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Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate

This study aimed to investigate cerebral cortex apoptosis on the early aged hypertension and the effects of green tea flavonoid epigallocatechin-3-gallate (EGCG). Twenty-four rats were divided into three groups: a control Wistar-Kyoto group (WKY, n = 8), a spontaneously early aged hypertensive group...

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Autores principales: Hsieh, Min-Huang, Cui, Zhen-Yang, Yang, Ai-Lun, Nhu, Nguyen Thanh, Ting, Shih-Ying, Yu, Shao-Hong, Cheng, Yu-Jung, Lin, Yi-Yuan, Wu, Xu-Bo, Lee, Shin-Da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397540/
https://www.ncbi.nlm.nih.gov/pubmed/34456710
http://dx.doi.org/10.3389/fnagi.2021.705304
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author Hsieh, Min-Huang
Cui, Zhen-Yang
Yang, Ai-Lun
Nhu, Nguyen Thanh
Ting, Shih-Ying
Yu, Shao-Hong
Cheng, Yu-Jung
Lin, Yi-Yuan
Wu, Xu-Bo
Lee, Shin-Da
author_facet Hsieh, Min-Huang
Cui, Zhen-Yang
Yang, Ai-Lun
Nhu, Nguyen Thanh
Ting, Shih-Ying
Yu, Shao-Hong
Cheng, Yu-Jung
Lin, Yi-Yuan
Wu, Xu-Bo
Lee, Shin-Da
author_sort Hsieh, Min-Huang
collection PubMed
description This study aimed to investigate cerebral cortex apoptosis on the early aged hypertension and the effects of green tea flavonoid epigallocatechin-3-gallate (EGCG). Twenty-four rats were divided into three groups: a control Wistar-Kyoto group (WKY, n = 8), a spontaneously early aged hypertensive group (SHR, n = 8), and an early aged hypertension with EGCG treatment group (SHR-EGCG, n = 8; daily oral EGCG 200 mg/kg—94%, 12 weeks). At 48 weeks old, blood pressures (BPs) were evaluated and cerebral cortexes were isolated for TUNEL assay and Western blotting. Systolic, diastolic, and mean blood pressure levels in the SHR-EGCG were reduced compared to the SHR. The percentage of neural cell deaths, the levels of cytosolic Endonuclease G, cytosolic AIF (Caspase-independent apoptotic pathway), Fas, Fas Ligand, FADD, Caspase-8 (Fas-mediated apoptotic pathway), t-Bid, Bax/Bcl-2, Bak/Bcl-xL, cytosolic Cytochrome C, Apaf-1, Caspase-9 (Mitochondrial-mediated apoptotic pathway), and Caspase-3 (Fas-mediated and Mitochondria-mediated apoptotic pathways) were increased in the SHR relative to WKY and reduced in SHR-EGCG relative to SHR. In contrast, the levels of Bcl-2, Bcl-xL, p-Bad, 14-3-3, Bcl-2/Bax, Bcl-xL/Bak, and p-Bad/Bad (Bcl-2 family-related pro-survival pathway), as well as Sirt1, p-PI3K/PI3K and p-AKT/AKT (Sirt1/PI3K/AKT-related pro-survival pathway), were reduced in SHR relative WKY and enhanced in SHR-EGCG relative to SHR. In conclusion, green tea flavonoid epigallocatechin-3-gallate (EGCG) might prevent neural apoptotic pathways and activate neural survival pathways, providing therapeutic effects on early aged hypertension-induced neural apoptosis.
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spelling pubmed-83975402021-08-28 Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate Hsieh, Min-Huang Cui, Zhen-Yang Yang, Ai-Lun Nhu, Nguyen Thanh Ting, Shih-Ying Yu, Shao-Hong Cheng, Yu-Jung Lin, Yi-Yuan Wu, Xu-Bo Lee, Shin-Da Front Aging Neurosci Neuroscience This study aimed to investigate cerebral cortex apoptosis on the early aged hypertension and the effects of green tea flavonoid epigallocatechin-3-gallate (EGCG). Twenty-four rats were divided into three groups: a control Wistar-Kyoto group (WKY, n = 8), a spontaneously early aged hypertensive group (SHR, n = 8), and an early aged hypertension with EGCG treatment group (SHR-EGCG, n = 8; daily oral EGCG 200 mg/kg—94%, 12 weeks). At 48 weeks old, blood pressures (BPs) were evaluated and cerebral cortexes were isolated for TUNEL assay and Western blotting. Systolic, diastolic, and mean blood pressure levels in the SHR-EGCG were reduced compared to the SHR. The percentage of neural cell deaths, the levels of cytosolic Endonuclease G, cytosolic AIF (Caspase-independent apoptotic pathway), Fas, Fas Ligand, FADD, Caspase-8 (Fas-mediated apoptotic pathway), t-Bid, Bax/Bcl-2, Bak/Bcl-xL, cytosolic Cytochrome C, Apaf-1, Caspase-9 (Mitochondrial-mediated apoptotic pathway), and Caspase-3 (Fas-mediated and Mitochondria-mediated apoptotic pathways) were increased in the SHR relative to WKY and reduced in SHR-EGCG relative to SHR. In contrast, the levels of Bcl-2, Bcl-xL, p-Bad, 14-3-3, Bcl-2/Bax, Bcl-xL/Bak, and p-Bad/Bad (Bcl-2 family-related pro-survival pathway), as well as Sirt1, p-PI3K/PI3K and p-AKT/AKT (Sirt1/PI3K/AKT-related pro-survival pathway), were reduced in SHR relative WKY and enhanced in SHR-EGCG relative to SHR. In conclusion, green tea flavonoid epigallocatechin-3-gallate (EGCG) might prevent neural apoptotic pathways and activate neural survival pathways, providing therapeutic effects on early aged hypertension-induced neural apoptosis. Frontiers Media S.A. 2021-08-12 /pmc/articles/PMC8397540/ /pubmed/34456710 http://dx.doi.org/10.3389/fnagi.2021.705304 Text en Copyright © 2021 Hsieh, Cui, Yang, Nhu, Ting, Yu, Cheng, Lin, Wu and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Hsieh, Min-Huang
Cui, Zhen-Yang
Yang, Ai-Lun
Nhu, Nguyen Thanh
Ting, Shih-Ying
Yu, Shao-Hong
Cheng, Yu-Jung
Lin, Yi-Yuan
Wu, Xu-Bo
Lee, Shin-Da
Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate
title Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate
title_full Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate
title_fullStr Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate
title_full_unstemmed Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate
title_short Cerebral Cortex Apoptosis in Early Aged Hypertension: Effects of Epigallocatechin-3-Gallate
title_sort cerebral cortex apoptosis in early aged hypertension: effects of epigallocatechin-3-gallate
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397540/
https://www.ncbi.nlm.nih.gov/pubmed/34456710
http://dx.doi.org/10.3389/fnagi.2021.705304
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