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Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing

Dysregulation of gap junction intercellular communication (GJIC) is recognized as one of the key hallmarks for identifying non-genotoxic carcinogens (NGTxC). Currently, there is a demand for in vitro assays addressing the gap junction hallmark, which would have the potential to eventually become an...

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Autores principales: Sovadinová, Iva, Upham, Brad L., Trosko, James E., Babica, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396440/
https://www.ncbi.nlm.nih.gov/pubmed/34445682
http://dx.doi.org/10.3390/ijms22168977
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author Sovadinová, Iva
Upham, Brad L.
Trosko, James E.
Babica, Pavel
author_facet Sovadinová, Iva
Upham, Brad L.
Trosko, James E.
Babica, Pavel
author_sort Sovadinová, Iva
collection PubMed
description Dysregulation of gap junction intercellular communication (GJIC) is recognized as one of the key hallmarks for identifying non-genotoxic carcinogens (NGTxC). Currently, there is a demand for in vitro assays addressing the gap junction hallmark, which would have the potential to eventually become an integral part of an integrated approach to the testing and assessment (IATA) of NGTxC. The scrape loading-dye transfer (SL-DT) technique is a simple assay for the functional evaluation of GJIC in various in vitro cultured mammalian cells and represents an interesting candidate assay. Out of the various techniques for evaluating GJIC, the SL-DT assay has been used frequently to assess the effects of various chemicals on GJIC in toxicological and tumor promotion research. In this review, we systematically searched the existing literature to gather papers assessing GJIC using the SL-DT assay in a rat liver epithelial cell line, WB-F344, after treating with chemicals, especially environmental and food toxicants, drugs, reproductive-, cardio- and neuro-toxicants and chemical tumor promoters. We discuss findings derived from the SL-DT assay with the known knowledge about the tumor-promoting activity and carcinogenicity of the assessed chemicals to evaluate the predictive capacity of the SL-DT assay in terms of its sensitivity, specificity and accuracy for identifying carcinogens. These data represent important information with respect to the applicability of the SL-DT assay for the testing of NGTxC within the IATA framework.
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spelling pubmed-83964402021-08-28 Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing Sovadinová, Iva Upham, Brad L. Trosko, James E. Babica, Pavel Int J Mol Sci Review Dysregulation of gap junction intercellular communication (GJIC) is recognized as one of the key hallmarks for identifying non-genotoxic carcinogens (NGTxC). Currently, there is a demand for in vitro assays addressing the gap junction hallmark, which would have the potential to eventually become an integral part of an integrated approach to the testing and assessment (IATA) of NGTxC. The scrape loading-dye transfer (SL-DT) technique is a simple assay for the functional evaluation of GJIC in various in vitro cultured mammalian cells and represents an interesting candidate assay. Out of the various techniques for evaluating GJIC, the SL-DT assay has been used frequently to assess the effects of various chemicals on GJIC in toxicological and tumor promotion research. In this review, we systematically searched the existing literature to gather papers assessing GJIC using the SL-DT assay in a rat liver epithelial cell line, WB-F344, after treating with chemicals, especially environmental and food toxicants, drugs, reproductive-, cardio- and neuro-toxicants and chemical tumor promoters. We discuss findings derived from the SL-DT assay with the known knowledge about the tumor-promoting activity and carcinogenicity of the assessed chemicals to evaluate the predictive capacity of the SL-DT assay in terms of its sensitivity, specificity and accuracy for identifying carcinogens. These data represent important information with respect to the applicability of the SL-DT assay for the testing of NGTxC within the IATA framework. MDPI 2021-08-20 /pmc/articles/PMC8396440/ /pubmed/34445682 http://dx.doi.org/10.3390/ijms22168977 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Sovadinová, Iva
Upham, Brad L.
Trosko, James E.
Babica, Pavel
Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing
title Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing
title_full Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing
title_fullStr Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing
title_full_unstemmed Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing
title_short Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing
title_sort applicability of scrape loading-dye transfer assay for non-genotoxic carcinogen testing
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396440/
https://www.ncbi.nlm.nih.gov/pubmed/34445682
http://dx.doi.org/10.3390/ijms22168977
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