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PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

Ionic substitutions within the hydroxyapatite lattice are a widely used approach to mimic the chemical composition of the bone mineral. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate (CaP) scaffolds, with various levels of strontium and magnesium substitution, were prep...

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Autores principales: Bauer, Leonard, Antunović, Maja, Gallego-Ferrer, Gloria, Ivanković, Marica, Ivanković, Hrvoje
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398152/
https://www.ncbi.nlm.nih.gov/pubmed/34442926
http://dx.doi.org/10.3390/ma14164403
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author Bauer, Leonard
Antunović, Maja
Gallego-Ferrer, Gloria
Ivanković, Marica
Ivanković, Hrvoje
author_facet Bauer, Leonard
Antunović, Maja
Gallego-Ferrer, Gloria
Ivanković, Marica
Ivanković, Hrvoje
author_sort Bauer, Leonard
collection PubMed
description Ionic substitutions within the hydroxyapatite lattice are a widely used approach to mimic the chemical composition of the bone mineral. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate (CaP) scaffolds, with various levels of strontium and magnesium substitution, were prepared using the hydrothermal method at 200 °C. Calcium carbonate skeletons of cuttlefish bone, ammonium dihydrogenphosphate (NH(4)H(2)PO(4)), strontium nitrate (Sr(NO(3))(2)), and magnesium perchlorate (Mg(ClO(4))(2)) were used as reagents. Materials were characterized by means of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Whole powder pattern decomposition refinements of XRD data indicated that increased magnesium content in the Mg- and Sr-co-substituted scaffolds was related to an increased proportion of the whitlockite (WH) phase in the biphasic hydroxyapatite (HAp)/WH scaffolds. In addition, refinements indicate that Sr(2+) ions have replaced Ca(2+) sites in the WH phase. Furthermore, PCL-coated Mg-substituted and Sr- and Mg-co-substituted scaffolds, with the HAp:WH wt. ratio of 90:10 were prepared by vacuum impregnation. Results of compression tests showed a positive impact of the WH phase and PCL coating on the mechanical properties of scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on composite scaffolds in an osteogenic medium for 21 days. Immunohistochemical staining showed that Mg-Sr-CaP/PCL scaffold exhibited higher expression of collagen type I than the Mg-CaP/PCL scaffold, indicating the positive effect of Sr(2+) ions on the differentiation of hMSCs, in concordance with histology results. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis confirmed an early stage of osteogenic differentiation.
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spelling pubmed-83981522021-08-29 PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties Bauer, Leonard Antunović, Maja Gallego-Ferrer, Gloria Ivanković, Marica Ivanković, Hrvoje Materials (Basel) Article Ionic substitutions within the hydroxyapatite lattice are a widely used approach to mimic the chemical composition of the bone mineral. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate (CaP) scaffolds, with various levels of strontium and magnesium substitution, were prepared using the hydrothermal method at 200 °C. Calcium carbonate skeletons of cuttlefish bone, ammonium dihydrogenphosphate (NH(4)H(2)PO(4)), strontium nitrate (Sr(NO(3))(2)), and magnesium perchlorate (Mg(ClO(4))(2)) were used as reagents. Materials were characterized by means of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Whole powder pattern decomposition refinements of XRD data indicated that increased magnesium content in the Mg- and Sr-co-substituted scaffolds was related to an increased proportion of the whitlockite (WH) phase in the biphasic hydroxyapatite (HAp)/WH scaffolds. In addition, refinements indicate that Sr(2+) ions have replaced Ca(2+) sites in the WH phase. Furthermore, PCL-coated Mg-substituted and Sr- and Mg-co-substituted scaffolds, with the HAp:WH wt. ratio of 90:10 were prepared by vacuum impregnation. Results of compression tests showed a positive impact of the WH phase and PCL coating on the mechanical properties of scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on composite scaffolds in an osteogenic medium for 21 days. Immunohistochemical staining showed that Mg-Sr-CaP/PCL scaffold exhibited higher expression of collagen type I than the Mg-CaP/PCL scaffold, indicating the positive effect of Sr(2+) ions on the differentiation of hMSCs, in concordance with histology results. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis confirmed an early stage of osteogenic differentiation. MDPI 2021-08-06 /pmc/articles/PMC8398152/ /pubmed/34442926 http://dx.doi.org/10.3390/ma14164403 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bauer, Leonard
Antunović, Maja
Gallego-Ferrer, Gloria
Ivanković, Marica
Ivanković, Hrvoje
PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties
title PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties
title_full PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties
title_fullStr PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties
title_full_unstemmed PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties
title_short PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties
title_sort pcl-coated multi-substituted calcium phosphate bone scaffolds with enhanced properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398152/
https://www.ncbi.nlm.nih.gov/pubmed/34442926
http://dx.doi.org/10.3390/ma14164403
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