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A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex

In recent years, considerable advances have been made in clearing up the phylogenetic relationships within the Arthrodermataceae family. However, certain closely related taxa still contain poorly resolved species boundaries. Here, we tried to elucidate the species composition of the Trichophyton ben...

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Autores principales: Baert, Frederik, Lefevere, Paulien, D’hooge, Elizabet, Stubbe, Dirk, Packeu, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397008/
https://www.ncbi.nlm.nih.gov/pubmed/34436141
http://dx.doi.org/10.3390/jof7080602
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author Baert, Frederik
Lefevere, Paulien
D’hooge, Elizabet
Stubbe, Dirk
Packeu, Ann
author_facet Baert, Frederik
Lefevere, Paulien
D’hooge, Elizabet
Stubbe, Dirk
Packeu, Ann
author_sort Baert, Frederik
collection PubMed
description In recent years, considerable advances have been made in clearing up the phylogenetic relationships within the Arthrodermataceae family. However, certain closely related taxa still contain poorly resolved species boundaries. Here, we tried to elucidate the species composition of the Trichophyton benhamiae species complex using a combined approach consisting of multi-gene phylogenetic analysis based on internal transcribed spacer (ITS) and beta-tubulin (BT) gene regions, morphological analysis, and spectral comparison using MALDI-ToF. We confirmed the existence of 11 different monophyletic clades within the complex representing either species or genetically distinct groups within species. MALDI-ToF spectrometry analysis revealed that most of these clades were readily distinguishable from one another; however, some closely related sister clades, such as T. europaeum and T. japonicum, were often misidentified as their counterpart. The distinct “yellow” and “white” phenotypes of T. benhamiae do not have a clear genetic basis and should thus be considered as different morphotypes of the same species. Strains traditionally considered T. benhamiae can be divided into three main clades: (i) T. benhamiae, (ii) T. europaeum/T. japonicum, and (iii) the phylogenetically distant T. africanum. While T. europaeum and T. japonicum are distinguishable based on their genotype, spectral and morphological analysis did not provide clear delimiting characteristics.
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spelling pubmed-83970082021-08-28 A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex Baert, Frederik Lefevere, Paulien D’hooge, Elizabet Stubbe, Dirk Packeu, Ann J Fungi (Basel) Article In recent years, considerable advances have been made in clearing up the phylogenetic relationships within the Arthrodermataceae family. However, certain closely related taxa still contain poorly resolved species boundaries. Here, we tried to elucidate the species composition of the Trichophyton benhamiae species complex using a combined approach consisting of multi-gene phylogenetic analysis based on internal transcribed spacer (ITS) and beta-tubulin (BT) gene regions, morphological analysis, and spectral comparison using MALDI-ToF. We confirmed the existence of 11 different monophyletic clades within the complex representing either species or genetically distinct groups within species. MALDI-ToF spectrometry analysis revealed that most of these clades were readily distinguishable from one another; however, some closely related sister clades, such as T. europaeum and T. japonicum, were often misidentified as their counterpart. The distinct “yellow” and “white” phenotypes of T. benhamiae do not have a clear genetic basis and should thus be considered as different morphotypes of the same species. Strains traditionally considered T. benhamiae can be divided into three main clades: (i) T. benhamiae, (ii) T. europaeum/T. japonicum, and (iii) the phylogenetically distant T. africanum. While T. europaeum and T. japonicum are distinguishable based on their genotype, spectral and morphological analysis did not provide clear delimiting characteristics. MDPI 2021-07-26 /pmc/articles/PMC8397008/ /pubmed/34436141 http://dx.doi.org/10.3390/jof7080602 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baert, Frederik
Lefevere, Paulien
D’hooge, Elizabet
Stubbe, Dirk
Packeu, Ann
A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex
title A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex
title_full A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex
title_fullStr A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex
title_full_unstemmed A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex
title_short A Polyphasic Approach to Classification and Identification of Species within the Trichophyton benhamiae Complex
title_sort polyphasic approach to classification and identification of species within the trichophyton benhamiae complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397008/
https://www.ncbi.nlm.nih.gov/pubmed/34436141
http://dx.doi.org/10.3390/jof7080602
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