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TiO(2) Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose
[Image: see text] Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors. A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat. Hydrot...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397235/ https://www.ncbi.nlm.nih.gov/pubmed/34340308 http://dx.doi.org/10.1021/acsami.1c11446 |
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author | Gunatilake, Udara Bimendra Garcia-Rey, Sandra Ojeda, Edilberto Basabe-Desmonts, Lourdes Benito-Lopez, Fernando |
author_facet | Gunatilake, Udara Bimendra Garcia-Rey, Sandra Ojeda, Edilberto Basabe-Desmonts, Lourdes Benito-Lopez, Fernando |
author_sort | Gunatilake, Udara Bimendra |
collection | PubMed |
description | [Image: see text] Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors. A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat. Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform. Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively. The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times. Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed. The properties of this new composite provide new avenues in the development of paper-based sensor devices. The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices. |
format | Online Article Text |
id | pubmed-8397235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-83972352021-08-31 TiO(2) Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose Gunatilake, Udara Bimendra Garcia-Rey, Sandra Ojeda, Edilberto Basabe-Desmonts, Lourdes Benito-Lopez, Fernando ACS Appl Mater Interfaces [Image: see text] Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors. A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat. Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform. Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively. The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times. Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed. The properties of this new composite provide new avenues in the development of paper-based sensor devices. The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices. American Chemical Society 2021-08-02 2021-08-11 /pmc/articles/PMC8397235/ /pubmed/34340308 http://dx.doi.org/10.1021/acsami.1c11446 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Gunatilake, Udara Bimendra Garcia-Rey, Sandra Ojeda, Edilberto Basabe-Desmonts, Lourdes Benito-Lopez, Fernando TiO(2) Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose |
title | TiO(2) Nanotubes Alginate Hydrogel Scaffold
for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose |
title_full | TiO(2) Nanotubes Alginate Hydrogel Scaffold
for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose |
title_fullStr | TiO(2) Nanotubes Alginate Hydrogel Scaffold
for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose |
title_full_unstemmed | TiO(2) Nanotubes Alginate Hydrogel Scaffold
for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose |
title_short | TiO(2) Nanotubes Alginate Hydrogel Scaffold
for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose |
title_sort | tio(2) nanotubes alginate hydrogel scaffold
for rapid sensing of sweat biomarkers: lactate and glucose |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397235/ https://www.ncbi.nlm.nih.gov/pubmed/34340308 http://dx.doi.org/10.1021/acsami.1c11446 |
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