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An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics

Background: Pulmonary artery denervation (PADN) is an evolving interventional procedure capable to reduce pulmonary artery (PA) pressure. We aimed to compare PA nerve distribution in different specimens and assess the feasibility of an ovine model for a denervation procedure and evaluate the acute c...

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Autores principales: Condori Leandro, Heber Ivan, Koshevaya, Elena G., Mitrofanova, Lubov B., Vakhrushev, Aleksandr D., Goncharova, Natalia S., Korobchenko, Lev E., Andreeva, Elizaveta M., Lebedev, Dmitry S., Mikhaylov, Evgeny N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395814/
https://www.ncbi.nlm.nih.gov/pubmed/34445490
http://dx.doi.org/10.3390/ijms22168788
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author Condori Leandro, Heber Ivan
Koshevaya, Elena G.
Mitrofanova, Lubov B.
Vakhrushev, Aleksandr D.
Goncharova, Natalia S.
Korobchenko, Lev E.
Andreeva, Elizaveta M.
Lebedev, Dmitry S.
Mikhaylov, Evgeny N.
author_facet Condori Leandro, Heber Ivan
Koshevaya, Elena G.
Mitrofanova, Lubov B.
Vakhrushev, Aleksandr D.
Goncharova, Natalia S.
Korobchenko, Lev E.
Andreeva, Elizaveta M.
Lebedev, Dmitry S.
Mikhaylov, Evgeny N.
author_sort Condori Leandro, Heber Ivan
collection PubMed
description Background: Pulmonary artery denervation (PADN) is an evolving interventional procedure capable to reduce pulmonary artery (PA) pressure. We aimed to compare PA nerve distribution in different specimens and assess the feasibility of an ovine model for a denervation procedure and evaluate the acute changes induced by laser energy. Methods: The experiment was divided into two phases: (1) the analysis of PA nerve distribution in sheep, pigs, and humans using histological and immunochemical methods; (2) fiberoptic PADN in sheep and postmortem laser lesion characteristics. Results: PA nerve density and distribution in sheep differ from humans, although pigs and sheep share similar characteristics, nerve fibers are observed in the media layer, adventitia, and perivascular tissue in sheep. Necrosis of the intima and focal hemorrhages within the media, adventitia, and perivascular adipose tissue were evidenced post laser PADN. Among the identified lesions, 40% reached adventitia and could be classified as effective for PADN. The use of 20 W ablation energy was safer and 30 W-ablation led to collateral organ damage. Conclusions: An ovine model is suitable for PADN procedures; however, nerve distribution in the PA bifurcation and main branches differ from human PA innervation. Laser ablation can be safely used for PADN procedures.
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spelling pubmed-83958142021-08-28 An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics Condori Leandro, Heber Ivan Koshevaya, Elena G. Mitrofanova, Lubov B. Vakhrushev, Aleksandr D. Goncharova, Natalia S. Korobchenko, Lev E. Andreeva, Elizaveta M. Lebedev, Dmitry S. Mikhaylov, Evgeny N. Int J Mol Sci Article Background: Pulmonary artery denervation (PADN) is an evolving interventional procedure capable to reduce pulmonary artery (PA) pressure. We aimed to compare PA nerve distribution in different specimens and assess the feasibility of an ovine model for a denervation procedure and evaluate the acute changes induced by laser energy. Methods: The experiment was divided into two phases: (1) the analysis of PA nerve distribution in sheep, pigs, and humans using histological and immunochemical methods; (2) fiberoptic PADN in sheep and postmortem laser lesion characteristics. Results: PA nerve density and distribution in sheep differ from humans, although pigs and sheep share similar characteristics, nerve fibers are observed in the media layer, adventitia, and perivascular tissue in sheep. Necrosis of the intima and focal hemorrhages within the media, adventitia, and perivascular adipose tissue were evidenced post laser PADN. Among the identified lesions, 40% reached adventitia and could be classified as effective for PADN. The use of 20 W ablation energy was safer and 30 W-ablation led to collateral organ damage. Conclusions: An ovine model is suitable for PADN procedures; however, nerve distribution in the PA bifurcation and main branches differ from human PA innervation. Laser ablation can be safely used for PADN procedures. MDPI 2021-08-16 /pmc/articles/PMC8395814/ /pubmed/34445490 http://dx.doi.org/10.3390/ijms22168788 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Condori Leandro, Heber Ivan
Koshevaya, Elena G.
Mitrofanova, Lubov B.
Vakhrushev, Aleksandr D.
Goncharova, Natalia S.
Korobchenko, Lev E.
Andreeva, Elizaveta M.
Lebedev, Dmitry S.
Mikhaylov, Evgeny N.
An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics
title An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics
title_full An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics
title_fullStr An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics
title_full_unstemmed An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics
title_short An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics
title_sort ovine model for percutaneous pulmonary artery laser denervation: perivascular innervation and ablation lesion characteristics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395814/
https://www.ncbi.nlm.nih.gov/pubmed/34445490
http://dx.doi.org/10.3390/ijms22168788
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