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Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes

[Image: see text] Dried blood spot (DBS) metabolite analysis is a central tool for the clinic, e.g., newborn screening. Instead of applying multiple analytical methods, a single liquid chromatography-mass spectrometry (LC–MS) method was developed for metabolites spanning from highly polar glucose to...

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Autores principales: Skogvold, Hanne Bendiksen, Sandås, Elise Mørk, Østeby, Anja, Løkken, Camilla, Rootwelt, Helge, Rønning, Per Ola, Wilson, Steven Ray, Elgstøen, Katja Benedikte Prestø
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397434/
https://www.ncbi.nlm.nih.gov/pubmed/34296888
http://dx.doi.org/10.1021/acs.jproteome.1c00326
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author Skogvold, Hanne Bendiksen
Sandås, Elise Mørk
Østeby, Anja
Løkken, Camilla
Rootwelt, Helge
Rønning, Per Ola
Wilson, Steven Ray
Elgstøen, Katja Benedikte Prestø
author_facet Skogvold, Hanne Bendiksen
Sandås, Elise Mørk
Østeby, Anja
Løkken, Camilla
Rootwelt, Helge
Rønning, Per Ola
Wilson, Steven Ray
Elgstøen, Katja Benedikte Prestø
author_sort Skogvold, Hanne Bendiksen
collection PubMed
description [Image: see text] Dried blood spot (DBS) metabolite analysis is a central tool for the clinic, e.g., newborn screening. Instead of applying multiple analytical methods, a single liquid chromatography-mass spectrometry (LC–MS) method was developed for metabolites spanning from highly polar glucose to hydrophobic long-chain acylcarnitines. For liquid chromatography, a diphenyl column and a multi-linear solvent gradient operated at elevated flow rates allowed for an even-spread resolution of diverse metabolites. Injecting moderate volumes of DBS organic extracts directly, in contrast to evaporation and reconstitution, provided substantial increases in analyte recovery. Q Exactive MS settings were also tailored for sensitivity increases, and the method allowed for analyte retention time and peak area repeatabilities of 0.1–0.4 and 2–10%, respectively, for a wide polarity range of metabolites (log P −4.4 to 8.8). The method’s performance was suited for both untargeted analysis and targeted approaches evaluated in clinically relevant experiments.
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spelling pubmed-83974342021-08-31 Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes Skogvold, Hanne Bendiksen Sandås, Elise Mørk Østeby, Anja Løkken, Camilla Rootwelt, Helge Rønning, Per Ola Wilson, Steven Ray Elgstøen, Katja Benedikte Prestø J Proteome Res [Image: see text] Dried blood spot (DBS) metabolite analysis is a central tool for the clinic, e.g., newborn screening. Instead of applying multiple analytical methods, a single liquid chromatography-mass spectrometry (LC–MS) method was developed for metabolites spanning from highly polar glucose to hydrophobic long-chain acylcarnitines. For liquid chromatography, a diphenyl column and a multi-linear solvent gradient operated at elevated flow rates allowed for an even-spread resolution of diverse metabolites. Injecting moderate volumes of DBS organic extracts directly, in contrast to evaporation and reconstitution, provided substantial increases in analyte recovery. Q Exactive MS settings were also tailored for sensitivity increases, and the method allowed for analyte retention time and peak area repeatabilities of 0.1–0.4 and 2–10%, respectively, for a wide polarity range of metabolites (log P −4.4 to 8.8). The method’s performance was suited for both untargeted analysis and targeted approaches evaluated in clinically relevant experiments. American Chemical Society 2021-07-23 2021-08-06 /pmc/articles/PMC8397434/ /pubmed/34296888 http://dx.doi.org/10.1021/acs.jproteome.1c00326 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Skogvold, Hanne Bendiksen
Sandås, Elise Mørk
Østeby, Anja
Løkken, Camilla
Rootwelt, Helge
Rønning, Per Ola
Wilson, Steven Ray
Elgstøen, Katja Benedikte Prestø
Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes
title Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes
title_full Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes
title_fullStr Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes
title_full_unstemmed Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes
title_short Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes
title_sort bridging the polar and hydrophobic metabolome in single-run untargeted liquid chromatography-mass spectrometry dried blood spot metabolomics for clinical purposes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397434/
https://www.ncbi.nlm.nih.gov/pubmed/34296888
http://dx.doi.org/10.1021/acs.jproteome.1c00326
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