Study of Morphological Features in Pre-Imaginal Honey Bee Impaired by Varroa destructor by Means of Computer Tomography

SIMPLE SUMMARY: The Varroa mite (Varroa destructor) is the most important natural pest of the honey bee, Apis mellifera, worldwide. The extent to which impairments in honey bees occur concomitantly upon infestation by this parasite greatly varies. Inter alia, the Varroa mite causes developmental disorders mediated by deformed wing virus in this host. Although there is a plethora of information regarding the consequences of this parasitism in the fully developed stage, data concerning the pre-imaginal honey bee stage inside the comb are rather scarce. In this study, morphological differences in the main body parts of the honey bee during the development stages of both intact and parasitized larvae were measured inside the comb by means of computed tomography. The images obtained reveal a visualization of the harmful effects of the Varroa mite on the pre-imaginal host. Our results demonstrate that the deformation of certain body parts was due to the presence of the parasite. Deformity, as the most conspicuous sign of infestation, is coupled with a decrease in the total-body size and abdomen size together with a disproportionate ratio of different body parts. In summary, information on the impairment of honey bee development triggered by the Varroa mite gives the opportunity to assess the damage caused by this serious pest, which occurs latently in honey bees. ABSTRACT: The honey bee (Apis mellifera L. 1778) is an essential element in maintaining the diversity of the biosphere and food production. One of its most important parasites is Varroa destructor, Anderson and Trueman, 2000, which plays a role in the vectoring of deformed wing virus (DWV) in honey bee colonies. Our aim was to measure the potential morphometric changes in the pre-imaginal stage of A. mellifera caused by varroosis by means of computed tomography, hence supplying evidence for the presumable role that V. destructor plays as a virus vector. Based on our results, the developmental disorders in honey bees that ensued during the pre-imaginal stages were evident. The total-body length and abdomen length of parasitized specimens were shorter than those of their intact companions. In addition, the calculated quotients of the total-body/abdomen, head/thorax, and head/abdomen in parasitized samples were significantly altered upon infestation. In our view, these phenotypical disorders can also be traced to viral infection mediated by parasitism, which was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. Capitalizing on a non-destructive method, our study reveals the deformation of the honey bee due to mite parasitism and the intermediary role this pest plays in viral infection, inside the brood cell.