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In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes

A normally functioning nervous system requires normal extracellular potassium ion concentration ([K]o). Throughout the nervous system, several processes, including those of an astrocytic nature, are involved in [K]o regulation. In this study we investigated the effect of astrocytic photostimulation...

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Autores principales: EbrahimAmini, Azin, Mylvaganam, Shanthini, Bazzigaluppi, Paolo, Khazaei, Mohamad, Velumian, Alexander, Stefanovic, Bojana, Carlen, Peter L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395460/
https://www.ncbi.nlm.nih.gov/pubmed/34445364
http://dx.doi.org/10.3390/ijms22168658
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author EbrahimAmini, Azin
Mylvaganam, Shanthini
Bazzigaluppi, Paolo
Khazaei, Mohamad
Velumian, Alexander
Stefanovic, Bojana
Carlen, Peter L.
author_facet EbrahimAmini, Azin
Mylvaganam, Shanthini
Bazzigaluppi, Paolo
Khazaei, Mohamad
Velumian, Alexander
Stefanovic, Bojana
Carlen, Peter L.
author_sort EbrahimAmini, Azin
collection PubMed
description A normally functioning nervous system requires normal extracellular potassium ion concentration ([K]o). Throughout the nervous system, several processes, including those of an astrocytic nature, are involved in [K]o regulation. In this study we investigated the effect of astrocytic photostimulation on [K]o. We hypothesized that in vivo photostimulation of eNpHR-expressing astrocytes leads to a decreased [K]o. Using optogenetic and electrophysiological techniques we showed that stimulation of eNpHR-expressing astrocytes resulted in a significantly decreased resting [K]o and evoked K responses. The amplitude of the concomitant spreading depolarization-like events also decreased. Our results imply that astrocytic membrane potential modification could be a potential tool for adjusting the [K]o.
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spelling pubmed-83954602021-08-28 In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes EbrahimAmini, Azin Mylvaganam, Shanthini Bazzigaluppi, Paolo Khazaei, Mohamad Velumian, Alexander Stefanovic, Bojana Carlen, Peter L. Int J Mol Sci Article A normally functioning nervous system requires normal extracellular potassium ion concentration ([K]o). Throughout the nervous system, several processes, including those of an astrocytic nature, are involved in [K]o regulation. In this study we investigated the effect of astrocytic photostimulation on [K]o. We hypothesized that in vivo photostimulation of eNpHR-expressing astrocytes leads to a decreased [K]o. Using optogenetic and electrophysiological techniques we showed that stimulation of eNpHR-expressing astrocytes resulted in a significantly decreased resting [K]o and evoked K responses. The amplitude of the concomitant spreading depolarization-like events also decreased. Our results imply that astrocytic membrane potential modification could be a potential tool for adjusting the [K]o. MDPI 2021-08-12 /pmc/articles/PMC8395460/ /pubmed/34445364 http://dx.doi.org/10.3390/ijms22168658 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
EbrahimAmini, Azin
Mylvaganam, Shanthini
Bazzigaluppi, Paolo
Khazaei, Mohamad
Velumian, Alexander
Stefanovic, Bojana
Carlen, Peter L.
In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes
title In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes
title_full In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes
title_fullStr In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes
title_full_unstemmed In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes
title_short In Vivo Neocortical [K]o Modulation by Targeted Stimulation of Astrocytes
title_sort in vivo neocortical [k]o modulation by targeted stimulation of astrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395460/
https://www.ncbi.nlm.nih.gov/pubmed/34445364
http://dx.doi.org/10.3390/ijms22168658
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