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The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge

The dinitrotoluene isomers 2,4 and 2,6-dinitrotoluene (DNT) represent highly toxic, mutagenic, and carcinogenic compounds used in explosive manufacturing and in commercial production of polyurethane foam. Bioremediation, the use of microbes to degrade residual DNT in industry wastewaters, represents...

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Autores principales: Aburto-Medina, Arturo, Shahsavari, Esmaeil, Taha, Mohamed, Bates, Andrew, Van Ieperen, Leon, Ball, Andrew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398102/
https://www.ncbi.nlm.nih.gov/pubmed/34443401
http://dx.doi.org/10.3390/molecules26164814
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author Aburto-Medina, Arturo
Shahsavari, Esmaeil
Taha, Mohamed
Bates, Andrew
Van Ieperen, Leon
Ball, Andrew S.
author_facet Aburto-Medina, Arturo
Shahsavari, Esmaeil
Taha, Mohamed
Bates, Andrew
Van Ieperen, Leon
Ball, Andrew S.
author_sort Aburto-Medina, Arturo
collection PubMed
description The dinitrotoluene isomers 2,4 and 2,6-dinitrotoluene (DNT) represent highly toxic, mutagenic, and carcinogenic compounds used in explosive manufacturing and in commercial production of polyurethane foam. Bioremediation, the use of microbes to degrade residual DNT in industry wastewaters, represents a promising, low cost and environmentally friendly alternative technology to landfilling. In the present study, the effect of different bioremediation strategies on the degradation of DNT in a microcosm-based study was evaluated. Biostimulation of the indigenous microbial community with sulphur phosphate (2.3 g/kg sludge) enhanced DNT transformation (82% transformation, from 300 g/L at Day 0 to 55 g/L in week 6) compared to natural attenuation over the same period at 25 °C. The indigenous microbial activity was found to be capable of transforming the contaminant, with around 70% transformation of DNT occurring over the microcosm study. 16S rDNA sequence analysis revealed that while the original bacterial community was dominated by Gammaproteobacteria (30%), the addition of sulphur phosphate significantly increased the abundance of Betaproteobacteria by the end of the biostimulation treatment, with the bacterial community dominated by Burkholderia (46%) followed by Rhodanobacter, Acidovorax and Pseudomonas. In summary, the results suggest biostimulation as a treatment choice for the remediation of dinitrotoluenes and explosives waste.
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spelling pubmed-83981022021-08-29 The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge Aburto-Medina, Arturo Shahsavari, Esmaeil Taha, Mohamed Bates, Andrew Van Ieperen, Leon Ball, Andrew S. Molecules Article The dinitrotoluene isomers 2,4 and 2,6-dinitrotoluene (DNT) represent highly toxic, mutagenic, and carcinogenic compounds used in explosive manufacturing and in commercial production of polyurethane foam. Bioremediation, the use of microbes to degrade residual DNT in industry wastewaters, represents a promising, low cost and environmentally friendly alternative technology to landfilling. In the present study, the effect of different bioremediation strategies on the degradation of DNT in a microcosm-based study was evaluated. Biostimulation of the indigenous microbial community with sulphur phosphate (2.3 g/kg sludge) enhanced DNT transformation (82% transformation, from 300 g/L at Day 0 to 55 g/L in week 6) compared to natural attenuation over the same period at 25 °C. The indigenous microbial activity was found to be capable of transforming the contaminant, with around 70% transformation of DNT occurring over the microcosm study. 16S rDNA sequence analysis revealed that while the original bacterial community was dominated by Gammaproteobacteria (30%), the addition of sulphur phosphate significantly increased the abundance of Betaproteobacteria by the end of the biostimulation treatment, with the bacterial community dominated by Burkholderia (46%) followed by Rhodanobacter, Acidovorax and Pseudomonas. In summary, the results suggest biostimulation as a treatment choice for the remediation of dinitrotoluenes and explosives waste. MDPI 2021-08-09 /pmc/articles/PMC8398102/ /pubmed/34443401 http://dx.doi.org/10.3390/molecules26164814 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aburto-Medina, Arturo
Shahsavari, Esmaeil
Taha, Mohamed
Bates, Andrew
Van Ieperen, Leon
Ball, Andrew S.
The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
title The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
title_full The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
title_fullStr The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
title_full_unstemmed The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
title_short The Impacts of Different Biological Treatments on the Transformation of Explosives Waste Contaminated Sludge
title_sort impacts of different biological treatments on the transformation of explosives waste contaminated sludge
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398102/
https://www.ncbi.nlm.nih.gov/pubmed/34443401
http://dx.doi.org/10.3390/molecules26164814
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