Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes

BACKGROUND: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data availab...

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Detalles Bibliográficos
Autores principales: Yang, Lei, Xing, Binglin, Li, Fen, Wang, Li Kui, Yuan, Linlin, Mbuji, Amosi Leonard, Peng, Zhengqiang, Malhat, Farag, Wu, Shaoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395580/
https://www.ncbi.nlm.nih.gov/pubmed/34513339
http://dx.doi.org/10.7717/peerj.12069
Descripción
Sumario:BACKGROUND: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce. METHODS: In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes. RESULTS: A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified.

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