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Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns
Clostridium collagenase has provided superior clinical results in achieving digestion of immediate and accumulating devitalized collagen tissue. Recent studies suggest that debridement via Clostridium collagenase modulates a cellular response to foster an anti-inflammatory microenvironment milieu, a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395468/ https://www.ncbi.nlm.nih.gov/pubmed/34445347 http://dx.doi.org/10.3390/ijms22168643 |
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author | Frederick, Rosanne E. Bearden, Robert Jovanovic, Aleksa Jacobson, Nasreen Sood, Rajiv Dhall, Sandeep |
author_facet | Frederick, Rosanne E. Bearden, Robert Jovanovic, Aleksa Jacobson, Nasreen Sood, Rajiv Dhall, Sandeep |
author_sort | Frederick, Rosanne E. |
collection | PubMed |
description | Clostridium collagenase has provided superior clinical results in achieving digestion of immediate and accumulating devitalized collagen tissue. Recent studies suggest that debridement via Clostridium collagenase modulates a cellular response to foster an anti-inflammatory microenvironment milieu, allowing for a more coordinated healing response. In an effort to better understand its role in burn wounds, we evaluated Clostridium collagenase’s ability to effectively minimize burn progression using the classic burn comb model in pigs. Following burn injury, wounds were treated with Clostridium collagenase or control vehicle daily and biopsied at various time points. Biopsies were evaluated for factors associated with progressing necrosis as well as inflammatory response associated with treatment. Data presented herein showed that Clostridium collagenase treatment prevented destruction of dermal collagen. Additionally, treatment with collagenase reduced necrosis (HMGB1) and apoptosis (CC3a) early in burn injuries, allowing for increased infiltration of cells and protecting tissue from conversion. Furthermore, early epidermal separation and epidermal loss with a clearly defined basement membrane was observed in the treated wounds. We also show that collagenase treatment provided an early and improved inflammatory response followed by faster resolution in neutrophils. In assessing the inflammatory response, collagenase-treated wounds exhibited significantly greater neutrophil influx at day 1, with macrophage recruitment throughout days 2 and 4. In further evaluation, macrophage polarization to MHC II and vascular network maintenance were significantly increased in collagenase-treated wounds, indicative of a pro-resolving macrophage environment. Taken together, these data validate the impact of clostridial collagenases in the pathophysiology of burn wounds and that they complement patient outcomes in the clinical scenario. |
format | Online Article Text |
id | pubmed-8395468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83954682021-08-28 Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns Frederick, Rosanne E. Bearden, Robert Jovanovic, Aleksa Jacobson, Nasreen Sood, Rajiv Dhall, Sandeep Int J Mol Sci Article Clostridium collagenase has provided superior clinical results in achieving digestion of immediate and accumulating devitalized collagen tissue. Recent studies suggest that debridement via Clostridium collagenase modulates a cellular response to foster an anti-inflammatory microenvironment milieu, allowing for a more coordinated healing response. In an effort to better understand its role in burn wounds, we evaluated Clostridium collagenase’s ability to effectively minimize burn progression using the classic burn comb model in pigs. Following burn injury, wounds were treated with Clostridium collagenase or control vehicle daily and biopsied at various time points. Biopsies were evaluated for factors associated with progressing necrosis as well as inflammatory response associated with treatment. Data presented herein showed that Clostridium collagenase treatment prevented destruction of dermal collagen. Additionally, treatment with collagenase reduced necrosis (HMGB1) and apoptosis (CC3a) early in burn injuries, allowing for increased infiltration of cells and protecting tissue from conversion. Furthermore, early epidermal separation and epidermal loss with a clearly defined basement membrane was observed in the treated wounds. We also show that collagenase treatment provided an early and improved inflammatory response followed by faster resolution in neutrophils. In assessing the inflammatory response, collagenase-treated wounds exhibited significantly greater neutrophil influx at day 1, with macrophage recruitment throughout days 2 and 4. In further evaluation, macrophage polarization to MHC II and vascular network maintenance were significantly increased in collagenase-treated wounds, indicative of a pro-resolving macrophage environment. Taken together, these data validate the impact of clostridial collagenases in the pathophysiology of burn wounds and that they complement patient outcomes in the clinical scenario. MDPI 2021-08-11 /pmc/articles/PMC8395468/ /pubmed/34445347 http://dx.doi.org/10.3390/ijms22168643 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Frederick, Rosanne E. Bearden, Robert Jovanovic, Aleksa Jacobson, Nasreen Sood, Rajiv Dhall, Sandeep Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns |
title | Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns |
title_full | Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns |
title_fullStr | Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns |
title_full_unstemmed | Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns |
title_short | Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns |
title_sort | clostridium collagenase impact on zone of stasis stabilization and transition to healthy tissue in burns |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395468/ https://www.ncbi.nlm.nih.gov/pubmed/34445347 http://dx.doi.org/10.3390/ijms22168643 |
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