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ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice

Spermatogenesis is a complicated process involving mitotically proliferating spermatogonial cells, meiotically dividing spermatocytes, and spermatid going through maturation into spermatozoa. The post-translational modifications of proteins play important roles in this biological process. S-palmitoy...

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Detalles Bibliográficos
Autores principales: Wang, Shuai, Qiao, Hongjie, Wang, Pengxiang, Wang, Yuan, Qin, Danian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396176/
https://www.ncbi.nlm.nih.gov/pubmed/34445597
http://dx.doi.org/10.3390/ijms22168894
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author Wang, Shuai
Qiao, Hongjie
Wang, Pengxiang
Wang, Yuan
Qin, Danian
author_facet Wang, Shuai
Qiao, Hongjie
Wang, Pengxiang
Wang, Yuan
Qin, Danian
author_sort Wang, Shuai
collection PubMed
description Spermatogenesis is a complicated process involving mitotically proliferating spermatogonial cells, meiotically dividing spermatocytes, and spermatid going through maturation into spermatozoa. The post-translational modifications of proteins play important roles in this biological process. S-palmitoylation is one type of protein modifications catalyzed by zinc finger Asp-His-His-Cys (ZDHHC)-family palmitoyl S-acyltransferases. There are 23 mammalian ZDHHCs that have been identified in mouse. Among them, Zdhhc19 is highly expressed in adult testis. However, the in vivo function of Zdhhc19 in mouse spermatogenesis and fertility remains unknown. In this study, we knocked out the Zdhhc19 gene by generating a 2609 bp deletion from exon 3 to exon 6 in mice. No differences were found in testis morphology and testis/body weight ratios upon Zdhhc19 deletion. Spermatogenesis was not disrupted in Zdhhc19 knockout mice, in which properly developed TRA98+ germ cells, SYCP3+ spermatocytes, and TNP1+ spermatids/spermatozoa were detected in seminiferous tubules. Nevertheless, Zdhhc19 knockout mice were male infertile. Zdhhc19 deficient spermatozoa exhibited multiple defects including abnormal morphology of sperm tails and heads, decreased motility, and disturbed acrosome reaction. All of these led to the inability of Zdhhc19 mutant sperm to fertilize oocytes in IVF assays. Taken together, our results support the fact that Zdhhc19 is a testis enriched gene dispensable for spermatogenesis, but is essential for sperm functions in mice.
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spelling pubmed-83961762021-08-28 ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice Wang, Shuai Qiao, Hongjie Wang, Pengxiang Wang, Yuan Qin, Danian Int J Mol Sci Article Spermatogenesis is a complicated process involving mitotically proliferating spermatogonial cells, meiotically dividing spermatocytes, and spermatid going through maturation into spermatozoa. The post-translational modifications of proteins play important roles in this biological process. S-palmitoylation is one type of protein modifications catalyzed by zinc finger Asp-His-His-Cys (ZDHHC)-family palmitoyl S-acyltransferases. There are 23 mammalian ZDHHCs that have been identified in mouse. Among them, Zdhhc19 is highly expressed in adult testis. However, the in vivo function of Zdhhc19 in mouse spermatogenesis and fertility remains unknown. In this study, we knocked out the Zdhhc19 gene by generating a 2609 bp deletion from exon 3 to exon 6 in mice. No differences were found in testis morphology and testis/body weight ratios upon Zdhhc19 deletion. Spermatogenesis was not disrupted in Zdhhc19 knockout mice, in which properly developed TRA98+ germ cells, SYCP3+ spermatocytes, and TNP1+ spermatids/spermatozoa were detected in seminiferous tubules. Nevertheless, Zdhhc19 knockout mice were male infertile. Zdhhc19 deficient spermatozoa exhibited multiple defects including abnormal morphology of sperm tails and heads, decreased motility, and disturbed acrosome reaction. All of these led to the inability of Zdhhc19 mutant sperm to fertilize oocytes in IVF assays. Taken together, our results support the fact that Zdhhc19 is a testis enriched gene dispensable for spermatogenesis, but is essential for sperm functions in mice. MDPI 2021-08-18 /pmc/articles/PMC8396176/ /pubmed/34445597 http://dx.doi.org/10.3390/ijms22168894 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Shuai
Qiao, Hongjie
Wang, Pengxiang
Wang, Yuan
Qin, Danian
ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice
title ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice
title_full ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice
title_fullStr ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice
title_full_unstemmed ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice
title_short ZDHHC19 Is Dispensable for Spermatogenesis, but Is Essential for Sperm Functions in Mice
title_sort zdhhc19 is dispensable for spermatogenesis, but is essential for sperm functions in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396176/
https://www.ncbi.nlm.nih.gov/pubmed/34445597
http://dx.doi.org/10.3390/ijms22168894
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