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Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

The objective of this study was to investigate the potential of titanium nanotubes to promote the proliferation of human osteoblasts and to reduce monomicrobial biofilm adhesion. A secondary objective was to determine the effect of silicon carbide (SiC) on these nanostructured surfaces. Anodized tit...

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Autores principales: Camargo, Samira Esteves Afonso, Xia, Xinyi, Fares, Chaker, Ren, Fan, Hsu, Shu-Min, Budei, Dragos, Aravindraja, Chairmandurai, Kesavalu, Lakshmyya, Esquivel-Upshaw, Josephine F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398300/
https://www.ncbi.nlm.nih.gov/pubmed/34442878
http://dx.doi.org/10.3390/ma14164357
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author Camargo, Samira Esteves Afonso
Xia, Xinyi
Fares, Chaker
Ren, Fan
Hsu, Shu-Min
Budei, Dragos
Aravindraja, Chairmandurai
Kesavalu, Lakshmyya
Esquivel-Upshaw, Josephine F.
author_facet Camargo, Samira Esteves Afonso
Xia, Xinyi
Fares, Chaker
Ren, Fan
Hsu, Shu-Min
Budei, Dragos
Aravindraja, Chairmandurai
Kesavalu, Lakshmyya
Esquivel-Upshaw, Josephine F.
author_sort Camargo, Samira Esteves Afonso
collection PubMed
description The objective of this study was to investigate the potential of titanium nanotubes to promote the proliferation of human osteoblasts and to reduce monomicrobial biofilm adhesion. A secondary objective was to determine the effect of silicon carbide (SiC) on these nanostructured surfaces. Anodized titanium sheets with 100–150 nm nanotubes were either coated or not coated with SiC. After 24 h of osteoblast cultivation on the samples, cells were observed on all titanium sheets by SEM. In addition, the cytotoxicity was evaluated by CellTiter-BlueCell assay after 1, 3, and 7 days. The samples were also cultivated in culture medium with microorganisms incubated anaerobically with respective predominant periodontal bacteria viz. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia as monoinfection at 37 °C for 30 days. The biofilm adhesion and coverage were evaluated through surface observation using Scanning Electron Microscopy (SEM). The results demonstrate that Ti nanostructured surfaces induced more cell proliferation after seven days. All groups presented no cytotoxic effects on human osteoblasts. In addition, SEM images illustrate that Ti nanostructured surfaces exhibited lower biofilm coverage compared to the reference samples. These results indicate that Ti nanotubes promoted osteoblasts proliferation and induced cell proliferation on the surface, compared with the controls. Ti nanotubes also reduced biofilm adhesion on titanium implant surfaces.
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spelling pubmed-83983002021-08-29 Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium Camargo, Samira Esteves Afonso Xia, Xinyi Fares, Chaker Ren, Fan Hsu, Shu-Min Budei, Dragos Aravindraja, Chairmandurai Kesavalu, Lakshmyya Esquivel-Upshaw, Josephine F. Materials (Basel) Article The objective of this study was to investigate the potential of titanium nanotubes to promote the proliferation of human osteoblasts and to reduce monomicrobial biofilm adhesion. A secondary objective was to determine the effect of silicon carbide (SiC) on these nanostructured surfaces. Anodized titanium sheets with 100–150 nm nanotubes were either coated or not coated with SiC. After 24 h of osteoblast cultivation on the samples, cells were observed on all titanium sheets by SEM. In addition, the cytotoxicity was evaluated by CellTiter-BlueCell assay after 1, 3, and 7 days. The samples were also cultivated in culture medium with microorganisms incubated anaerobically with respective predominant periodontal bacteria viz. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia as monoinfection at 37 °C for 30 days. The biofilm adhesion and coverage were evaluated through surface observation using Scanning Electron Microscopy (SEM). The results demonstrate that Ti nanostructured surfaces induced more cell proliferation after seven days. All groups presented no cytotoxic effects on human osteoblasts. In addition, SEM images illustrate that Ti nanostructured surfaces exhibited lower biofilm coverage compared to the reference samples. These results indicate that Ti nanotubes promoted osteoblasts proliferation and induced cell proliferation on the surface, compared with the controls. Ti nanotubes also reduced biofilm adhesion on titanium implant surfaces. MDPI 2021-08-04 /pmc/articles/PMC8398300/ /pubmed/34442878 http://dx.doi.org/10.3390/ma14164357 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Camargo, Samira Esteves Afonso
Xia, Xinyi
Fares, Chaker
Ren, Fan
Hsu, Shu-Min
Budei, Dragos
Aravindraja, Chairmandurai
Kesavalu, Lakshmyya
Esquivel-Upshaw, Josephine F.
Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium
title Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium
title_full Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium
title_fullStr Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium
title_full_unstemmed Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium
title_short Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium
title_sort nanostructured surfaces to promote osteoblast proliferation and minimize bacterial adhesion on titanium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398300/
https://www.ncbi.nlm.nih.gov/pubmed/34442878
http://dx.doi.org/10.3390/ma14164357
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