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3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis

Lumen formation of salivary glands has been investigated using in vivo or ex vivo rudiment culture models. In this study, we used a three-dimensional (3D) salivary gland organoid culture system and demonstrated that lumen formation could be recapitulated in mouse SMG organoids. In our organoid cultu...

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Autores principales: Kim, Donghyun, Yoon, Yeo-Jun, Choi, Dojin, Kim, Jisun, Lim, Jae-Yol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397473/
https://www.ncbi.nlm.nih.gov/pubmed/34458260
http://dx.doi.org/10.3389/fcell.2021.698292
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author Kim, Donghyun
Yoon, Yeo-Jun
Choi, Dojin
Kim, Jisun
Lim, Jae-Yol
author_facet Kim, Donghyun
Yoon, Yeo-Jun
Choi, Dojin
Kim, Jisun
Lim, Jae-Yol
author_sort Kim, Donghyun
collection PubMed
description Lumen formation of salivary glands has been investigated using in vivo or ex vivo rudiment culture models. In this study, we used a three-dimensional (3D) salivary gland organoid culture system and demonstrated that lumen formation could be recapitulated in mouse SMG organoids. In our organoid culture system, lumen formation was induced by vasoactive intestinal peptide and accelerated by treatment with RA. Furthermore, lumen formation was observed in branching duct-like structure when cultured in combination of fibroblast growth factors (FGF) in the presence of retinoic acid (RA). We suggest RA signaling-mediated regulation of VIPR1 and KRT7 as the underlying mechanism for lumen formation, rather than apoptosis in the organoid culture system. Collectively, our results support a fundamental role for RA in lumen formation and demonstrate the feasibility of 3D organoid culture as a tool for studying salivary gland morphogenesis.
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spelling pubmed-83974732021-08-28 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis Kim, Donghyun Yoon, Yeo-Jun Choi, Dojin Kim, Jisun Lim, Jae-Yol Front Cell Dev Biol Cell and Developmental Biology Lumen formation of salivary glands has been investigated using in vivo or ex vivo rudiment culture models. In this study, we used a three-dimensional (3D) salivary gland organoid culture system and demonstrated that lumen formation could be recapitulated in mouse SMG organoids. In our organoid culture system, lumen formation was induced by vasoactive intestinal peptide and accelerated by treatment with RA. Furthermore, lumen formation was observed in branching duct-like structure when cultured in combination of fibroblast growth factors (FGF) in the presence of retinoic acid (RA). We suggest RA signaling-mediated regulation of VIPR1 and KRT7 as the underlying mechanism for lumen formation, rather than apoptosis in the organoid culture system. Collectively, our results support a fundamental role for RA in lumen formation and demonstrate the feasibility of 3D organoid culture as a tool for studying salivary gland morphogenesis. Frontiers Media S.A. 2021-08-12 /pmc/articles/PMC8397473/ /pubmed/34458260 http://dx.doi.org/10.3389/fcell.2021.698292 Text en Copyright © 2021 Kim, Yoon, Choi, Kim and Lim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Kim, Donghyun
Yoon, Yeo-Jun
Choi, Dojin
Kim, Jisun
Lim, Jae-Yol
3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis
title 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis
title_full 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis
title_fullStr 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis
title_full_unstemmed 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis
title_short 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis
title_sort 3d organoid culture from adult salivary gland tissues as an ex vivo modeling of salivary gland morphogenesis
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397473/
https://www.ncbi.nlm.nih.gov/pubmed/34458260
http://dx.doi.org/10.3389/fcell.2021.698292
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