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Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila

Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCT...

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Autores principales: Yang, Dae-Wook, Mok, Jung-Wan, Telerman, Stephanie B., Amson, Robert, Telerman, Adam, Choi, Kwang-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397738/
https://www.ncbi.nlm.nih.gov/pubmed/34453033
http://dx.doi.org/10.1038/s41419-021-04091-y
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author Yang, Dae-Wook
Mok, Jung-Wan
Telerman, Stephanie B.
Amson, Robert
Telerman, Adam
Choi, Kwang-Wook
author_facet Yang, Dae-Wook
Mok, Jung-Wan
Telerman, Stephanie B.
Amson, Robert
Telerman, Adam
Choi, Kwang-Wook
author_sort Yang, Dae-Wook
collection PubMed
description Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCTP partner, but its role in vivo has been unknown. To determine the significance of this interaction, we examined their roles in developing Drosophila organs. Top2 RNAi in the wing disc leads to tissue reduction and caspase activation, indicating the essential role of Top2 for cell survival. Top2 RNAi in the eye disc also causes loss of eye and head tissues. Tctp RNAi enhances the phenotypes of Top2 RNAi. The depletion of Tctp reduces Top2 levels in the wing disc and vice versa. Wing size is reduced by Top2 overexpression, implying that proper regulation of Top2 level is important for normal organ development. The wing phenotype of Tctp RNAi is partially suppressed by Top2 overexpression. This study suggests that mutual regulation of Tctp and Top2 protein levels is critical for cell survival during organ development.
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spelling pubmed-83977382021-09-15 Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila Yang, Dae-Wook Mok, Jung-Wan Telerman, Stephanie B. Amson, Robert Telerman, Adam Choi, Kwang-Wook Cell Death Dis Article Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCTP partner, but its role in vivo has been unknown. To determine the significance of this interaction, we examined their roles in developing Drosophila organs. Top2 RNAi in the wing disc leads to tissue reduction and caspase activation, indicating the essential role of Top2 for cell survival. Top2 RNAi in the eye disc also causes loss of eye and head tissues. Tctp RNAi enhances the phenotypes of Top2 RNAi. The depletion of Tctp reduces Top2 levels in the wing disc and vice versa. Wing size is reduced by Top2 overexpression, implying that proper regulation of Top2 level is important for normal organ development. The wing phenotype of Tctp RNAi is partially suppressed by Top2 overexpression. This study suggests that mutual regulation of Tctp and Top2 protein levels is critical for cell survival during organ development. Nature Publishing Group UK 2021-08-27 /pmc/articles/PMC8397738/ /pubmed/34453033 http://dx.doi.org/10.1038/s41419-021-04091-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yang, Dae-Wook
Mok, Jung-Wan
Telerman, Stephanie B.
Amson, Robert
Telerman, Adam
Choi, Kwang-Wook
Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
title Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
title_full Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
title_fullStr Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
title_full_unstemmed Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
title_short Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
title_sort topoisomerase ii is regulated by translationally controlled tumor protein for cell survival during organ growth in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397738/
https://www.ncbi.nlm.nih.gov/pubmed/34453033
http://dx.doi.org/10.1038/s41419-021-04091-y
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