Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes

BACKGROUND: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data availab...

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Autores principales: Yang, Lei, Xing, Binglin, Li, Fen, Wang, Li Kui, Yuan, Linlin, Mbuji, Amosi Leonard, Peng, Zhengqiang, Malhat, Farag, Wu, Shaoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395580/
https://www.ncbi.nlm.nih.gov/pubmed/34513339
http://dx.doi.org/10.7717/peerj.12069
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author Yang, Lei
Xing, Binglin
Li, Fen
Wang, Li Kui
Yuan, Linlin
Mbuji, Amosi Leonard
Peng, Zhengqiang
Malhat, Farag
Wu, Shaoying
author_facet Yang, Lei
Xing, Binglin
Li, Fen
Wang, Li Kui
Yuan, Linlin
Mbuji, Amosi Leonard
Peng, Zhengqiang
Malhat, Farag
Wu, Shaoying
author_sort Yang, Lei
collection PubMed
description BACKGROUND: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce. METHODS: In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes. RESULTS: A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified.
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spelling pubmed-83955802021-09-09 Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes Yang, Lei Xing, Binglin Li, Fen Wang, Li Kui Yuan, Linlin Mbuji, Amosi Leonard Peng, Zhengqiang Malhat, Farag Wu, Shaoying PeerJ Biochemistry BACKGROUND: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce. METHODS: In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes. RESULTS: A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified. PeerJ Inc. 2021-08-24 /pmc/articles/PMC8395580/ /pubmed/34513339 http://dx.doi.org/10.7717/peerj.12069 Text en © 2021 Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Yang, Lei
Xing, Binglin
Li, Fen
Wang, Li Kui
Yuan, Linlin
Mbuji, Amosi Leonard
Peng, Zhengqiang
Malhat, Farag
Wu, Shaoying
Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
title Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
title_full Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
title_fullStr Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
title_full_unstemmed Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
title_short Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
title_sort full-length transcriptome analysis of spodoptera frugiperda larval brain reveals detoxification genes
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395580/
https://www.ncbi.nlm.nih.gov/pubmed/34513339
http://dx.doi.org/10.7717/peerj.12069
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