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Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants
Despite their notable root mutualism with blueberries (Vaccinium spp.), studies related to Ericoid mycorrhizal (ERM) are relatively limited. In this study, we report the isolation of 14 endomycorrhizal fungi and their identification by fungal colony morphology characterization combined with PCR-ampl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396871/ https://www.ncbi.nlm.nih.gov/pubmed/34436123 http://dx.doi.org/10.3390/jof7080584 |
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author | Cai, Binbin Vancov, Tony Si, Hanqi Yang, Wenru Tong, Kunning Chen, Wenrong Fang, Yunying |
author_facet | Cai, Binbin Vancov, Tony Si, Hanqi Yang, Wenru Tong, Kunning Chen, Wenrong Fang, Yunying |
author_sort | Cai, Binbin |
collection | PubMed |
description | Despite their notable root mutualism with blueberries (Vaccinium spp.), studies related to Ericoid mycorrhizal (ERM) are relatively limited. In this study, we report the isolation of 14 endomycorrhizal fungi and their identification by fungal colony morphology characterization combined with PCR-amplified fungal internal transcribed spacer (ITS) sequence analyses. Six of the isolated strains were confirmed as beneficial mycorrhizal fungi for blueberry plants following inoculation. We observed the formation of typical ERM hyphae coil structures—which promote and nutritionally support growth—in blueberry seedlings and significant nitrogen and phosphorous content increases in diverse tissues. QRT-PCRs confirmed changes in VcPHT1s expression patterns. After the formation of ERM, PHT1-1 transcription in roots was upregulated by 1.4- to threefold, whilst expression of PHT1-3 and PHT1-4 in roots were downregulated 72% and 60%, respectively. Amino acid sequence analysis of all four VcPHT1s genes from the blueberry variety “Sharpblue” revealed an overall structural similarity of 67% and predicted transmembrane domains. Cloning and overexpression of PHT1-1 and PHT1-3 genes in transgenic Arabidopsis thaliana plants significantly enriched total phosphorus and chlorophyll content, confirming that PHT1-1 and PHT1-3 gene functions are associated with the transport and absorption of phosphorus. |
format | Online Article Text |
id | pubmed-8396871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83968712021-08-28 Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants Cai, Binbin Vancov, Tony Si, Hanqi Yang, Wenru Tong, Kunning Chen, Wenrong Fang, Yunying J Fungi (Basel) Article Despite their notable root mutualism with blueberries (Vaccinium spp.), studies related to Ericoid mycorrhizal (ERM) are relatively limited. In this study, we report the isolation of 14 endomycorrhizal fungi and their identification by fungal colony morphology characterization combined with PCR-amplified fungal internal transcribed spacer (ITS) sequence analyses. Six of the isolated strains were confirmed as beneficial mycorrhizal fungi for blueberry plants following inoculation. We observed the formation of typical ERM hyphae coil structures—which promote and nutritionally support growth—in blueberry seedlings and significant nitrogen and phosphorous content increases in diverse tissues. QRT-PCRs confirmed changes in VcPHT1s expression patterns. After the formation of ERM, PHT1-1 transcription in roots was upregulated by 1.4- to threefold, whilst expression of PHT1-3 and PHT1-4 in roots were downregulated 72% and 60%, respectively. Amino acid sequence analysis of all four VcPHT1s genes from the blueberry variety “Sharpblue” revealed an overall structural similarity of 67% and predicted transmembrane domains. Cloning and overexpression of PHT1-1 and PHT1-3 genes in transgenic Arabidopsis thaliana plants significantly enriched total phosphorus and chlorophyll content, confirming that PHT1-1 and PHT1-3 gene functions are associated with the transport and absorption of phosphorus. MDPI 2021-07-21 /pmc/articles/PMC8396871/ /pubmed/34436123 http://dx.doi.org/10.3390/jof7080584 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cai, Binbin Vancov, Tony Si, Hanqi Yang, Wenru Tong, Kunning Chen, Wenrong Fang, Yunying Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants |
title | Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants |
title_full | Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants |
title_fullStr | Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants |
title_full_unstemmed | Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants |
title_short | Isolation and Characterization of Endomycorrhizal Fungi Associated with Growth Promotion of Blueberry Plants |
title_sort | isolation and characterization of endomycorrhizal fungi associated with growth promotion of blueberry plants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396871/ https://www.ncbi.nlm.nih.gov/pubmed/34436123 http://dx.doi.org/10.3390/jof7080584 |
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