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Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae

SIMPLE SUMMARY: The two-spotted spider mite, Tetranychus urticae, is an important agricultural pest. Gene expression dynamics during different development time points of deutonymphal development process in T. urticae were investigated. The transcriptome sequencing aims to identify hormone- and cutic...

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Autores principales: Li, Gang, Gu, Xinyao, Gui, Shunhua, Guo, Jianjun, Yi, Tianci, Jin, Daochao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397179/
https://www.ncbi.nlm.nih.gov/pubmed/34442302
http://dx.doi.org/10.3390/insects12080736
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author Li, Gang
Gu, Xinyao
Gui, Shunhua
Guo, Jianjun
Yi, Tianci
Jin, Daochao
author_facet Li, Gang
Gu, Xinyao
Gui, Shunhua
Guo, Jianjun
Yi, Tianci
Jin, Daochao
author_sort Li, Gang
collection PubMed
description SIMPLE SUMMARY: The two-spotted spider mite, Tetranychus urticae, is an important agricultural pest. Gene expression dynamics during different development time points of deutonymphal development process in T. urticae were investigated. The transcriptome sequencing aims to identify hormone- and cuticle-related genes that regulated the development process of spider mite. Moreover, this comprehensive transcriptome will provide a foundation for further research of development process in T. urticae. ABSTRACT: Tetranychus urticae is an important agricultural pest that feeds on more than 1100 plant species. To investigate gene expression network in development process of deutonymph, a comprehensive transcriptome analysis of different developmental time points of deutonymph in T. urticae was performed. Comparing with expression profile of 7 h, 309, 876, 2736, and 3432 differential expression genes were detected at time points 14 h, 21 h, 28 h, and 35 h, respectively. The expression dynamic analysis indicated that genes in hormone- (ecdysteroid and juvenile hormone) and cuticle- (chitin and cuticle proteins) related pathways were indispensable for development process in deutonymph. Among hormone related pathway genes, the ecdysteroid biosynthesis pathway genes were highly expressed at the growth period of development process, which is opposite to the expression patterns of juvenile hormone biosynthesis pathway genes. For cuticle related pathway genes, 13 chitinase genes were identified in the genome of T. urticae, and 8 chitinase genes were highly expressed in different time points of developmental process in the deutonymph of T. urticae. Additionally, 59 cuticle protein genes were identified from genome, and most of the cuticle protein genes were expressed in the molting period of developmental process in deutonymph. This study reveals critical genes involved in the development process of deutonymph and also provides comprehensive development transcriptome information for finding more molecular targets to control this pest.
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spelling pubmed-83971792021-08-28 Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae Li, Gang Gu, Xinyao Gui, Shunhua Guo, Jianjun Yi, Tianci Jin, Daochao Insects Article SIMPLE SUMMARY: The two-spotted spider mite, Tetranychus urticae, is an important agricultural pest. Gene expression dynamics during different development time points of deutonymphal development process in T. urticae were investigated. The transcriptome sequencing aims to identify hormone- and cuticle-related genes that regulated the development process of spider mite. Moreover, this comprehensive transcriptome will provide a foundation for further research of development process in T. urticae. ABSTRACT: Tetranychus urticae is an important agricultural pest that feeds on more than 1100 plant species. To investigate gene expression network in development process of deutonymph, a comprehensive transcriptome analysis of different developmental time points of deutonymph in T. urticae was performed. Comparing with expression profile of 7 h, 309, 876, 2736, and 3432 differential expression genes were detected at time points 14 h, 21 h, 28 h, and 35 h, respectively. The expression dynamic analysis indicated that genes in hormone- (ecdysteroid and juvenile hormone) and cuticle- (chitin and cuticle proteins) related pathways were indispensable for development process in deutonymph. Among hormone related pathway genes, the ecdysteroid biosynthesis pathway genes were highly expressed at the growth period of development process, which is opposite to the expression patterns of juvenile hormone biosynthesis pathway genes. For cuticle related pathway genes, 13 chitinase genes were identified in the genome of T. urticae, and 8 chitinase genes were highly expressed in different time points of developmental process in the deutonymph of T. urticae. Additionally, 59 cuticle protein genes were identified from genome, and most of the cuticle protein genes were expressed in the molting period of developmental process in deutonymph. This study reveals critical genes involved in the development process of deutonymph and also provides comprehensive development transcriptome information for finding more molecular targets to control this pest. MDPI 2021-08-17 /pmc/articles/PMC8397179/ /pubmed/34442302 http://dx.doi.org/10.3390/insects12080736 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Gang
Gu, Xinyao
Gui, Shunhua
Guo, Jianjun
Yi, Tianci
Jin, Daochao
Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae
title Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae
title_full Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae
title_fullStr Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae
title_full_unstemmed Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae
title_short Transcriptome Analysis of Hormone-and Cuticle-Related Genes in the Development Process of Deutonymph in Tetranychus urticae
title_sort transcriptome analysis of hormone-and cuticle-related genes in the development process of deutonymph in tetranychus urticae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397179/
https://www.ncbi.nlm.nih.gov/pubmed/34442302
http://dx.doi.org/10.3390/insects12080736
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