate proteins are regarded as global post-transcriptional regulators. |
format | Online Article Text |
id | pubmed-8395346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83953462021-08-28 Specific and Global RNA Regulators in Pseudomonas aeruginosa Pusic, Petra Sonnleitner, Elisabeth Bläsi, Udo Int J Mol Sci Review Pseudomonas aeruginosa (Pae) is an opportunistic pathogen showing a high intrinsic resistance to a wide variety of antibiotics. It causes nosocomial infections that are particularly detrimental to immunocompromised individuals and to patients suffering from cystic fibrosis. We provide a snapshot on regulatory RNAs of Pae that impact on metabolism, pathogenicity and antibiotic susceptibility. Different experimental approaches such as in silico predictions, co-purification with the RNA chaperone Hfq as well as high-throughput RNA sequencing identified several hundreds of regulatory RNA candidates in Pae. Notwithstanding, using in vitro and in vivo assays, the function of only a few has been revealed. Here, we focus on well-characterized small base-pairing RNAs, regulating specific target genes as well as on larger protein-binding RNAs that sequester and thereby modulate the activity of translational repressors. As the latter impact large gene networks governing metabolism, acute or chronic infections, these protein-binding RNAs in conjunction with their cognate proteins are regarded as global post-transcriptional regulators. MDPI 2021-08-11 /pmc/articles/PMC8395346/ /pubmed/34445336 http://dx.doi.org/10.3390/ijms22168632 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pusic, Petra Sonnleitner, Elisabeth Bläsi, Udo Specific and Global RNA Regulators in Pseudomonas aeruginosa |
title | Specific and Global RNA Regulators in Pseudomonas aeruginosa |
title_full | Specific and Global RNA Regulators in Pseudomonas aeruginosa |
title_fullStr | Specific and Global RNA Regulators in Pseudomonas aeruginosa |
title_full_unstemmed | Specific and Global RNA Regulators in Pseudomonas aeruginosa |
title_short | Specific and Global RNA Regulators in Pseudomonas aeruginosa |
title_sort | specific and global rna regulators in pseudomonas aeruginosa |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395346/ https://www.ncbi.nlm.nih.gov/pubmed/34445336 http://dx.doi.org/10.3390/ijms22168632 |
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