Cargando…
Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography
As productive biofilms are increasingly gaining interest in research, the quantitative monitoring of biofilm formation on- or offline for the process remains a challenge. Optical coherence tomography (OCT) is a fast and often used method for scanning biofilms, but it has difficulty scanning through...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398007/ https://www.ncbi.nlm.nih.gov/pubmed/34442822 http://dx.doi.org/10.3390/microorganisms9081743 |
_version_ | 1783744735430574080 |
---|---|
author | Schaefer, Susanne Walther, Jakob Strieth, Dorina Ulber, Roland Bröckel, Ulrich |
author_facet | Schaefer, Susanne Walther, Jakob Strieth, Dorina Ulber, Roland Bröckel, Ulrich |
author_sort | Schaefer, Susanne |
collection | PubMed |
description | As productive biofilms are increasingly gaining interest in research, the quantitative monitoring of biofilm formation on- or offline for the process remains a challenge. Optical coherence tomography (OCT) is a fast and often used method for scanning biofilms, but it has difficulty scanning through more dense optical materials. X-ray microtomography (μCT) can measure biofilms in most geometries but is very time-consuming. By combining both methods for the first time, the weaknesses of both methods could be compensated. The phototrophic cyanobacterium Tolypothrix distorta was cultured in a moving bed photobioreactor inside a biocarrier with a semi-enclosed geometry. An automated workflow was developed to process µCT scans of the biocarriers. This allowed quantification of biomass volume and biofilm-coverage on the biocarrier, both globally and spatially resolved. At the beginning of the cultivation, a growth limitation was detected in the outer region of the carrier, presumably due to shear stress. In the later phase, light limitations could be found inside the biocarrier. µCT data and biofilm thicknesses measured by OCT displayed good correlation. The latter could therefore be used to rapidly measure the biofilm formation in a process. The methods presented here can help gain a deeper understanding of biofilms inside a process and detect any limitations. |
format | Online Article Text |
id | pubmed-8398007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83980072021-08-29 Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography Schaefer, Susanne Walther, Jakob Strieth, Dorina Ulber, Roland Bröckel, Ulrich Microorganisms Article As productive biofilms are increasingly gaining interest in research, the quantitative monitoring of biofilm formation on- or offline for the process remains a challenge. Optical coherence tomography (OCT) is a fast and often used method for scanning biofilms, but it has difficulty scanning through more dense optical materials. X-ray microtomography (μCT) can measure biofilms in most geometries but is very time-consuming. By combining both methods for the first time, the weaknesses of both methods could be compensated. The phototrophic cyanobacterium Tolypothrix distorta was cultured in a moving bed photobioreactor inside a biocarrier with a semi-enclosed geometry. An automated workflow was developed to process µCT scans of the biocarriers. This allowed quantification of biomass volume and biofilm-coverage on the biocarrier, both globally and spatially resolved. At the beginning of the cultivation, a growth limitation was detected in the outer region of the carrier, presumably due to shear stress. In the later phase, light limitations could be found inside the biocarrier. µCT data and biofilm thicknesses measured by OCT displayed good correlation. The latter could therefore be used to rapidly measure the biofilm formation in a process. The methods presented here can help gain a deeper understanding of biofilms inside a process and detect any limitations. MDPI 2021-08-16 /pmc/articles/PMC8398007/ /pubmed/34442822 http://dx.doi.org/10.3390/microorganisms9081743 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schaefer, Susanne Walther, Jakob Strieth, Dorina Ulber, Roland Bröckel, Ulrich Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography |
title | Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography |
title_full | Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography |
title_fullStr | Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography |
title_full_unstemmed | Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography |
title_short | Insights into the Development of Phototrophic Biofilms in a Bioreactor by a Combination of X-ray Microtomography and Optical Coherence Tomography |
title_sort | insights into the development of phototrophic biofilms in a bioreactor by a combination of x-ray microtomography and optical coherence tomography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398007/ https://www.ncbi.nlm.nih.gov/pubmed/34442822 http://dx.doi.org/10.3390/microorganisms9081743 |
work_keys_str_mv | AT schaefersusanne insightsintothedevelopmentofphototrophicbiofilmsinabioreactorbyacombinationofxraymicrotomographyandopticalcoherencetomography AT waltherjakob insightsintothedevelopmentofphototrophicbiofilmsinabioreactorbyacombinationofxraymicrotomographyandopticalcoherencetomography AT striethdorina insightsintothedevelopmentofphototrophicbiofilmsinabioreactorbyacombinationofxraymicrotomographyandopticalcoherencetomography AT ulberroland insightsintothedevelopmentofphototrophicbiofilmsinabioreactorbyacombinationofxraymicrotomographyandopticalcoherencetomography AT brockelulrich insightsintothedevelopmentofphototrophicbiofilmsinabioreactorbyacombinationofxraymicrotomographyandopticalcoherencetomography |