Cargando…
Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCT...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397738/ https://www.ncbi.nlm.nih.gov/pubmed/34453033 http://dx.doi.org/10.1038/s41419-021-04091-y |
_version_ | 1783744680685469696 |
---|---|
author | Yang, Dae-Wook Mok, Jung-Wan Telerman, Stephanie B. Amson, Robert Telerman, Adam Choi, Kwang-Wook |
author_facet | Yang, Dae-Wook Mok, Jung-Wan Telerman, Stephanie B. Amson, Robert Telerman, Adam Choi, Kwang-Wook |
author_sort | Yang, Dae-Wook |
collection | PubMed |
description | Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCTP partner, but its role in vivo has been unknown. To determine the significance of this interaction, we examined their roles in developing Drosophila organs. Top2 RNAi in the wing disc leads to tissue reduction and caspase activation, indicating the essential role of Top2 for cell survival. Top2 RNAi in the eye disc also causes loss of eye and head tissues. Tctp RNAi enhances the phenotypes of Top2 RNAi. The depletion of Tctp reduces Top2 levels in the wing disc and vice versa. Wing size is reduced by Top2 overexpression, implying that proper regulation of Top2 level is important for normal organ development. The wing phenotype of Tctp RNAi is partially suppressed by Top2 overexpression. This study suggests that mutual regulation of Tctp and Top2 protein levels is critical for cell survival during organ development. |
format | Online Article Text |
id | pubmed-8397738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83977382021-09-15 Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila Yang, Dae-Wook Mok, Jung-Wan Telerman, Stephanie B. Amson, Robert Telerman, Adam Choi, Kwang-Wook Cell Death Dis Article Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCTP partner, but its role in vivo has been unknown. To determine the significance of this interaction, we examined their roles in developing Drosophila organs. Top2 RNAi in the wing disc leads to tissue reduction and caspase activation, indicating the essential role of Top2 for cell survival. Top2 RNAi in the eye disc also causes loss of eye and head tissues. Tctp RNAi enhances the phenotypes of Top2 RNAi. The depletion of Tctp reduces Top2 levels in the wing disc and vice versa. Wing size is reduced by Top2 overexpression, implying that proper regulation of Top2 level is important for normal organ development. The wing phenotype of Tctp RNAi is partially suppressed by Top2 overexpression. This study suggests that mutual regulation of Tctp and Top2 protein levels is critical for cell survival during organ development. Nature Publishing Group UK 2021-08-27 /pmc/articles/PMC8397738/ /pubmed/34453033 http://dx.doi.org/10.1038/s41419-021-04091-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Dae-Wook Mok, Jung-Wan Telerman, Stephanie B. Amson, Robert Telerman, Adam Choi, Kwang-Wook Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila |
title | Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila |
title_full | Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila |
title_fullStr | Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila |
title_full_unstemmed | Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila |
title_short | Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila |
title_sort | topoisomerase ii is regulated by translationally controlled tumor protein for cell survival during organ growth in drosophila |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397738/ https://www.ncbi.nlm.nih.gov/pubmed/34453033 http://dx.doi.org/10.1038/s41419-021-04091-y |
work_keys_str_mv | AT yangdaewook topoisomeraseiiisregulatedbytranslationallycontrolledtumorproteinforcellsurvivalduringorgangrowthindrosophila AT mokjungwan topoisomeraseiiisregulatedbytranslationallycontrolledtumorproteinforcellsurvivalduringorgangrowthindrosophila AT telermanstephanieb topoisomeraseiiisregulatedbytranslationallycontrolledtumorproteinforcellsurvivalduringorgangrowthindrosophila AT amsonrobert topoisomeraseiiisregulatedbytranslationallycontrolledtumorproteinforcellsurvivalduringorgangrowthindrosophila AT telermanadam topoisomeraseiiisregulatedbytranslationallycontrolledtumorproteinforcellsurvivalduringorgangrowthindrosophila AT choikwangwook topoisomeraseiiisregulatedbytranslationallycontrolledtumorproteinforcellsurvivalduringorgangrowthindrosophila |